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Q89WU7 (PUR9_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional purine biosynthesis protein PurH

Including the following 2 domains:

  1. Phosphoribosylaminoimidazolecarboxamide formyltransferase
    EC=2.1.2.3
    Alternative name(s):
    AICAR transformylase
  2. IMP cyclohydrolase
    EC=3.5.4.10
    Alternative name(s):
    ATIC
    IMP synthase
    Inosinicase
Gene names
Name:purH
Ordered Locus Names:blr0581
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length530 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

IMP + H2O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_00139

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide (10-formyl THF route): step 1/1. HAMAP-Rule MF_00139

Purine metabolism; IMP biosynthesis via de novo pathway; IMP from 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide: step 1/1.

Domain

The IMP cyclohydrolase activity resides in the N-terminal region By similarity. HAMAP-Rule MF_00139

Sequence similarities

Belongs to the PurH family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 530530Bifunctional purine biosynthesis protein PurH HAMAP-Rule MF_00139
PRO_0000192073

Sequences

Sequence LengthMass (Da)Tools
Q89WU7 [UniParc].

Last modified June 20, 2003. Version 1.
Checksum: A7DD03550120605C

FASTA53056,465
        10         20         30         40         50         60 
MTDHPRRVTR ALLSVSDKTG LIEFAKALAA HDVELVSTGG TAKAIAAAGL KVKDVSELTG 

        70         80         90        100        110        120 
FPEMMDGRVK TLHPKVHGGL LAIRDNKDHA DAMKAHGIAP IDLLVVNLYP FEATVDKGAG 

       130        140        150        160        170        180 
FEDCIENIDI GGPAMIRAAA KNHDDVAVVV EAEDYKAVLD ELAANKGATT LKLRRRLAAK 

       190        200        210        220        230        240 
AYARTAAYDA AISNWFNRQL EIDAPDFRAF GGKLIQSLRY GENPHQTAAF YATPDKRPGV 

       250        260        270        280        290        300 
STARQLQGKE LSYNNINDTD AAYECIGEFD AKRTAACVIV KHANPCGVAE GSDLVSAYRK 

       310        320        330        340        350        360 
ALACDSTSAF GGIIAMNRAL DADTAREITK IFTEVIIAPD ASEEAIAIIG ARKNLRLLLA 

       370        380        390        400        410        420 
GSLPDPRAPG LTAKTVAGGL LVQSRDNAVV DDMTFKVVTK RAPTDAEMRD LKFAFRVAKH 

       430        440        450        460        470        480 
VKSNTIIYAK DLATVGIGAG QMSRVDSARI AARKAQDAAV ELKLAEPLTK GSVVASDAFF 

       490        500        510        520        530 
PFADGMLACI EAGATAVVQP GGSMRDDEVI KAADEHGIAM VFTGTRHFRH 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC45846.1.
RefSeqNP_767221.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89WU7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr0581.

Proteomic databases

PRIDEQ89WU7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC45846; BAC45846; BAC45846.
GeneID1049554.
KEGGbja:blr0581.
PATRIC21184645. VBIBraJap65052_0598.

Phylogenomic databases

eggNOGCOG0138.
HOGENOMHOG000230372.
KOK00602.
OrthoDBEOG6QCDFF.
PhylomeDBQ89WU7.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-586-MONOMER.
UniPathwayUPA00074; UER00133.
UPA00074; UER00135.

Family and domain databases

Gene3D3.40.140.20. 2 hits.
3.40.50.1380. 1 hit.
HAMAPMF_00139. PurH.
InterProIPR024051. AICAR_Tfase_dom.
IPR002695. AICARFT_IMPCHas.
IPR016193. Cytidine_deaminase-like.
IPR011607. MGS-like_dom.
[Graphical view]
PANTHERPTHR11692. PTHR11692. 1 hit.
PfamPF01808. AICARFT_IMPCHas. 1 hit.
PF02142. MGS. 1 hit.
[Graphical view]
PIRSFPIRSF000414. AICARFT_IMPCHas. 1 hit.
SMARTSM00798. AICARFT_IMPCHas. 1 hit.
SM00851. MGS. 1 hit.
[Graphical view]
SUPFAMSSF52335. SSF52335. 1 hit.
SSF53927. SSF53927. 1 hit.
TIGRFAMsTIGR00355. purH. 1 hit.
ProtoNetSearch...

Entry information

Entry namePUR9_BRADU
AccessionPrimary (citable) accession number: Q89WU7
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways