Reviewed, UniProtKB/Swiss-Prot Q89WB4 (KATG_BRAJA)

Names and origin

Protein names

Recommended name:
    Catalase-peroxidase
      Short name=CP
    EC=1.11.1.6
    EC=1.11.1.7
Alternative name(s):
    Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	blr0778

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	779 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity.
Catalytic activity	2 H2O2 = O2 + 2 H2O. HAMAP MF_01961 Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity.
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity.
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: HAMAP heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 779	779	Catalase-peroxidase HAMAP MF_01961		PRO_0000354727
Sites
	Active site	149	1	Proton acceptor By similarity
	Metal binding	311	1	Iron (heme axial ligand) By similarity
	Site	145	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	148 ↔ 270		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-296) By similarity
	Cross-link	270 ↔ 296		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-148) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89WB4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7BF99C75572BB055	FASTA	779	85,664
	10 20 30 40 50 60 MAVTRQRVEI PELAHRHVDH QKILSQRSLK STSPDGMRGL EWFYLVKGAS STPPEEVFMD 70 80 90 100 110 120 DTSKCPFSGG KRVPANRDWW PTQLSIEMLH KNSGLSDPMG EEFDYAKEFK TLDLNAVIKD 130 140 150 160 170 180 LTALMTDSQE WWPADFGHYG GLMIRMAWHS AGTYRITDGR GGAGAGQQRF APLNSWPDNA 190 200 210 220 230 240 NLDKARRLLW PIKQKYGRKI SWADLMVLAG NVALESMGFK TFGFAGGRAD VWEPEELYWG 250 260 270 280 290 300 PEGTWLGDER YSGERQLAEP LGAVQMGLIY VNPEGPNGKP DPIAAAKDIR ETFFRMAMND 310 320 330 340 350 360 EETVALIAGG HTFGKTHGAG DPSLVGPEPE AGALEDQGLG WKSKHASGLA GDSITSGLEV 370 380 390 400 410 420 TWTTTPTKWS NNFFENLFKY EWELTKSPGG ANQWTAKGAE AIIPDAFDKS KKHRPTMLTT 430 440 450 460 470 480 DLSLRLDPAY EKISRRFLEN PDQFADAFAR AWFKLTHRDM GPIQRYLGPL VPKETLIWQD 490 500 510 520 530 540 PIPAVNHELV SDQDIAALKT KILASGLSVP ELVSTAWASA STFRGSDKRG GANGARIRLS 550 560 570 580 590 600 PQKDWEVNQP AQLSKVLGKL EAIQKEFNAS AGAKKVSLAD LIVLGGTAAV EKAAKDAGVD 610 620 630 640 650 660 VKVAFTPGRM DASQEQTDAA SFAPLEPRAD GFRNYVGKRQ QFLQQEEALV DRAQLLKLTG 670 680 690 700 710 720 PELTVLVGGL RVLGANANGS KHGVLTAKVG ALSNDFFVNL LDMSTQWAPA ADGTYEARDR 730 740 750 760 770 KTNAVKWTGT RADLIFGAHS QLRAYAEVYA TSDSKEQFVK DFAKAWTKVM NLDRFDLAA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC46043.1.
RefSeq	NP_767418.1.
3D structure databases
HSSP	HSSP built from PDB template 1MWV based on UniProtKB Q939D2.
ModBase	Search...
Protein family/group databases
PeroxiBase	2347. BjaCP01_USDA110.
Genome annotation databases
GeneID	1049955.
GenomeReviews	Gene locus blr0778 in contig BA000040_GR.
KEGG	bja:blr0778.
NMPDR	fig|224911.1.peg.778.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89WB4.
OMA	FEWELTK.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR0778-MON.
Family and domain databases
HAMAP	MF_01961. [Tree]
InterPro	IPR000763. Catalase_proxase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
TIGRFAMs	TIGR00198. cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_BRAJA

Accession

Primary (citable) accession number: Q89WB4

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)