Reviewed, UniProtKB/Swiss-Prot Q89WI1 (SYFA_BRAJA)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	bll0706

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity. HAMAP MF_00281
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000126673
Sites
	Metal binding	260	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89WI1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 45C7DD3342D953B3	FASTA	360	40,098
	10 20 30 40 50 60 MSDLATLETS ILDQVAAAGD EAALEAVRVA ALGKKGSISA LLATLGKMSP DDRKTQGAAI 70 80 90 100 110 120 NQAKDKVTEA LAARRDVLKS AALDARLASE TIDVTLPLRE APTDAGRIHP LSQVWDELTT 130 140 150 160 170 180 IFADMGFSVA EGPDIETDDY NFTKLNFPEG HPAREMHDTF FFHPKEDGSR MLLRTHTSPV 190 200 210 220 230 240 QVRTMLSQKP PIRVICPGRT YRIDSDATHT PQFHQVEGLV IDKHSHLGHL KWILHEFCKA 250 260 270 280 290 300 FFEVDHINMR FRPSFFPFTE PSLEVDIQCR RDKGEIRFGE GEDWLEILGC GMVHPNVLRA 310 320 330 340 350 360 CGIDPDEYQG FAWGMGIDRI AMLKYGIADL RQLFDSDVRW LSHYGFKPLE VPTLAGGLSS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC45971.1.
RefSeq	NP_767346.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1049230.
GenomeReviews	Gene locus bll0706 in contig BA000040_GR.
KEGG	bja:bll0706.
NMPDR	fig|224911.1.peg.706.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG284353.
OMA	FRASYFP.
PhylomeDB	Q89WI1.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0706-MONOMER.
BRENDA	6.1.1.20. 280.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR002319. Phenylalanyl-tRNA_Synthase_acu. IPR010978. tRNA_bd_arm. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_BRAJA

Accession

Primary (citable) accession number: Q89WI1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)