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Q89VT5

- MTAP_BRADU

UniProt

Q89VT5 - MTAP_BRADU

Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

mtnP

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 65 (01 Oct 2014)
      Sequence version 1 (01 Jun 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

    Catalytic activityi

    S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei12 – 121PhosphateUniRule annotation
    Sitei167 – 1671Important for substrate specificityUniRule annotation
    Binding sitei185 – 1851Substrate; via amide nitrogenUniRule annotation
    Binding sitei186 – 1861PhosphateUniRule annotation
    Sitei222 – 2221Important for substrate specificityUniRule annotation

    GO - Molecular functioni

    1. phosphorylase activity Source: InterPro
    2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
    2. purine ribonucleoside salvage Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine salvage

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-969-MONOMER.
    UniPathwayiUPA00904; UER00873.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
    Alternative name(s):
    5'-methylthioadenosine phosphorylaseUniRule annotation
    Short name:
    MTA phosphorylaseUniRule annotation
    Short name:
    MTAPUniRule annotation
    Gene namesi
    Name:mtnPUniRule annotation
    Ordered Locus Names:blr0960
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 291291S-methyl-5'-thioadenosine phosphorylasePRO_0000415090Add
    BLAST

    Proteomic databases

    PRIDEiQ89VT5.

    Interactioni

    Subunit structurei

    Homohexamer. Dimer of a homotrimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224911.blr0960.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89VT5.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni54 – 552Phosphate bindingUniRule annotation
    Regioni87 – 882Phosphate bindingUniRule annotation
    Regioni209 – 2113Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000228986.
    KOiK00772.
    OrthoDBiEOG6KHFXC.
    PhylomeDBiQ89VT5.

    Family and domain databases

    Gene3Di3.40.50.1580. 1 hit.
    HAMAPiMF_01963. MTAP.
    InterProiIPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view]
    PANTHERiPTHR11904. PTHR11904. 1 hit.
    PfamiPF01048. PNP_UDP_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF53167. SSF53167. 1 hit.
    TIGRFAMsiTIGR01694. MTAP. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q89VT5-1 [UniParc]FASTAAdd to Basket

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    MTQAVLGIIG GSGIYDLPGL EGAHEEVIKS PWGEPSAPLR RGTIAGLPIV    50
    FLPRHDKGHR LSPSDINYRA NIDVLKRAGV TDLISLSACG SFKEEMPPGT 100
    FVLVDQFVDR THKRESSFFG RGCVAHVSMA HPVSPRLRIH LAAAAEAEGI 150
    AIARGGTYVC MEGPQFSTYA ESMTYKTSGY SVIGMTNMPE AKLAREAEIC 200
    YATVAMVTDF DCWHPDHDAV TVQDIIRVLT SNADKAKALV ARLAKDFPRE 250
    HEPCPIGSDR ALDTALITAP EARDPELLKK LDAVAGRVLR G 291
    Length:291
    Mass (Da):31,461
    Last modified:June 1, 2003 - v1
    Checksum:i4C5769311BFC605E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC46225.1.
    RefSeqiNP_767600.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC46225; BAC46225; BAC46225.
    GeneIDi1048928.
    KEGGibja:blr0960.
    PATRICi21185422. VBIBraJap65052_0982.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC46225.1 .
    RefSeqi NP_767600.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali Q89VT5.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.blr0960.

    Proteomic databases

    PRIDEi Q89VT5.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC46225 ; BAC46225 ; BAC46225 .
    GeneIDi 1048928.
    KEGGi bja:blr0960.
    PATRICi 21185422. VBIBraJap65052_0982.

    Phylogenomic databases

    HOGENOMi HOG000228986.
    KOi K00772.
    OrthoDBi EOG6KHFXC.
    PhylomeDBi Q89VT5.

    Enzyme and pathway databases

    UniPathwayi UPA00904 ; UER00873 .
    BioCyci BJAP224911:GJEJ-969-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.1580. 1 hit.
    HAMAPi MF_01963. MTAP.
    InterProi IPR010044. MTAP.
    IPR000845. Nucleoside_phosphorylase_d.
    IPR001369. PNP/MTAP.
    [Graphical view ]
    PANTHERi PTHR11904. PTHR11904. 1 hit.
    Pfami PF01048. PNP_UDP_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53167. SSF53167. 1 hit.
    TIGRFAMsi TIGR01694. MTAP. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiMTAP_BRADU
    AccessioniPrimary (citable) accession number: Q89VT5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 25, 2012
    Last sequence update: June 1, 2003
    Last modified: October 1, 2014
    This is version 65 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3