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Q89WQ1

- SYL_BRADU

UniProt

Q89WQ1 - SYL_BRADU

Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (20 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei633 – 6331ATPUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-633-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
    Alternative name(s):
    Leucyl-tRNA synthetaseUniRule annotation
    Short name:
    LeuRSUniRule annotation
    Gene namesi
    Name:leuSUniRule annotation
    Ordered Locus Names:blr0627
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 874874Leucine--tRNA ligasePRO_0000151984Add
    BLAST

    Proteomic databases

    PRIDEiQ89WQ1.

    Interactioni

    Protein-protein interaction databases

    STRINGi224911.blr0627.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89WQ1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi43 – 5311"HIGH" regionAdd
    BLAST
    Motifi630 – 6345"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0495.
    HOGENOMiHOG000200747.
    KOiK01869.
    OrthoDBiEOG63Z74X.
    PhylomeDBiQ89WQ1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_00049_B. Leu_tRNA_synth_B.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view]
    PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR00985. TRNASYNTHLEU.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q89WQ1-1 [UniParc]FASTAAdd to Basket

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    MTSERYNARD SEPRWQAAWD EKAIFVSKND DSRPKYYVLE MFPYPSGRIH    50
    IGHVRNYTLG DVLARFMRAK GFNVLHPMGW DAFGLPAENA AIERKVAPKA 100
    WTYDNIAAMK KQLRSIGLSL DWSREIATCD PSYYKHQQKM FLDFLRAGLA 150
    EREKRKVNWD PVDMTVLANE QVIDGKGWRS GAIVEQREMN QWVFKITKYS 200
    QELLSALDTL DRWPDKVRLM QRNWIGRSEG LLVRFALDQA TTPAGESELK 250
    IFTTRPDTLF GAKFMAISAD HPLATAAAAK DPKLAEFIAE IKKIGTAQEI 300
    IDTAEKQGFD TGIRAIHPFD PSWKLPVYVA NFVLMEYGTG AIFGCPAHDQ 350
    RDLDFVNKYN LGNTPVVCPE GQDPKSFVIT DTAYDGDGRM INSRFLDGMT 400
    IEQAKDEVAK RLESELRGNA PVGERQVNFR LRDWGISRQR YWGCPIPVIH 450
    CPTCDVVPVP DADLPVKLPD DATFDKPGNA LDHHPTWKHV TCPQCGGKAQ 500
    RETDTMDTFV DSSWYFARFT DPWNENAPTT PAVANRMLPI DQYIGGVEHA 550
    ILHLLYSRFF TRAMKATGHV ALDEPFAGMF TQGMVVHETY QKADGSYVQP 600
    AEVKIELGGN GRRATLLTTG EDIQIGAIEK MSKSKKNTVD PDDIIETYGA 650
    DVARWFMLSD SPPDRDVIWS DERVQGASRF VQRLWRLVND SVELGKAAPA 700
    ARPASFGADA TALRKAAHGA LDKVTTEIER LHFNVCLAHI REFTNAFSEV 750
    LQRPGQPAAD LAWAIREASQ ILVQLFSPMM PHLAEECWQV LGQKGLVSEA 800
    NWPQIERDLL VEDSVTLVVQ VNGKKRGEVT VATAAQNPEI EAAVLALDAV 850
    KLALDGKPVR KVIIVPKRIV NVVG 874
    Length:874
    Mass (Da):97,908
    Last modified:June 20, 2003 - v1
    Checksum:i570F8A8F96A3663B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC45892.1.
    RefSeqiNP_767267.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC45892; BAC45892; BAC45892.
    GeneIDi1049413.
    KEGGibja:blr0627.
    PATRICi21184742. VBIBraJap65052_0645.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC45892.1 .
    RefSeqi NP_767267.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali Q89WQ1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.blr0627.

    Proteomic databases

    PRIDEi Q89WQ1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC45892 ; BAC45892 ; BAC45892 .
    GeneIDi 1049413.
    KEGGi bja:blr0627.
    PATRICi 21184742. VBIBraJap65052_0645.

    Phylogenomic databases

    eggNOGi COG0495.
    HOGENOMi HOG000200747.
    KOi K01869.
    OrthoDBi EOG63Z74X.
    PhylomeDBi Q89WQ1.

    Enzyme and pathway databases

    BioCyci BJAP224911:GJEJ-633-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 3 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_00049_B. Leu_tRNA_synth_B.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002302. Leu-tRNA-ligase.
    IPR025709. Leu_tRNA-synth_edit.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    [Graphical view ]
    PANTHERi PTHR11946:SF7. PTHR11946:SF7. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 2 hits.
    PF13603. tRNA-synt_1_2. 1 hit.
    PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR00985. TRNASYNTHLEU.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00396. leuS_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiSYL_BRADU
    AccessioniPrimary (citable) accession number: Q89WQ1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: June 20, 2003
    Last sequence update: June 20, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3