Reviewed, UniProtKB/Swiss-Prot Q89W81 (PUR72_BRAJA)

Names and origin

Protein names

Recommended name:
    Putative phosphoribosylaminoimidazole-succinocarboxamide synthase B
    EC=6.3.2.6
Alternative name(s):
    SAICAR synthetase 2

Gene names

Name:	purC2
Ordered Locus Names:	bll0812

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate. HAMAP MF_00137
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 4/5. HAMAP MF_00137
Sequence similarities	Belongs to the SAICAR synthetase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Putative phosphoribosylaminoimidazole-succinocarboxamide synthase B HAMAP MF_00137		PRO_0000100808

Sequences

	Sequence		Length	Mass (Da)
	Q89W81-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: EA918E87E17BCB46	FASTA	304	33,701
	10 20 30 40 50 60 MTTMLSSDLP LPKIGRGKVR DIYAVDDDRL LLLTTDRISA FDVVMGETIP MKGAVLTQIS 70 80 90 100 110 120 AFWFGELEGV VPHHMISADT DEIIAAVPAL KPHRAEILGR AMLSRRTTVF PIECVIRGYL 130 140 150 160 170 180 SGSAWKEYAT SGTLAGEKLK AGLVESEKLE PAIFSPATKA ETGHDENITI ARMREVVGDE 190 200 210 220 230 240 TAYTLESMTR AIYTLGEELA REQGIIIADT KFEFGRDKDG RIILIDEVMT PDSSRFWAVD 250 260 270 280 290 300 AYKPGQPQAS FDKQPLRDYL DVERRAGRWN GDAPPPPLPA SVVEATSKRY LEAYRRVTGK ELKI

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC46077.1.
RefSeq	NP_767452.1.
3D structure databases
HSSP	HSSP built from PDB template 1OBD based on UniProtKB P27616.
ModBase	Search...
Genome annotation databases
GeneID	1051356.
GenomeReviews	Gene locus bll0812 in contig BA000040_GR.
KEGG	bja:bll0812.
NMPDR	fig|224911.1.peg.812.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89W81.
OMA	Q89W81. LTLMDEV.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL0812-MON.
BRENDA	6.3.2.6. 280.
Family and domain databases
HAMAP	MF_00137. [Tree]
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR001636. SAICAR_synt. IPR018236. SAICAR_synthetase_CS. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
PANTHER	PTHR11609. SAICAR_synt. 1 hit.
Pfam	PF01259. SAICAR_synt. 1 hit. [Graphical view]
ProDom	PD003043. SAICAR_synt. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00081. purC. 1 hit.
PROSITE	PS01057. SAICAR_SYNTHETASE_1. 1 hit. PS01058. SAICAR_SYNTHETASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR72_BRAJA

Accession

Primary (citable) accession number: Q89W81

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)