Q89VE9 (ARGD1_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Acetylornithine aminotransferase 1
Short name=ACOAT 1
EC=2.6.1.11

Gene names

Name:	argD1
Ordered Locus Names:	blr1098

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	405 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + 2-oxoglutarate = N-acetyl-L-glutamate 5-semialdehyde + L-glutamate. HAMAP MF_01107
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP MF_01107
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-L-ornithine from L-glutamate: step 4/4. HAMAP MF_01107
Subunit structure	Homodimer By similarity. HAMAP MF_01107
Subcellular location	Cytoplasm Probable HAMAP MF_01107.
Miscellaneous	May also have succinyldiaminopimelate aminotransferase activity, thus carrying out the corresponding step in lysine biosynthesis. HAMAP MF_01107
Sequence similarities	Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. ArgD subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N2-acetyl-L-ornithine:2-oxoglutarate 5-aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 405	405	Acetylornithine aminotransferase 1 HAMAP MF_01107		PRO_0000112729
Regions
	Region	221 – 224	4	Pyridoxal phosphate binding By similarity
Sites
	Binding site	136	1	Pyridoxal phosphate; via carbonyl oxygen By similarity
	Binding site	139	1	N2-acetyl-L-ornithine By similarity
	Binding site	278	1	N2-acetyl-L-ornithine By similarity
	Binding site	279	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	250	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89VE9 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 3747C89A2F77AECC	FASTA	405	42,747
	10 20 30 40 50 60 MIVMTNSAAP HLLPVFARSD LGFERGEGCW LIATNGDRYL DFTSGVAVNA LGHAHPALVK 70 80 90 100 110 120 ALQEQATKLW HMSNLFQSPD GEKLATRLCN ESFADFVFFC NSGAEALEGV IKLVRHHHFS 130 140 150 160 170 180 KGHPERYRII TFEGAFHGRT LATLAATGSA KYLEGFGPPM DGFDQVPHGD IEAVKKAIGP 190 200 210 220 230 240 QTAGILIEPI QGEGGVRSAT PAFLKALRQL CDEKGLLLAF DEVQTGMGRT GDLFAHRRTG 250 260 270 280 290 300 VTPDVMSLAK ALGGGFPIGA ILATADAAAG MGPGSHGSTF GGNPLAIAAA NAVLDVMLKP 310 320 330 340 350 360 GFFDHVQKMS LLLKQKLASV IDRHGDVVSE VRGEGLLIGI KAVVPSGDLV AALRNEKLLT 370 380 390 400 VGAGDNVVRF LPPLIVTEAE IEDSVGRLER ACAAISSSQT KRAAS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC46363.1.
RefSeq	NP_767738.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89VE9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1049596.
GenomeReviews	Gene locus blr1098 in contig BA000040_GR.
KEGG	bja:blr1098.
NMPDR	fig|224911.1.peg.1098.
PATRIC	21185726. VBIBraJap65052_1134.
Phylogenomic databases
HOGENOM	HBG725944.
OMA	GRKLWHI.
PhylomeDB	Q89VE9.
ProtClustDB	PRK01278.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR1098-MONOMER.
Family and domain databases
HAMAP	MF_01107. ArgD_aminotrans_3. [Tree]
InterPro	IPR004636. AcOrn/SuccinylOrn_aminoTrfase. IPR005814. Aminotrans_3. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 2 hits.
KO	K00821.
PANTHER	PTHR11986. Aminotrans_3. 1 hit. PTHR11986:SF19. ArgD_aminotrans. 1 hit.
Pfam	PF00202. Aminotran_3. 1 hit. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR00707. ArgD. 1 hit.
PROSITE	PS00600. AA_TRANSFER_CLASS_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ARGD1_BRAJA

Accession

Primary (citable) accession number: Q89VE9

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program