Reviewed, UniProtKB/Swiss-Prot Q89WB3 (PNP_BRAJA)

Names and origin

Protein names

Recommended name:
    Polyribonucleotide nucleotidyltransferase
    EC=2.7.7.8
Alternative name(s):
    Polynucleotide phosphorylase
      Short name=PNPase

Gene names

Name:	pnp
Ordered Locus Names:	bll0779

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	720 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction By similarity.
Catalytic activity	RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate. HAMAP MF_01595
Subunit structure	Homotrimer. Organized into a structure (processome or RNA degradosome) containing a number of RNA-processing enzymes By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the polyribonucleotide nucleotidyltransferase family. Contains 1 KH domain. Contains 1 S1 motif domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	RNA-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	RNA processing Inferred from electronic annotation. Source: InterPro mRNA catabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5'-exoribonuclease activity Inferred from electronic annotation. Source: InterPro RNA binding Inferred from electronic annotation. Source: UniProtKB-KW polyribonucleotide nucleotidyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 720	720	Polyribonucleotide nucleotidyltransferase HAMAP MF_01595		PRO_0000329542
Regions
	Domain	554 – 613	60	KH
	Domain	623 – 691	69	S1 motif

Sequences

	Sequence		Length	Mass (Da)
	Q89WB3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 12FB5FDE3A440DB2	FASTA	720	78,299
	10 20 30 40 50 60 MFNKHSVEID WGGRPLKLET GKIARQADGA VVATYGETVV LATVVAAKAP REGVDFLPLT 70 80 90 100 110 120 VDYQEKTYAA GRIPGGYFKR EGRPTEKETL VSRLIDRPIR PLFVDGWRNE TQVIATVLSH 130 140 150 160 170 180 DMENDPDIVA LVASSAALTL SGAPFKGPIG AARVGFVNDE YVLNPTLDEM VDTQLDLVVA 190 200 210 220 230 240 GTADAVLMVE SEAKELNEDI MLGAVMFGHR HFQPVINAII ELAEKAAKEP REVTVIDNAA 250 260 270 280 290 300 LEKEMLGLVE QELRAAYAIP VKQDRYAAVG KVKEKVIAHY FPEGQEPKYD KLRIAGVFKE 310 320 330 340 350 360 LEAKIVRWNI LDTGKRIDGR DSKTVRNIIA EVGVLPRAHG SALFTRGETQ AMVVTTLGTG 370 380 390 400 410 420 EDEQYIDALS GTYKETFLLH YNFPPYSVGE TGRLGGTKRR EIGHGKLAWR AIHPVLPPHH 430 440 450 460 470 480 EFPYTIRVVS EITESNGSSS MASVCGASLA LMDAGVPLKR PTAGIAMGLI LEDKRFAVLS 490 500 510 520 530 540 DILGDEDHLG DMDFKVAGTE SGITSLQMDI KIEGITEEIM KVALGQAKDG RIHILGEMAK 550 560 570 580 590 600 ALTNARAELG EYAPRIETFK IPTDKIREVI GTGGKVIREI VEKTGAKVNI EDDGTVKVAS 610 620 630 640 650 660 SDGEAMKAAI KWIKSIASEP EVGQIYDGTV VKVMEFGAFV NFFGSKDGLV HISQLASARV 670 680 690 700 710 720 QKTSDVVKEG DKVKVKLLGF DDRGKTRLSM KVVDQTTGED LEAKDKVAEG EKAPREAAGE

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC46044.1.
RefSeq	NP_767419.1.
3D structure databases
HSSP	HSSP built from PDB template 1SRO based on UniProtKB P05055.
ModBase	Search...
Genome annotation databases
GeneID	1048800.
GenomeReviews	Gene locus bll0779 in contig BA000040_GR.
KEGG	bja:bll0779.
NMPDR	fig|224911.1.peg.779.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89WB3.
OMA	Q89WB3. GHGNLAK.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0779-MON.
Family and domain databases
HAMAP	MF_01595. [Tree]
InterPro	IPR001247. ExoRNase_PH_dom1. IPR015847. ExoRNase_PH_dom2. IPR004087. KH. IPR004088. KH_type_1. IPR018111. KH_type_1_subgr. IPR012340. NA-bd_OB-fold. IPR012162. PNPase. IPR015848. PNPase_PH_RNA-bd_bac/org-type. IPR003029. Rbsml_prot_S1_RNA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit. G3DSA:1.10.10.400. PNPase_PH_RNA-bd_bac/org-type. 1 hit.
PANTHER	PTHR11252. PNPase. 1 hit.
Pfam	PF00013. KH_1. 1 hit. PF03726. PNPase. 1 hit. PF01138. RNase_PH. 2 hits. PF03725. RNase_PH_C. 2 hits. PF00575. S1. 1 hit. [Graphical view]
PIRSF	PIRSF005499. PNPase. 1 hit.
SMART	SM00322. KH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03591. Polynuc_phos. 1 hit.
PROSITE	PS50084. KH_TYPE_1. 1 hit. PS50126. S1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PNP_BRAJA

Accession

Primary (citable) accession number: Q89WB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)