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Q89X45

- DAPF_BRADU

UniProt

Q89X45 - DAPF_BRADU

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Protein

Diaminopimelate epimerase

Gene
dapF, bll0477
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity.UniRule annotation

Catalytic activityi

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei17 – 171Substrate By similarity
Binding sitei50 – 501Substrate By similarity
Binding sitei70 – 701Substrate By similarity
Active sitei79 – 791Proton donor/acceptor By similarity
Binding sitei167 – 1671Substrate By similarity
Sitei169 – 1691Important for catalytic activity By similarity
Binding sitei200 – 2001Substrate By similarity
Sitei218 – 2181Important for catalytic activity By similarity
Active sitei227 – 2271Proton donor/acceptor By similarity

GO - Molecular functioni

  1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

Amino-acid biosynthesis, Lysine biosynthesis

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-480-MONOMER.
UniPathwayiUPA00034; UER00025.

Names & Taxonomyi

Protein namesi
Recommended name:
Diaminopimelate epimerase (EC:5.1.1.7)
Short name:
DAP epimerase
Gene namesi
Name:dapF
Ordered Locus Names:bll0477
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 291291Diaminopimelate epimeraseUniRule annotationPRO_0000149822Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi79 ↔ 227UniRule annotation

Keywords - PTMi

Disulfide bond

Interactioni

Protein-protein interaction databases

STRINGi224911.bll0477.

Structurei

3D structure databases

ProteinModelPortaliQ89X45.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni14 – 152Substrate binding By similarity
Regioni79 – 813Substrate binding By similarity
Regioni218 – 2192Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0253.
HOGENOMiHOG000220466.
KOiK01778.
OrthoDBiEOG6ND0M5.
PhylomeDBiQ89X45.

Family and domain databases

HAMAPiMF_00197. DAP_epimerase.
InterProiIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERiPTHR31689. PTHR31689. 1 hit.
PfamiPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsiTIGR00652. DapF. 1 hit.
PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89X45-1 [UniParc]FASTAAdd to Basket

« Hide

MSALANHAFA KMNGIGNEIV VVDMRDSTAK VTPDDARAVA SAQGGVPYDQ    50
LMVLQKPRLD GTEAFISIYN NDGSEAGACG NGMRCVVRRI FEKTGQATAT 100
FETAAGLLNA WQGPAPDLYT VDMGVPKFGW QDIPLAEEFR DTRYIELQIG 150
PIDNPILHSP SVVSMGNPHA IFWVDDVNAY DLARFGPLLE NHPIFPERAN 200
ITLAHIVDRD HITIRTWERG AGLTRACGSA ACATAVAAAR LKRAERNVEI 250
TLPGGKLGIE WRERDDHVLM TGTATFEYEG NFDPALFAPA G 291
Length:291
Mass (Da):31,595
Last modified:June 20, 2003 - v1
Checksum:i58112A152FA5D6E3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC45742.1.
RefSeqiNP_767117.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC45742; BAC45742; BAC45742.
GeneIDi1053475.
KEGGibja:bll0477.
PATRICi21184419. VBIBraJap65052_0487.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC45742.1 .
RefSeqi NP_767117.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89X45.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.bll0477.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC45742 ; BAC45742 ; BAC45742 .
GeneIDi 1053475.
KEGGi bja:bll0477.
PATRICi 21184419. VBIBraJap65052_0487.

Phylogenomic databases

eggNOGi COG0253.
HOGENOMi HOG000220466.
KOi K01778.
OrthoDBi EOG6ND0M5.
PhylomeDBi Q89X45.

Enzyme and pathway databases

UniPathwayi UPA00034 ; UER00025 .
BioCyci BJAP224911:GJEJ-480-MONOMER.

Family and domain databases

HAMAPi MF_00197. DAP_epimerase.
InterProi IPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view ]
PANTHERi PTHR31689. PTHR31689. 1 hit.
Pfami PF01678. DAP_epimerase. 2 hits.
[Graphical view ]
TIGRFAMsi TIGR00652. DapF. 1 hit.
PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiDAPF_BRADU
AccessioniPrimary (citable) accession number: Q89X45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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