Q89WA4 (TRMB_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	bll0788

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	236 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA. HAMAP MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 236	236	tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057		PRO_0000171304
Sites
	Binding site	67	1	S-adenosyl-L-methionine By similarity
	Binding site	92	1	S-adenosyl-L-methionine By similarity
	Binding site	119	1	S-adenosyl-L-methionine By similarity
	Binding site	141	1	S-adenosyl-L-methionine By similarity
	Binding site	145	1	Substrate By similarity
	Binding site	177	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89WA4 [UniParc]. Last modified June 16, 2003. Version 1. Checksum: 09D1B7A43966F01C	FASTA	236	26,766
	10 20 30 40 50 60 MSERKSDPDR DDSERAFFGR RKGHKLRQHQ AELIDHLLPH LALDIAGEAP GNACEIFDPA 70 80 90 100 110 120 ASDVRLEIGF GGGEHLAAEA QNFATTGFIG CEPYVNGMAK ILAQIEAGNI ANIRLFAGDA 130 140 150 160 170 180 AELLAWLPKA SLSRIDLIHP DPWPKRRHWK RRFVQDRTIA AMARVLKVGG EFRFVCDIDD 190 200 210 220 230 YCAWTLSHLA RSADFKWLAE RADDFRQPWD GYTMTRYGRK AAREGRKAAY LRFRRS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC46053.1.
RefSeq	NP_767428.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89WA4.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1051109.
GenomeReviews	Gene locus bll0788 in contig BA000040_GR.
KEGG	bja:bll0788.
NMPDR	fig|224911.1.peg.788.
PATRIC	21185080. VBIBraJap65052_0812.
Phylogenomic databases
HOGENOM	HBG322782.
OMA	LYPDPWP.
PhylomeDB	Q89WA4.
ProtClustDB	PRK00121.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0788-MONOMER.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB. [Tree]
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
KO	K03439.
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMB_BRAJA

Accession

Primary (citable) accession number: Q89WA4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 16, 2003
Last sequence update:	June 16, 2003
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program