mtnP

Functionⁱ

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityⁱ

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayⁱ: L-methionine biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:

S-methyl-5'-thioadenosine phosphorylase (mtnP)

This subpathway is part of the pathway L-methionine biosynthesis via salvage pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	12	PhosphateUniRule annotation	1
Siteⁱ	167	Important for substrate specificityUniRule annotation	1
Binding siteⁱ	185	Substrate; via amide nitrogenUniRule annotation	1
Binding siteⁱ	186	PhosphateUniRule annotation	1
Siteⁱ	222	Important for substrate specificityUniRule annotation	1

GO - Molecular functionⁱ

S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-EC

Complete GO annotation...

GO - Biological processⁱ

L-methionine salvage from methylthioadenosine Source: UniProtKB-UniPathway
purine ribonucleoside salvage Source: UniProtKB-KW

Complete GO annotation...

Keywordsⁱ

Molecular function	Glycosyltransferase, Transferase
Biological process	Purine salvage

Enzyme and pathway databases

UniPathwayⁱ	UPA00904; UER00873.

UniPathwayⁱ

UPA00904; UER00873.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation) Alternative name(s): 5'-methylthioadenosine phosphorylaseUniRule annotation Short name: MTA phosphorylaseUniRule annotation Short name: MTAPUniRule annotation
Gene namesⁱ	Name:mtnPUniRule annotation Ordered Locus Names:blr0960
Organismⁱ	Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifierⁱ	224911 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium diazoefficiens
Proteomesⁱ	UP000002526 Componentⁱ: Chromosome

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000415090	1 – 291	S-methyl-5'-thioadenosine phosphorylaseAdd BLAST		291

Proteomic databases

PRIDEⁱ	Q89VT5.

PRIDEⁱ

Q89VT5.

Interactionⁱ

Subunit structureⁱ

Homohexamer. Dimer of a homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGⁱ	224911.blr0960.

STRINGⁱ

224911.blr0960.

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q89VT5.
SMRⁱ	Q89VT5.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	54 – 55	Phosphate bindingUniRule annotation	2
Regionⁱ	87 – 88	Phosphate bindingUniRule annotation	2
Regionⁱ	209 – 211	Substrate bindingUniRule annotation	3

Sequence similaritiesⁱ

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGⁱ	ENOG4107RHB. Bacteria. COG0005. LUCA.
HOGENOMⁱ	HOG000228986.
InParanoidⁱ	Q89VT5.
KEGG Orthology (KO) More...KOⁱ	K00772.
OMAⁱ	NLYRSWG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H0228.
PhylomeDBⁱ	Q89VT5.

Family and domain databases

HAMAPⁱ	MF_01963. MTAP. 1 hit.
InterProⁱ	View protein in InterPro IPR010044. MTAP. IPR000845. Nucleoside_phosphorylase_d.
PANTHERⁱ	PTHR42679. PTHR42679. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01048. PNP_UDP_1. 1 hit.
SUPFAMⁱ	SSF53167. SSF53167. 1 hit.
TIGRFAMsⁱ	TIGR01694. MTAP. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

Q89VT5-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MTQAVLGIIG GSGIYDLPGL EGAHEEVIKS PWGEPSAPLR RGTIAGLPIV 
        60         70         80         90        100
FLPRHDKGHR LSPSDINYRA NIDVLKRAGV TDLISLSACG SFKEEMPPGT 
       110        120        130        140        150
FVLVDQFVDR THKRESSFFG RGCVAHVSMA HPVSPRLRIH LAAAAEAEGI 
       160        170        180        190        200
AIARGGTYVC MEGPQFSTYA ESMTYKTSGY SVIGMTNMPE AKLAREAEIC 
       210        220        230        240        250
YATVAMVTDF DCWHPDHDAV TVQDIIRVLT SNADKAKALV ARLAKDFPRE 
       260        270        280        290 
HEPCPIGSDR ALDTALITAP EARDPELLKK LDAVAGRVLR G

Length:291

Mass (Da):31,461

Last modified:June 1, 2003 - v1

Checksum:ⁱ4C5769311BFC605E

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BA000040 Genomic DNA. Translation: BAC46225.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_767600.1. NC_004463.1. WP_011083781.1. NZ_CP011360.1.

Genome annotation databases

EnsemblBacteriaⁱ	BAC46225; BAC46225; BAC46225.
GeneIDⁱ	1048928.
KEGGⁱ	bja:blr0960.
PATRICⁱ	fig\|224911.44.peg.348.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	BA000040 Genomic DNA. Translation: BAC46225.1.
NCBI Reference Sequences More...RefSeqⁱ	NP_767600.1. NC_004463.1. WP_011083781.1. NZ_CP011360.1.

3D structure databases

ProteinModelPortalⁱ	Q89VT5.
SMRⁱ	Q89VT5.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	224911.blr0960.

STRINGⁱ

224911.blr0960.

Proteomic databases

PRIDEⁱ	Q89VT5.

PRIDEⁱ

Q89VT5.

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblBacteriaⁱ	BAC46225; BAC46225; BAC46225.
GeneIDⁱ	1048928.
KEGGⁱ	bja:blr0960.
PATRICⁱ	fig\|224911.44.peg.348.

Phylogenomic databases

eggNOGⁱ	ENOG4107RHB. Bacteria. COG0005. LUCA.
HOGENOMⁱ	HOG000228986.
InParanoidⁱ	Q89VT5.
KEGG Orthology (KO) More...KOⁱ	K00772.
OMAⁱ	NLYRSWG.
Database of Orthologous Groups More...OrthoDBⁱ	POG091H0228.
PhylomeDBⁱ	Q89VT5.

Enzyme and pathway databases

UniPathwayⁱ	UPA00904; UER00873.

UniPathwayⁱ

UPA00904; UER00873.

Family and domain databases

HAMAPⁱ	MF_01963. MTAP. 1 hit.
InterProⁱ	View protein in InterPro IPR010044. MTAP. IPR000845. Nucleoside_phosphorylase_d.
PANTHERⁱ	PTHR42679. PTHR42679. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF01048. PNP_UDP_1. 1 hit.
SUPFAMⁱ	SSF53167. SSF53167. 1 hit.
TIGRFAMsⁱ	TIGR01694. MTAP. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MTAP_BRADU
Accessionⁱ	Q89VT5Primary (citable) accession number: Q89VT5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	January 25, 2012
	Last sequence update:	June 1, 2003
	Last modified:	June 7, 2017
	This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Prokaryotic Protein Annotation Program

UniProtKB

UniParc

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Supporting data

UniProtKB - Q89VT5 (MTAP_BRADU)

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S-methyl-5'-thioadenosine phosphorylase

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: L-methionine biosynthesis via salvage pathway

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: L-methionine biosynthesis via salvage pathway

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ