Reviewed, UniProtKB/Swiss-Prot Q89VA7 (PYRD_BRAJA)

Names and origin

Protein names

Recommended name:
    Dihydroorotate dehydrogenase
    EC=1.3.5.2
Alternative name(s):
    Dihydroorotate oxidase
    DHOdehase
      Short name=DHODase
      Short name=DHOD

Gene names

Name:	pyrD
Ordered Locus Names:	blr1140

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	365 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-dihydroorotate + a quinone = orotate + a quinol. HAMAP MF_00225
Cofactor	Binds 1 FMN per subunit By similarity.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (quinone route): step 1/1. HAMAP MF_00225
Subunit structure	Homodimer By similarity.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity.
Sequence similarities	Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Cellular component	Cell membrane Membrane
Ligand	FMN Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro UMP biosynthetic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	dihydroorotate oxidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 365	365	Dihydroorotate dehydrogenase HAMAP MF_00225		PRO_1000024155
Sites
	Active site	173	1	Nucleophile By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89VA7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 98FEBC2B6DA8C744	FASTA	365	39,336
	10 20 30 40 50 60 MIRAFDALSL PVLRWLDPED AHRLAIQGLR FLPPVKPRAD DPKLAVRAFG LNFPNPIGMA 70 80 90 100 110 120 AGFDKSAEVP DALLRLGFGF VEIGSVTPKP QSGNPRPRLF RLERDEAVIN RMGFNNDGAD 130 140 150 160 170 180 VALRRLAARA QHGGIVGVNV GANKDSPDRV GDYVKLIEAF APVASYFTIN VSSPNTPGLR 190 200 210 220 230 240 NLQEGALLDD LLARVIDARE RVRQKAGDTP VLLKIAPDLS LAQLDDVVQV ARSRRVDGMI 250 260 270 280 290 300 VSNTTIARPS TLREEMRAKE QGGLSGRPLF RLSTRMVAET YVRVEGAFPL VGVGGVDSGG 310 320 330 340 350 360 AALTKIRAGA SLIQLYSSLV YKGLGLVEEI KRDLTSTLLR TGRDSLSDIV GADAATLTAE DWPGM

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC46405.1.
RefSeq	NP_767780.1.
3D structure databases
HSSP	HSSP built from PDB template 1D3G based on UniProtKB Q02127.
ModBase	Search...
Genome annotation databases
GeneID	1049752.
GenomeReviews	Gene locus blr1140 in contig BA000040_GR.
KEGG	bja:blr1140.
NMPDR	fig|224911.1.peg.1140.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89VA7.
OMA	AALNRMG.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR1140-MON.
BRENDA	1.3.3.1. 280.
Family and domain databases
HAMAP	MF_00225. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR012135. Dihydroorotate_DH_1_2. IPR005719. Dihydroorotate_DH_2. IPR001295. Dihydroorotate_DH_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
Pfam	PF01180. DHO_dh. 1 hit. [Graphical view]
PIRSF	PIRSF000164. DHO_oxidase. 1 hit.
TIGRFAMs	TIGR01036. pyrD_sub2. 1 hit.
PROSITE	PS00911. DHODEHASE_1. 1 hit. PS00912. DHODEHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PYRD_BRAJA

Accession

Primary (citable) accession number: Q89VA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)