Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q89WQ1 (SYL_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:blr0627
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length874 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 874874Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000151984

Regions

Motif43 – 5311"HIGH" region HAMAP-Rule MF_00049
Motif630 – 6345"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6331ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89WQ1 [UniParc].

Last modified June 20, 2003. Version 1.
Checksum: 570F8A8F96A3663B

FASTA87497,908
        10         20         30         40         50         60 
MTSERYNARD SEPRWQAAWD EKAIFVSKND DSRPKYYVLE MFPYPSGRIH IGHVRNYTLG 

        70         80         90        100        110        120 
DVLARFMRAK GFNVLHPMGW DAFGLPAENA AIERKVAPKA WTYDNIAAMK KQLRSIGLSL 

       130        140        150        160        170        180 
DWSREIATCD PSYYKHQQKM FLDFLRAGLA EREKRKVNWD PVDMTVLANE QVIDGKGWRS 

       190        200        210        220        230        240 
GAIVEQREMN QWVFKITKYS QELLSALDTL DRWPDKVRLM QRNWIGRSEG LLVRFALDQA 

       250        260        270        280        290        300 
TTPAGESELK IFTTRPDTLF GAKFMAISAD HPLATAAAAK DPKLAEFIAE IKKIGTAQEI 

       310        320        330        340        350        360 
IDTAEKQGFD TGIRAIHPFD PSWKLPVYVA NFVLMEYGTG AIFGCPAHDQ RDLDFVNKYN 

       370        380        390        400        410        420 
LGNTPVVCPE GQDPKSFVIT DTAYDGDGRM INSRFLDGMT IEQAKDEVAK RLESELRGNA 

       430        440        450        460        470        480 
PVGERQVNFR LRDWGISRQR YWGCPIPVIH CPTCDVVPVP DADLPVKLPD DATFDKPGNA 

       490        500        510        520        530        540 
LDHHPTWKHV TCPQCGGKAQ RETDTMDTFV DSSWYFARFT DPWNENAPTT PAVANRMLPI 

       550        560        570        580        590        600 
DQYIGGVEHA ILHLLYSRFF TRAMKATGHV ALDEPFAGMF TQGMVVHETY QKADGSYVQP 

       610        620        630        640        650        660 
AEVKIELGGN GRRATLLTTG EDIQIGAIEK MSKSKKNTVD PDDIIETYGA DVARWFMLSD 

       670        680        690        700        710        720 
SPPDRDVIWS DERVQGASRF VQRLWRLVND SVELGKAAPA ARPASFGADA TALRKAAHGA 

       730        740        750        760        770        780 
LDKVTTEIER LHFNVCLAHI REFTNAFSEV LQRPGQPAAD LAWAIREASQ ILVQLFSPMM 

       790        800        810        820        830        840 
PHLAEECWQV LGQKGLVSEA NWPQIERDLL VEDSVTLVVQ VNGKKRGEVT VATAAQNPEI 

       850        860        870 
EAAVLALDAV KLALDGKPVR KVIIVPKRIV NVVG 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC45892.1.
RefSeqNP_767267.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89WQ1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr0627.

Proteomic databases

PRIDEQ89WQ1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC45892; BAC45892; BAC45892.
GeneID1049413.
KEGGbja:blr0627.
PATRIC21184742. VBIBraJap65052_0645.

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OrthoDBEOG63Z74X.
PhylomeDBQ89WQ1.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-633-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BRADU
AccessionPrimary (citable) accession number: Q89WQ1
Entry history
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries