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Reviewed, UniProtKB/Swiss-Prot Q89WA4 (TRMB_BRAJA)

Last modified June 16, 2009. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    tRNA (guanine-N(7)-)-methyltransferase
    EC=2.1.1.33
Alternative name(s):
    tRNA(m7G46)-methyltransferase
Gene names
Name: trmB
Ordered Locus Names: bll0788
OrganismBradyrhizobium japonicum [Complete proteome] [HAMAP]
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

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Protein attributes

Sequence length236 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.

Catalytic activity

S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing N(7)-methylguanine. HAMAP MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP MF_01057

Sequence similarities

Belongs to the methyltransferase superfamily. TrmB family.

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Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processtRNA modification

Inferred from electronic annotation. Source: HAMAP

   Molecular functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 236236tRNA (guanine-N(7)-)-methyltransferase HAMAP MF_01057
PRO_0000171304

Sites

Binding site671S-adenosyl-L-methionine By similarity
Binding site921S-adenosyl-L-methionine By similarity
Binding site1191S-adenosyl-L-methionine By similarity
Binding site1411S-adenosyl-L-methionine By similarity
Binding site1451Substrate By similarity
Binding site1771Substrate Potential

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Sequences

Sequence LengthMass (Da)Tools
Q89WA4-1 [UniParc].

Last modified June 16, 2003. Version 1.
Checksum: 09D1B7A43966F01C

FASTA23626,766
        10         20         30         40         50         60 
MSERKSDPDR DDSERAFFGR RKGHKLRQHQ AELIDHLLPH LALDIAGEAP GNACEIFDPA 

        70         80         90        100        110        120 
ASDVRLEIGF GGGEHLAAEA QNFATTGFIG CEPYVNGMAK ILAQIEAGNI ANIRLFAGDA 

       130        140        150        160        170        180 
AELLAWLPKA SLSRIDLIHP DPWPKRRHWK RRFVQDRTIA AMARVLKVGG EFRFVCDIDD 

       190        200        210        220        230 
YCAWTLSHLA RSADFKWLAE RADDFRQPWD GYTMTRYGRK AAREGRKAAY LRFRRS

« Hide

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References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

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Cross-references

Sequence databases

BA000040 Genomic DNA. Translation: BAC46053.1.
RefSeqNP_767428.1.

3D structure databases

ModBaseSearch...

Genome annotation databases

GeneID1051109.
GenomeReviewsGene locus bll0788 in contig BA000040_GR.
KEGGbja:bll0788.
NMPDRfig|224911.1.peg.788.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMQ89WA4.
OMAQ89WA4. YPDPWPK.

Enzyme and pathway databases

BioCycBJAP224911:BLL0788-MON.
BRENDA2.1.1.33. 280.

Family and domain databases

HAMAPMF_01057.
[Tree]
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PANTHERPTHR23417:SF1. Methyltransf_4. 1 hit.
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameTRMB_BRAJA
AccessionPrimary (citable) accession number: Q89WA4
Entry history
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: June 16, 2003
Last modified: June 16, 2009
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents