UniProtKB - Q89VT5 (MTAP_BRADU)
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Protein
S-methyl-5'-thioadenosine phosphorylase
Gene
mtnP
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Functioni
Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation
Catalytic activityi
S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation
: L-methionine biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- S-methyl-5'-thioadenosine phosphorylase (mtnP)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 12 | PhosphateUniRule annotation | 1 | |
Sitei | 167 | Important for substrate specificityUniRule annotation | 1 | |
Binding sitei | 185 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 186 | PhosphateUniRule annotation | 1 | |
Sitei | 222 | Important for substrate specificityUniRule annotation | 1 |
GO - Molecular functioni
- S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-EC
GO - Biological processi
- L-methionine salvage from methylthioadenosine Source: UniProtKB-UniPathway
- purine ribonucleoside salvage Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine salvage |
Enzyme and pathway databases
BioCyci | BDIA224911:G1G3J-969-MONOMER. |
UniPathwayi | UPA00904; UER00873. |
Names & Taxonomyi
Protein namesi | Recommended name: S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)Alternative name(s): 5'-methylthioadenosine phosphorylaseUniRule annotation Short name: MTA phosphorylaseUniRule annotation Short name: MTAPUniRule annotation |
Gene namesi | Name:mtnPUniRule annotation Ordered Locus Names:blr0960 |
Organismi | Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) |
Taxonomic identifieri | 224911 [NCBI] |
Taxonomic lineagei | Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000415090 | 1 – 291 | S-methyl-5'-thioadenosine phosphorylaseAdd BLAST | 291 |
Proteomic databases
PRIDEi | Q89VT5. |
Interactioni
Subunit structurei
Homohexamer. Dimer of a homotrimer.UniRule annotation
Protein-protein interaction databases
STRINGi | 224911.blr0960. |
Structurei
3D structure databases
ProteinModelPortali | Q89VT5. |
SMRi | Q89VT5. |
ModBasei | Search... |
MobiDBi | Search... |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 54 – 55 | Phosphate bindingUniRule annotation | 2 | |
Regioni | 87 – 88 | Phosphate bindingUniRule annotation | 2 | |
Regioni | 209 – 211 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | ENOG4107RHB. Bacteria. COG0005. LUCA. |
HOGENOMi | HOG000228986. |
InParanoidi | Q89VT5. |
KOi | K00772. |
OMAi | NLYRSWG. |
OrthoDBi | POG091H0228. |
PhylomeDBi | Q89VT5. |
Family and domain databases
HAMAPi | MF_01963. MTAP. 1 hit. |
InterProi | View protein in InterPro IPR010044. MTAP. IPR000845. Nucleoside_phosphorylase_d. IPR035994. Nucleoside_phosphorylase_sf. |
PANTHERi | PTHR42679. PTHR42679. 1 hit. |
Pfami | View protein in Pfam PF01048. PNP_UDP_1. 1 hit. |
SUPFAMi | SSF53167. SSF53167. 1 hit. |
TIGRFAMsi | TIGR01694. MTAP. 1 hit. |
i Sequence
Sequence statusi: Complete.
Q89VT5-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTQAVLGIIG GSGIYDLPGL EGAHEEVIKS PWGEPSAPLR RGTIAGLPIV
60 70 80 90 100
FLPRHDKGHR LSPSDINYRA NIDVLKRAGV TDLISLSACG SFKEEMPPGT
110 120 130 140 150
FVLVDQFVDR THKRESSFFG RGCVAHVSMA HPVSPRLRIH LAAAAEAEGI
160 170 180 190 200
AIARGGTYVC MEGPQFSTYA ESMTYKTSGY SVIGMTNMPE AKLAREAEIC
210 220 230 240 250
YATVAMVTDF DCWHPDHDAV TVQDIIRVLT SNADKAKALV ARLAKDFPRE
260 270 280 290
HEPCPIGSDR ALDTALITAP EARDPELLKK LDAVAGRVLR G
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | BA000040 Genomic DNA. Translation: BAC46225.1. |
RefSeqi | NP_767600.1. NC_004463.1. WP_011083781.1. NZ_CP011360.1. |
Genome annotation databases
EnsemblBacteriai | BAC46225; BAC46225; BAC46225. |
GeneIDi | 1048928. |
KEGGi | bja:blr0960. |
PATRICi | fig|224911.44.peg.348. |
Similar proteinsi
Entry informationi
Entry namei | MTAP_BRADU | |
Accessioni | Q89VT5Primary (citable) accession number: Q89VT5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 25, 2012 |
Last sequence update: | June 1, 2003 | |
Last modified: | February 28, 2018 | |
This is version 81 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |