Reviewed, UniProtKB/Swiss-Prot Q89WF2 (AROA_BRAJA)

Names and origin

Protein names

Recommended name:
    3-phosphoshikimate 1-carboxyvinyltransferase
    EC=2.5.1.19
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate synthase
      Short name=EPSP synthase
      Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	blr0738

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 469	469	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088230

Sequences

	Sequence		Length	Mass (Da)
	Q89WF2-1 [UniParc]. Last modified June 6, 2003. Version 1. Checksum: DA55AA4F23BB85D5	FASTA	469	48,980
	10 20 30 40 50 60 MPVTGRGPCG TCAPALRSTS KDTILTHSDQ PRPLQSRANG PLTGKVRVPG DKSISHRALI 70 80 90 100 110 120 LGALAVGETR ISGLLEGEDV LNTAKSMQAL GASVERTGDF AWKVQGVGVA GFAQPKAALD 130 140 150 160 170 180 FGNSGTGCRL VMGAVAGCPI SAVFDGDASL RSRPMRRILD PLEKMGARVV SGGEGGRLPL 190 200 210 220 230 240 TLQGARDPLP ITYKTPVASA QIKSAVLLAG LAAPGTTTVI ESEASRDHTE LMLKHFGADI 250 260 270 280 290 300 TSTKEGQHGR RITLVGQPEL HGANVVVPAD PSSAAFPVVA ALIAEGSDVV LSDVMTNPLR 310 320 330 340 350 360 TGLFTTLREM GASIEESEVR GDAGEPMAQL RVRASKLRGV EVPPERAPSM IDEYLVLAVA 370 380 390 400 410 420 ASFAEGTTIM RGLQELRVKE SDRLEATAEM LRVNGVKVEV SGDDLVVQGR GHVPGGGTVA 430 440 450 460 THMDHRIAMS ALVMGCASDQ PVTVDDTAFI ATSFPDFIPM MRSLGAEFS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC46003.1.
RefSeq	NP_767378.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1049147.
GenomeReviews	Gene locus blr0738 in contig BA000040_GR.
KEGG	bja:blr0738.
NMPDR	fig|224911.1.peg.738.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89WF2.
OMA	DPLELMG.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR0738-MON.
BRENDA	2.5.1.19. 280.
Family and domain databases
HAMAP	MF_00210. [Tree]
InterPro	IPR016228. EPSP_synthase. IPR001986. EPSP_synthase_core. IPR006264. EPSP_synthase_subgroup. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 1 hit.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
ProDom	PD001867. EPSP_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01356. aroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_BRAJA

Accession

Primary (citable) accession number: Q89WF2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	June 6, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)