Reviewed, UniProtKB/Swiss-Prot Q89WL0 (GSHB_BRAJA)

Names and origin

Protein names

Recommended name:
    Glutathione synthetase
    EC=6.3.2.3
Alternative name(s):
    Glutathione synthase
    GSH synthetase
      Short name=GSH-S
      Short name=GSHase

Gene names

Name:	gshB
Ordered Locus Names:	bll0668

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP MF_00162
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP MF_00162
Sequence similarities	Belongs to the prokaryotic GSH synthase family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Glutathione biosynthesis
Ligand	ATP-binding Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutathione biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutathione synthase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	Glutathione synthetase HAMAP MF_00162		PRO_0000197456
Regions
	Domain	125 – 309	185	ATP-grasp
	Nucleotide binding	151 – 207	57	ATP By similarity
Sites
	Metal binding	280	1	Magnesium or manganese By similarity
	Metal binding	282	1	Magnesium or manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89WL0-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: AD8FD9B3492CD8B9	FASTA	314	34,760
	10 20 30 40 50 60 MKLNVAVQMD PIARINIKGD STFALLLEAQ KRGHGLSYYT PDKLSMIGEE LVAPVQLLTV 70 80 90 100 110 120 RDEPGDHFTL GEPKREALNG FDVVLLRQDP PFDLAYITST HFLERIHPKT LVVNDPASVR 130 140 150 160 170 180 NAPEKLFVMN FPQLMPPTLI SRDLDEINAF RDKHGAVVMK PLHGHGGAAV FRVMPQDMNF 190 200 210 220 230 240 GSLFDMFTVT FKEPWVIQQF IPEVKHGDKR IILVNGEFAG AVNRVPAADD LRSNMVRGGA 250 260 270 280 290 300 AQETELTPRE REICATVGPA LRERGLLFVG IDVINGNLTE INVTSPTGIR AIARLGGPDV 310 AAKVWDTIEQ KRAK

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC45933.1.
RefSeq	NP_767308.1.
3D structure databases
HSSP	HSSP built from PDB template 1GSH based on UniProtKB P04425.
ModBase	Search...
Genome annotation databases
GeneID	1049304.
GenomeReviews	Gene locus bll0668 in contig BA000040_GR.
KEGG	bja:bll0668.
NMPDR	fig|224911.1.peg.668.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89WL0.
OMA	Q89WL0. VRNAPEK.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0668-MON.
BRENDA	6.3.2.3. 280.
Family and domain databases
HAMAP	MF_00162. [Tree]
InterPro	IPR011761. ATP-grasp. IPR013816. ATP_grasp_subdomain_2. IPR006284. Glut_synth_pro. IPR004218. GSHS_ATP_bd. IPR004215. GSHS_N. IPR013817. Pre-ATP_grasp. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF02955. GSH-S_ATP. 1 hit. PF02951. GSH-S_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01380. glut_syn. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSHB_BRAJA

Accession

Primary (citable) accession number: Q89WL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)