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Q89WB3

- PNP_BRADU

UniProt

Q89WB3 - PNP_BRADU

Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 73 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

    Catalytic activityi

    RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi487 – 4871MagnesiumUniRule annotation
    Metal bindingi493 – 4931MagnesiumUniRule annotation

    GO - Molecular functioni

    1. 3'-5'-exoribonuclease activity Source: InterPro
    2. magnesium ion binding Source: UniProtKB-HAMAP
    3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
    4. RNA binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. mRNA catabolic process Source: UniProtKB-HAMAP
    2. RNA processing Source: InterPro

    Keywords - Molecular functioni

    Nucleotidyltransferase, Transferase

    Keywords - Ligandi

    Magnesium, Metal-binding, RNA-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-787-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
    Alternative name(s):
    Polynucleotide phosphorylaseUniRule annotation
    Short name:
    PNPaseUniRule annotation
    Gene namesi
    Name:pnpUniRule annotation
    Ordered Locus Names:bll0779
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 720720Polyribonucleotide nucleotidyltransferasePRO_0000329542Add
    BLAST

    Proteomic databases

    PRIDEiQ89WB3.

    Interactioni

    Protein-protein interaction databases

    STRINGi224911.bll0779.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89WB3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini554 – 61360KHUniRule annotationAdd
    BLAST
    Domaini623 – 69169S1 motifUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
    Contains 1 KH domain.UniRule annotation
    Contains 1 S1 motif domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG1185.
    HOGENOMiHOG000218327.
    KOiK00962.
    OrthoDBiEOG6WT8CC.
    PhylomeDBiQ89WB3.

    Family and domain databases

    Gene3Di1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPiMF_01595. PNPase.
    InterProiIPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view]
    PANTHERiPTHR11252. PTHR11252. 1 hit.
    PfamiPF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005499. PNPase. 1 hit.
    SMARTiSM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view]
    SUPFAMiSSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
    PROSITEiPS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q89WB3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFNKHSVEID WGGRPLKLET GKIARQADGA VVATYGETVV LATVVAAKAP    50
    REGVDFLPLT VDYQEKTYAA GRIPGGYFKR EGRPTEKETL VSRLIDRPIR 100
    PLFVDGWRNE TQVIATVLSH DMENDPDIVA LVASSAALTL SGAPFKGPIG 150
    AARVGFVNDE YVLNPTLDEM VDTQLDLVVA GTADAVLMVE SEAKELNEDI 200
    MLGAVMFGHR HFQPVINAII ELAEKAAKEP REVTVIDNAA LEKEMLGLVE 250
    QELRAAYAIP VKQDRYAAVG KVKEKVIAHY FPEGQEPKYD KLRIAGVFKE 300
    LEAKIVRWNI LDTGKRIDGR DSKTVRNIIA EVGVLPRAHG SALFTRGETQ 350
    AMVVTTLGTG EDEQYIDALS GTYKETFLLH YNFPPYSVGE TGRLGGTKRR 400
    EIGHGKLAWR AIHPVLPPHH EFPYTIRVVS EITESNGSSS MASVCGASLA 450
    LMDAGVPLKR PTAGIAMGLI LEDKRFAVLS DILGDEDHLG DMDFKVAGTE 500
    SGITSLQMDI KIEGITEEIM KVALGQAKDG RIHILGEMAK ALTNARAELG 550
    EYAPRIETFK IPTDKIREVI GTGGKVIREI VEKTGAKVNI EDDGTVKVAS 600
    SDGEAMKAAI KWIKSIASEP EVGQIYDGTV VKVMEFGAFV NFFGSKDGLV 650
    HISQLASARV QKTSDVVKEG DKVKVKLLGF DDRGKTRLSM KVVDQTTGED 700
    LEAKDKVAEG EKAPREAAGE 720
    Length:720
    Mass (Da):78,299
    Last modified:June 1, 2003 - v1
    Checksum:i12FB5FDE3A440DB2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC46044.1.
    RefSeqiNP_767419.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC46044; BAC46044; BAC46044.
    GeneIDi1048800.
    KEGGibja:bll0779.
    PATRICi21185062. VBIBraJap65052_0803.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC46044.1 .
    RefSeqi NP_767419.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali Q89WB3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.bll0779.

    Proteomic databases

    PRIDEi Q89WB3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC46044 ; BAC46044 ; BAC46044 .
    GeneIDi 1048800.
    KEGGi bja:bll0779.
    PATRICi 21185062. VBIBraJap65052_0803.

    Phylogenomic databases

    eggNOGi COG1185.
    HOGENOMi HOG000218327.
    KOi K00962.
    OrthoDBi EOG6WT8CC.
    PhylomeDBi Q89WB3.

    Enzyme and pathway databases

    BioCyci BJAP224911:GJEJ-787-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.400. 1 hit.
    2.40.50.140. 1 hit.
    3.30.1370.10. 1 hit.
    3.30.230.70. 2 hits.
    HAMAPi MF_01595. PNPase.
    InterProi IPR001247. ExoRNase_PH_dom1.
    IPR015847. ExoRNase_PH_dom2.
    IPR004087. KH_dom.
    IPR004088. KH_dom_type_1.
    IPR012340. NA-bd_OB-fold.
    IPR012162. PNPase.
    IPR027408. PNPase/RNase_PH_dom.
    IPR015848. PNPase_PH_RNA-bd_bac/org-type.
    IPR003029. Rbsml_prot_S1_RNA-bd_dom.
    IPR020568. Ribosomal_S5_D2-typ_fold.
    IPR022967. RNA-binding_domain_S1.
    [Graphical view ]
    PANTHERi PTHR11252. PTHR11252. 1 hit.
    Pfami PF00013. KH_1. 1 hit.
    PF03726. PNPase. 1 hit.
    PF01138. RNase_PH. 2 hits.
    PF03725. RNase_PH_C. 2 hits.
    PF00575. S1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005499. PNPase. 1 hit.
    SMARTi SM00322. KH. 1 hit.
    SM00316. S1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46915. SSF46915. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF54211. SSF54211. 2 hits.
    SSF54791. SSF54791. 1 hit.
    SSF55666. SSF55666. 2 hits.
    TIGRFAMsi TIGR03591. polynuc_phos. 1 hit.
    PROSITEi PS50084. KH_TYPE_1. 1 hit.
    PS50126. S1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiPNP_BRADU
    AccessioniPrimary (citable) accession number: Q89WB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 29, 2008
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 73 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3