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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei633 – 6331ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-633-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:blr0627
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 874874Leucine--tRNA ligasePRO_0000151984Add
BLAST

Proteomic databases

PRIDEiQ89WQ1.

Interactioni

Protein-protein interaction databases

STRINGi224911.blr0627.

Structurei

3D structure databases

ProteinModelPortaliQ89WQ1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi43 – 5311"HIGH" regionAdd
BLAST
Motifi630 – 6345"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiQ89WQ1.
KOiK01869.
OrthoDBiEOG63Z74X.
PhylomeDBiQ89WQ1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89WQ1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTSERYNARD SEPRWQAAWD EKAIFVSKND DSRPKYYVLE MFPYPSGRIH
60 70 80 90 100
IGHVRNYTLG DVLARFMRAK GFNVLHPMGW DAFGLPAENA AIERKVAPKA
110 120 130 140 150
WTYDNIAAMK KQLRSIGLSL DWSREIATCD PSYYKHQQKM FLDFLRAGLA
160 170 180 190 200
EREKRKVNWD PVDMTVLANE QVIDGKGWRS GAIVEQREMN QWVFKITKYS
210 220 230 240 250
QELLSALDTL DRWPDKVRLM QRNWIGRSEG LLVRFALDQA TTPAGESELK
260 270 280 290 300
IFTTRPDTLF GAKFMAISAD HPLATAAAAK DPKLAEFIAE IKKIGTAQEI
310 320 330 340 350
IDTAEKQGFD TGIRAIHPFD PSWKLPVYVA NFVLMEYGTG AIFGCPAHDQ
360 370 380 390 400
RDLDFVNKYN LGNTPVVCPE GQDPKSFVIT DTAYDGDGRM INSRFLDGMT
410 420 430 440 450
IEQAKDEVAK RLESELRGNA PVGERQVNFR LRDWGISRQR YWGCPIPVIH
460 470 480 490 500
CPTCDVVPVP DADLPVKLPD DATFDKPGNA LDHHPTWKHV TCPQCGGKAQ
510 520 530 540 550
RETDTMDTFV DSSWYFARFT DPWNENAPTT PAVANRMLPI DQYIGGVEHA
560 570 580 590 600
ILHLLYSRFF TRAMKATGHV ALDEPFAGMF TQGMVVHETY QKADGSYVQP
610 620 630 640 650
AEVKIELGGN GRRATLLTTG EDIQIGAIEK MSKSKKNTVD PDDIIETYGA
660 670 680 690 700
DVARWFMLSD SPPDRDVIWS DERVQGASRF VQRLWRLVND SVELGKAAPA
710 720 730 740 750
ARPASFGADA TALRKAAHGA LDKVTTEIER LHFNVCLAHI REFTNAFSEV
760 770 780 790 800
LQRPGQPAAD LAWAIREASQ ILVQLFSPMM PHLAEECWQV LGQKGLVSEA
810 820 830 840 850
NWPQIERDLL VEDSVTLVVQ VNGKKRGEVT VATAAQNPEI EAAVLALDAV
860 870
KLALDGKPVR KVIIVPKRIV NVVG
Length:874
Mass (Da):97,908
Last modified:June 20, 2003 - v1
Checksum:i570F8A8F96A3663B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC45892.1.
RefSeqiNP_767267.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC45892; BAC45892; BAC45892.
GeneIDi1049413.
KEGGibja:blr0627.
PATRICi21184742. VBIBraJap65052_0645.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC45892.1.
RefSeqiNP_767267.1. NC_004463.1.

3D structure databases

ProteinModelPortaliQ89WQ1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr0627.

Proteomic databases

PRIDEiQ89WQ1.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC45892; BAC45892; BAC45892.
GeneIDi1049413.
KEGGibja:blr0627.
PATRICi21184742. VBIBraJap65052_0645.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
InParanoidiQ89WQ1.
KOiK01869.
OrthoDBiEOG63Z74X.
PhylomeDBiQ89WQ1.

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-633-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiSYL_BRADU
AccessioniPrimary (citable) accession number: Q89WQ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: June 20, 2003
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.