Reviewed, UniProtKB/Swiss-Prot Q89WY0 (IPYR_BRAJA)

Names and origin

Protein names

Recommended name:
    Inorganic pyrophosphatase
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate phospho-hydrolase
      Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	blr0548

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137481
Sites
	Metal binding	66	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 1 By similarity
	Metal binding	71	1	Magnesium 2 By similarity
	Metal binding	103	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89WY0-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: EFE8FBDF15D51EB1	FASTA	178	20,052
	10 20 30 40 50 60 MRIDAISIGK NVPQDVNVII EVPVGGEPIK YEMDKEAGTL VVDRFLYTPM RYPGNYGFIP 70 80 90 100 110 120 HTLSDDGDPC DVLIINTRAI IPGAVMSVRP VGVLFMEDEA GGDEKILAVP SSKLTQRYDK 130 140 150 160 170 VKSYSDLPDI TLQQIQHFFE HYKDLEKGKW VKILRWGGPE DAHKLILEGI EREKKKKG

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC45813.1.
RefSeq	NP_767188.1.
3D structure databases
HSSP	HSSP built from PDB template 1FAJ based on UniProtKB P17288.
ModBase	Search...
Genome annotation databases
GeneID	1049685.
GenomeReviews	Gene locus blr0548 in contig BA000040_GR.
KEGG	bja:blr0548.
NMPDR	fig|224911.1.peg.548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89WY0.
OMA	EHFFEHY.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR0548-MON.
BRENDA	3.6.1.1. 280.
Family and domain databases
HAMAP	MF_00209. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
ProDom	PD002014. Inorg_pphsph. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00387. PPASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_BRAJA

Accession

Primary (citable) accession number: Q89WY0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)