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Q89WA4

- TRMB_BRADU

UniProt

Q89WA4 - TRMB_BRADU

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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene
trmB, bll0788
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 2 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei67 – 671S-adenosyl-L-methionine By similarity
Binding sitei92 – 921S-adenosyl-L-methionine By similarity
Binding sitei119 – 1191S-adenosyl-L-methionine By similarity
Active sitei141 – 1411 By similarity
Binding sitei141 – 1411S-adenosyl-L-methionine By similarity
Binding sitei145 – 1451Substrate By similarity
Binding sitei177 – 1771Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-796-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:bll0788
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236tRNA (guanine-N(7)-)-methyltransferaseUniRule annotationPRO_0000171304Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.bll0788.

Structurei

3D structure databases

ProteinModelPortaliQ89WA4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000073969.
KOiK03439.
OrthoDBiEOG6K6VBC.
PhylomeDBiQ89WA4.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89WA4-1 [UniParc]FASTAAdd to Basket

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MSERKSDPDR DDSERAFFGR RKGHKLRQHQ AELIDHLLPH LALDIAGEAP    50
GNACEIFDPA ASDVRLEIGF GGGEHLAAEA QNFATTGFIG CEPYVNGMAK 100
ILAQIEAGNI ANIRLFAGDA AELLAWLPKA SLSRIDLIHP DPWPKRRHWK 150
RRFVQDRTIA AMARVLKVGG EFRFVCDIDD YCAWTLSHLA RSADFKWLAE 200
RADDFRQPWD GYTMTRYGRK AAREGRKAAY LRFRRS 236
Length:236
Mass (Da):26,766
Last modified:June 16, 2003 - v1
Checksum:i09D1B7A43966F01C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC46053.1.
RefSeqiNP_767428.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46053; BAC46053; BAC46053.
GeneIDi1051109.
KEGGibja:bll0788.
PATRICi21185080. VBIBraJap65052_0812.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC46053.1 .
RefSeqi NP_767428.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89WA4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.bll0788.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC46053 ; BAC46053 ; BAC46053 .
GeneIDi 1051109.
KEGGi bja:bll0788.
PATRICi 21185080. VBIBraJap65052_0812.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000073969.
KOi K03439.
OrthoDBi EOG6K6VBC.
PhylomeDBi Q89WA4.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci BJAP224911:GJEJ-796-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiTRMB_BRADU
AccessioniPrimary (citable) accession number: Q89WA4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: July 9, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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