Reviewed, UniProtKB/Swiss-Prot Q89UU5 (ISPH1_BRAJA)

Names and origin

Protein names

Recommended name:
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1
    EC=1.17.1.2

Gene names

Name:	ispH1
Ordered Locus Names:	blr1314

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	322 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity.
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity.
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the ispH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 322	322	4-hydroxy-3-methylbut-2-enyl diphosphate reductase 1 HAMAP MF_00191		PRO_0000128784

Sequences

	Sequence		Length	Mass (Da)
	Q89UU5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2E5AE68EDA7D1B3E	FASTA	322	34,976
	10 20 30 40 50 60 MSAKPDLKIV LCSPRGFCAG VVRAIDTVER ALDKYGAPVY VRHEIVHNKY VVDGLKKKGA 70 80 90 100 110 120 IFVEELAEIP ESTTAPVVFS AHGVPKSVPA DAQSRNLFSL DATCPLVTKV HREAAIHFKR 130 140 150 160 170 180 GREIFLIGHS HHPEVVGTLG QLPVGAVTLI ETAEDAKTIT PKDPNNLAFV TQTTLSIDDT 190 200 210 220 230 240 AEIVALLKER FPNINGPHKE DICYATTNRQ LAVKKVAPVV DALIVVGAPN SSNSQRLREV 250 260 270 280 290 300 AEREGCPIAV LAQRAADLDW KRFENITSLG ITAGASAPEV IVEEIMDAFA ERFTLHVETV 310 320 SAAEENEFFP LPRQVRPEAA AE

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC46579.1.
RefSeq	NP_767954.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1049271.
GenomeReviews	Gene locus blr1314 in contig BA000040_GR.
KEGG	bja:blr1314.
NMPDR	fig|224911.1.peg.1314.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89UU5.
OMA	PVIFSAH.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR1314-MON.
BRENDA	1.17.1.2. 280.
Family and domain databases
HAMAP	MF_00191. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. ispH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH1_BRAJA

Accession

Primary (citable) accession number: Q89UU5

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)