Q89VX9 (GLND_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
[Protein-PII] uridylyltransferase
Short name=PII uridylyl-transferase
EC=2.7.7.59

Alternative name(s):
UTase
Uridylyl-removing enzyme

Gene names

Name:	glnD
Ordered Locus Names:	bll0916

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium japonicum

Protein attributes

Sequence length	929 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Modifies, by uridylylation or deuridylylation the PII (GlnB) regulatory protein By similarity. HAMAP-Rule MF_00277
Catalytic activity	UTP + [protein-PII] = diphosphate + uridylyl-[protein-PII]. HAMAP-Rule MF_00277
Sequence similarities	Belongs to the GlnD family.
Sequence caution	The sequence BAC46181.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Nitrogen fixation
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: HAMAP
Molecular_function	[protein-PII] uridylyltransferase activity Inferred from electronic annotation. Source: HAMAP amino acid binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: InterPro phosphoric diester hydrolase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 929	929	[Protein-PII] uridylyltransferase HAMAP-Rule MF_00277		PRO_0000192721

Sequences

	Sequence		Length	Mass (Da)
	Q89VX9 [UniParc]. Last modified July 5, 2004. Version 2. Checksum: E4A270C497C26D5E	FASTA	929	105,033
	10 20 30 40 50 60 MDSVTTEHKQ EVDDRFDTAR ITAAVDALAE KHQGREDAFR TAMAQLLKAE LIAARAAAQA 70 80 90 100 110 120 ILLKDRHGRR CAERLCHVQD EIIRILYSAA TRHLYRSPIP SGAERMAVVA TGGYGRGLMA 130 140 150 160 170 180 PESDIDLLFI LPYKQTAWGE QVAEAILYCL WDMGLKVGHA TRSVDESIRQ ARGDMTIRTA 190 200 210 220 230 240 ILETRFLTGD QPLYDELVER FDKEVVQGTA SEFVTAKLAE REERHRRGGQ SRYLVEPNVK 250 260 270 280 290 300 DGKGALRDLH TLFWIAKYVY RVRDTDELVE RGVFDAQEYR TFRRCADFLW SVRCNLHFYS 310 320 330 340 350 360 GRAEERLSFD LQREIAVRLG YTSHPGMQDV ERFMKHYFLV AKEVGNLTAI LCAKLEDQQA 370 380 390 400 410 420 KPAPVLSRMM ARLRPTPAKR RVPDSDDFIV DNNRINVAAP DVFKHDPVNL IRIFRLAQKH 430 440 450 460 470 480 NLAFHPDAMR DVTRSLGLIN AQLRENPEAN RLFMEILTSD NAEIVLRRMN ETGVLGHFIR 490 500 510 520 530 540 AFGKIVSMMQ FNMYHHYTVD EHLIRCVGFL QDIERGGIEE FAVASDLMRK IRPEHRSVIY 550 560 570 580 590 600 IATLLHDVAK GRPEDHSIAG AKVARRLCPR LGFSPADTEL VAWLIEEHLT MSTVAQSRDL 610 620 630 640 650 660 SDRKTIENFA AVVQSVEQMK LLTILTTADI RGVGPGVWNG WKAQLLRSLY YETEPVLTGG 670 680 690 700 710 720 FSEVDRGKRL TAAYAEFRNA FAEWPADELD AYIARHYPAY WLKVELPRKI RHARFVRSSE 730 740 750 760 770 780 QAGHKLAINV GFDEVRGVTE LTIFAADHPW LLSIIAGACA SAGANIVDAQ IYTTTDGRAL 790 800 810 820 830 840 DTISISREYD RDEDEGRRAT RIGEMIEDVL EGKLRLPEVV ARRTVRSKAR PFVIEPEVTI 850 860 870 880 890 900 NNQWSDRYTV IEVSGLDRPG LLYELTTAIS KLNLNIASAH VATFGERARD VFYVTDLLGA 910 920 QINAPTRQSA IKSALTHVMA GDKAVQPAA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC46181.1. Different initiation.
RefSeq	NP_767556.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89VX9.
ModBase	Search...
Protein-protein interaction databases
STRING	224911.bll0916.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC46181; BAC46181; BAC46181.
GeneID	1049859.
KEGG	bja:bll0916.
PATRIC	21185328. VBIBraJap65052_0935.
Phylogenomic databases
eggNOG	COG2844.
HOGENOM	HOG000261779.
KO	K00990.
OMA	SPKVWNA.
ProtClustDB	PRK05092.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-925-MONOMER.
Family and domain databases
Gene3D	1.10.3210.10. 1 hit.
HAMAP	MF_00277. PII_uridylyl-transf.
InterPro	IPR002912. ACT_dom. IPR010043. GlnD_Uridyltrans. IPR003607. HD/PDEase_dom. IPR006674. HD_domain. IPR002934. Nucleotidyltransferase. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
PANTHER	PTHR13734:SF1. PTHR13734:SF1. 1 hit.
Pfam	PF01842. ACT. 2 hits. PF08335. GlnD_UR_UTase. 1 hit. PF01966. HD. 1 hit. PF01909. NTP_transf_2. 1 hit. [Graphical view]
PIRSF	PIRSF006288. PII_uridyltransf. 1 hit.
SMART	SM00471. HDc. 1 hit. [Graphical view]
TIGRFAMs	TIGR01693. UTase_glnD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLND_BRAJA

Accession

Primary (citable) accession number: Q89VX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 5, 2004
Last sequence update:	July 5, 2004
Last modified:	May 1, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program