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Protein

Tryptophan--tRNA ligase

Gene

trpS

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of tryptophan to tRNA(Trp).UniRule annotation

Catalytic activityi

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei140L-tryptophanUniRule annotation1
Binding sitei202ATP; via amide nitrogen and carbonyl oxygenUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 14ATPUniRule annotation3
Nucleotide bindingi20 – 21ATPUniRule annotation2
Nucleotide bindingi152 – 154ATPUniRule annotation3
Nucleotide bindingi211 – 215ATPUniRule annotation5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBDIA224911:G1G3J-810-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan--tRNA ligaseUniRule annotation (EC:6.1.1.2UniRule annotation)
Alternative name(s):
Tryptophanyl-tRNA synthetaseUniRule annotation
Short name:
TrpRSUniRule annotation
Gene namesi
Name:trpSUniRule annotation
Ordered Locus Names:bll0802
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001366071 – 350Tryptophan--tRNA ligaseAdd BLAST350

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi224911.bll0802

Structurei

3D structure databases

ProteinModelPortaliQ89W91
SMRiQ89W91
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi13 – 21"HIGH" regionUniRule annotation9
Motifi211 – 215"KMSKS" regionUniRule annotation5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C31 Bacteria
COG0180 LUCA
HOGENOMiHOG000059940
InParanoidiQ89W91
KOiK01867
OMAiQRMTQFK
OrthoDBiPOG091H00I9
PhylomeDBiQ89W91

Family and domain databases

CDDicd00806 TrpRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_00140_B Trp_tRNA_synth_B, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002305 aa-tRNA-synth_Ic
IPR014729 Rossmann-like_a/b/a_fold
IPR002306 Trp-tRNA-ligase
IPR024109 Trp-tRNA-ligase_bac-type
PfamiView protein in Pfam
PF00579 tRNA-synt_1b, 1 hit
PRINTSiPR01039 TRNASYNTHTRP
TIGRFAMsiTIGR00233 trpS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q89W91-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFVERVFSG VQPTGNLHLG NYLGAIVNFV KMQETHNCIY CVVDMHAITQ
60 70 80 90 100
GVDVWGGPVE LARNTREVTA AFIASGIDPK KHIVFNQSQV SGHAELAWIF
110 120 130 140 150
NCVARMGWLG RMTQFKEKAG KDRENASVGL FDYPVLMAAD ILLYRATHVP
160 170 180 190 200
VGEDQKQHLE LSRDIAQKFN NDFGDSIRAQ GTNDGLFFPL PEPFITGPAT
210 220 230 240 250
RVMSLRDGTK KMSKSDASDN SRINLTDDAD TIAQKIRKAK TDPEPLPTEE
260 270 280 290 300
KGLEARPEAD NLVGIFAALA DRSKADVLRD FGGGQFSSFK NALAELCVTK
310 320 330 340 350
LAPIAGEMKR LVADPGHIDA ILNEGSDRAR AIADETMKLS KDIVGFIRRR
Length:350
Mass (Da):38,472
Last modified:June 1, 2003 - v1
Checksum:i210D214CC4F58990
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA Translation: BAC46067.1
RefSeqiNP_767442.1, NC_004463.1
WP_011083624.1, NZ_CP011360.1

Genome annotation databases

EnsemblBacteriaiBAC46067; BAC46067; BAC46067
GeneIDi1048862
KEGGibja:bll0802
PATRICifig|224911.44.peg.178

Similar proteinsi

Entry informationi

Entry nameiSYW_BRADU
AccessioniPrimary (citable) accession number: Q89W91
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: March 28, 2018
This is version 83 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health