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Q89WB3

- PNP_BRADU

UniProt

Q89WB3 - PNP_BRADU

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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi487 – 4871MagnesiumUniRule annotation
Metal bindingi493 – 4931MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-787-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:bll0779
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 720720Polyribonucleotide nucleotidyltransferasePRO_0000329542Add
BLAST

Proteomic databases

PRIDEiQ89WB3.

Interactioni

Protein-protein interaction databases

STRINGi224911.bll0779.

Structurei

3D structure databases

ProteinModelPortaliQ89WB3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini554 – 61360KHUniRule annotationAdd
BLAST
Domaini623 – 69169S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
InParanoidiQ89WB3.
KOiK00962.
OrthoDBiEOG6WT8CC.
PhylomeDBiQ89WB3.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89WB3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MFNKHSVEID WGGRPLKLET GKIARQADGA VVATYGETVV LATVVAAKAP
60 70 80 90 100
REGVDFLPLT VDYQEKTYAA GRIPGGYFKR EGRPTEKETL VSRLIDRPIR
110 120 130 140 150
PLFVDGWRNE TQVIATVLSH DMENDPDIVA LVASSAALTL SGAPFKGPIG
160 170 180 190 200
AARVGFVNDE YVLNPTLDEM VDTQLDLVVA GTADAVLMVE SEAKELNEDI
210 220 230 240 250
MLGAVMFGHR HFQPVINAII ELAEKAAKEP REVTVIDNAA LEKEMLGLVE
260 270 280 290 300
QELRAAYAIP VKQDRYAAVG KVKEKVIAHY FPEGQEPKYD KLRIAGVFKE
310 320 330 340 350
LEAKIVRWNI LDTGKRIDGR DSKTVRNIIA EVGVLPRAHG SALFTRGETQ
360 370 380 390 400
AMVVTTLGTG EDEQYIDALS GTYKETFLLH YNFPPYSVGE TGRLGGTKRR
410 420 430 440 450
EIGHGKLAWR AIHPVLPPHH EFPYTIRVVS EITESNGSSS MASVCGASLA
460 470 480 490 500
LMDAGVPLKR PTAGIAMGLI LEDKRFAVLS DILGDEDHLG DMDFKVAGTE
510 520 530 540 550
SGITSLQMDI KIEGITEEIM KVALGQAKDG RIHILGEMAK ALTNARAELG
560 570 580 590 600
EYAPRIETFK IPTDKIREVI GTGGKVIREI VEKTGAKVNI EDDGTVKVAS
610 620 630 640 650
SDGEAMKAAI KWIKSIASEP EVGQIYDGTV VKVMEFGAFV NFFGSKDGLV
660 670 680 690 700
HISQLASARV QKTSDVVKEG DKVKVKLLGF DDRGKTRLSM KVVDQTTGED
710 720
LEAKDKVAEG EKAPREAAGE
Length:720
Mass (Da):78,299
Last modified:June 1, 2003 - v1
Checksum:i12FB5FDE3A440DB2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46044.1.
RefSeqiNP_767419.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46044; BAC46044; BAC46044.
GeneIDi1048800.
KEGGibja:bll0779.
PATRICi21185062. VBIBraJap65052_0803.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46044.1 .
RefSeqi NP_767419.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89WB3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.bll0779.

Proteomic databases

PRIDEi Q89WB3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC46044 ; BAC46044 ; BAC46044 .
GeneIDi 1048800.
KEGGi bja:bll0779.
PATRICi 21185062. VBIBraJap65052_0803.

Phylogenomic databases

eggNOGi COG1185.
HOGENOMi HOG000218327.
InParanoidi Q89WB3.
KOi K00962.
OrthoDBi EOG6WT8CC.
PhylomeDBi Q89WB3.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-787-MONOMER.

Family and domain databases

Gene3Di 1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPi MF_01595. PNPase.
InterProi IPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view ]
PANTHERi PTHR11252. PTHR11252. 1 hit.
Pfami PF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view ]
PIRSFi PIRSF005499. PNPase. 1 hit.
SMARTi SM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view ]
SUPFAMi SSF46915. SSF46915. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
PROSITEi PS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiPNP_BRADU
AccessioniPrimary (citable) accession number: Q89WB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 29, 2008
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3