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Q89WL0 (GSHB_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutathione synthetase

EC=6.3.2.3
Alternative name(s):
GSH synthetase
Short name=GSH-S
Short name=GSHase
Glutathione synthase
Gene names
Name:gshB
Ordered Locus Names:bll0668
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length314 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + gamma-L-glutamyl-L-cysteine + glycine = ADP + phosphate + glutathione. HAMAP-Rule MF_00162

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Pathway

Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 2/2. HAMAP-Rule MF_00162

Sequence similarities

Belongs to the prokaryotic GSH synthase family.

Contains 1 ATP-grasp domain.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 314314Glutathione synthetase HAMAP-Rule MF_00162
PRO_0000197456

Regions

Domain125 – 309185ATP-grasp
Nucleotide binding151 – 20757ATP By similarity

Sites

Metal binding2801Magnesium or manganese By similarity
Metal binding2821Magnesium or manganese By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89WL0 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: AD8FD9B3492CD8B9

FASTA31434,760
        10         20         30         40         50         60 
MKLNVAVQMD PIARINIKGD STFALLLEAQ KRGHGLSYYT PDKLSMIGEE LVAPVQLLTV 

        70         80         90        100        110        120 
RDEPGDHFTL GEPKREALNG FDVVLLRQDP PFDLAYITST HFLERIHPKT LVVNDPASVR 

       130        140        150        160        170        180 
NAPEKLFVMN FPQLMPPTLI SRDLDEINAF RDKHGAVVMK PLHGHGGAAV FRVMPQDMNF 

       190        200        210        220        230        240 
GSLFDMFTVT FKEPWVIQQF IPEVKHGDKR IILVNGEFAG AVNRVPAADD LRSNMVRGGA 

       250        260        270        280        290        300 
AQETELTPRE REICATVGPA LRERGLLFVG IDVINGNLTE INVTSPTGIR AIARLGGPDV 

       310 
AAKVWDTIEQ KRAK 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC45933.1.
RefSeqNP_767308.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89WL0.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll0668.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC45933; BAC45933; BAC45933.
GeneID1049304.
KEGGbja:bll0668.
PATRIC21184830. VBIBraJap65052_0689.

Phylogenomic databases

eggNOGCOG0189.
HOGENOMHOG000265022.
KOK01920.
OMAPTCFQEI.
OrthoDBEOG6WMHWB.
ProtClustDBPRK05246.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-674-MONOMER.
UniPathwayUPA00142; UER00210.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00162. GSH_S.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR006284. Glut_synth_pro.
IPR004218. GSHS_ATP-bd.
IPR004215. GSHS_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PfamPF02955. GSH-S_ATP. 1 hit.
PF02951. GSH-S_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01380. glut_syn. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGSHB_BRADU
AccessionPrimary (citable) accession number: Q89WL0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways