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Protein

Glycerol kinase

Gene

glpK

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei11 – 111SubstrateUniRule annotation
Binding sitei15 – 151ATPUniRule annotation
Binding sitei133 – 1331SubstrateUniRule annotation
Binding sitei264 – 2641ATPUniRule annotation
Binding sitei307 – 3071ATP; via carbonyl oxygenUniRule annotation
Binding sitei311 – 3111ATP; via amide nitrogenUniRule annotation
Binding sitei326 – 3261ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 133ATPUniRule annotation
Nucleotide bindingi411 – 4155ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1428-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:blr1410
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
Proteomesi
  • UP000002526 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 500500Glycerol kinasePRO_0000059439Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.blr1410.

Structurei

3D structure databases

ProteinModelPortaliQ89UK6.
SMRiQ89UK6. Positions 2-491.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 822Substrate bindingUniRule annotation
Regioni242 – 2432Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JQ0. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
InParanoidiQ89UK6.
KOiK00864.
OrthoDBiEOG6RZB46.
PhylomeDBiQ89UK6.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89UK6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFVLAIDQG TTSSRAIVFR SDISIAARAQ AEFPQHFPAS GWVEHEPEDI
60 70 80 90 100
WTSTVMVCRD AIAQAGISAK DIAAIGITNQ RETTVVWDRA TGQAVHRAIV
110 120 130 140 150
WQDRRTADIC AKLKADGREP VISEKTGLII DPYFSGTKVA WILDHVPGAR
160 170 180 190 200
ARAARGELMF GTVDCYLLWR LTGGKVHATD ATNASRTLLF NIHTGQWDDE
210 220 230 240 250
LLEIIGVPRS MLPEVKDSSA RFGESVPDLF GGAIAISGIA GDQQAATIGQ
260 270 280 290 300
ACFRPGMMKS TYGTGCFALL NTGTTPVVSK NKLLTTVAYQ LDGKRTYALE
310 320 330 340 350
GSIFVAGSAV QWLRDGLGII KHAAETGPLA DQSDSMQSVY LVPAFVGMGA
360 370 380 390 400
PYWNPRVRGA LFGLTRNTGP AELAHAALES VCYQTFDLWA AMRADWPSSE
410 420 430 440 450
TASVVLRVDG GMTASDWTMQ RLADLLDAPV DRPVIQETTA LGAAYLAGLN
460 470 480 490 500
AGVYPEPTKF ADNWRLEHRF KPNMSEATRE RKLAGWARAV KGVLASDEGA
Length:500
Mass (Da):53,991
Last modified:June 1, 2003 - v1
Checksum:iA540B7FB3153EE3F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46675.1.
RefSeqiNP_768050.1. NC_004463.1.
WP_011084227.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46675; BAC46675; BAC46675.
GeneIDi1055160.
KEGGibja:blr1410.
PATRICi21186408. VBIBraJap65052_1466.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46675.1.
RefSeqiNP_768050.1. NC_004463.1.
WP_011084227.1. NC_004463.1.

3D structure databases

ProteinModelPortaliQ89UK6.
SMRiQ89UK6. Positions 2-491.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.blr1410.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC46675; BAC46675; BAC46675.
GeneIDi1055160.
KEGGibja:blr1410.
PATRICi21186408. VBIBraJap65052_1466.

Phylogenomic databases

eggNOGiENOG4108JQ0. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
InParanoidiQ89UK6.
KOiK00864.
OrthoDBiEOG6RZB46.
PhylomeDBiQ89UK6.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciBJAP224911:GJEJ-1428-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiGLPK_BRADU
AccessioniPrimary (citable) accession number: Q89UK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: November 11, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.