Reviewed, UniProtKB/Swiss-Prot Q89X36 (TRMD_BRAJA)

Names and origin

Protein names

Recommended name:
    tRNA (guanine-N(1)-)-methyltransferase
    EC=2.1.1.31
Alternative name(s):
    M1G-methyltransferase
    tRNA [GM37] methyltransferase

Gene names

Name:	trmD
Ordered Locus Names:	blr0486

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically methylates guanosine-37 in various tRNAs By similarity.
Catalytic activity	S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(1)-methylguanine. HAMAP MF_00605
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the RNA methyltransferase trmD family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro tRNA (guanine-N1-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	tRNA (guanine-N(1)-)-methyltransferase HAMAP MF_00605		PRO_0000060341
Regions
	Region	141 – 146	6	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	121	1	S-adenosyl-L-methionine; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89X36-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 399DE5FC0FC9599D	FASTA	255	27,151
	10 20 30 40 50 60 MTTSSPWRAT VLTLFPEMFP GPLGVSLAGR ALASGLWQLE ARDIRASATD RHRSVDDTPA 70 80 90 100 110 120 GGGPGMVLRA DVLAAAIDAA EIGQGSSKER PRLLMSPRGR PLTQARVVEL ARGPGPLIVC 130 140 150 160 170 180 GRFEGIDQRV IDGRGLEEVS IGDYVLSGGE IAALALIDAC VRLLPGVMGK EASGTEESFS 190 200 210 220 230 240 DGLLEYPQYT RPQLFEGVPI PEILTSGDHA KVAAWRRAEA EALTAARRPD LWAQIPVKAP 250 NRAGRQKTPK NKTDG

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC45751.1.
RefSeq	NP_767126.1.
3D structure databases
HSSP	HSSP built from PDB template 1UAJ based on UniProtKB P43912.
ModBase	Search...
Genome annotation databases
GeneID	1049220.
GenomeReviews	Gene locus blr0486 in contig BA000040_GR.
KEGG	bja:blr0486.
NMPDR	fig|224911.1.peg.486.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89X36.
OMA	Q89X36. HRSVDDT.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR0486-MON.
BRENDA	2.1.1.31. 280.
Family and domain databases
HAMAP	MF_00605. [Tree]
InterPro	IPR016009. tRNA_m1G_MeTrfase. IPR002649. tRNA_m1G_MeTrfase_bac. [Graphical view]
Pfam	PF01746. tRNA_m1G_MT. 1 hit. [Graphical view]
PIRSF	PIRSF000386. tRNA_mtase. 1 hit.
ProDom	PD004978. tRNA_m1G_mtfrase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00088. trmD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMD_BRAJA

Accession

Primary (citable) accession number: Q89X36

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)