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Q89UK6 (GLPK_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Glycerol kinase
EC=2.7.1.30
Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK
Gene names
Name:glpK
Ordered Locus Names:blr1410
OrganismBradyrhizobium japonicum
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length500 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186

Catalytic activity

ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186

Pathway

Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186

Sequence similarities

Belongs to the FGGY kinase family.

Ontologies

Keywords
   Biological processGlycerol metabolism
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processglycerol-3-phosphate metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

glycerol kinase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 500500Glycerol kinase HAMAP MF_00186
PRO_0000059439

Regions

Nucleotide binding411 – 4155ATP By similarity

Sites

Binding site111Substrate By similarity
Binding site151ATP By similarity
Binding site811Substrate By similarity
Binding site1331Substrate By similarity
Binding site2421Substrate By similarity
Binding site2641ATP By similarity
Binding site3071ATP; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89UK6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: A540B7FB3153EE3F

FASTA50053,991
        10         20         30         40         50         60 
MSFVLAIDQG TTSSRAIVFR SDISIAARAQ AEFPQHFPAS GWVEHEPEDI WTSTVMVCRD 

        70         80         90        100        110        120 
AIAQAGISAK DIAAIGITNQ RETTVVWDRA TGQAVHRAIV WQDRRTADIC AKLKADGREP 

       130        140        150        160        170        180 
VISEKTGLII DPYFSGTKVA WILDHVPGAR ARAARGELMF GTVDCYLLWR LTGGKVHATD 

       190        200        210        220        230        240 
ATNASRTLLF NIHTGQWDDE LLEIIGVPRS MLPEVKDSSA RFGESVPDLF GGAIAISGIA 

       250        260        270        280        290        300 
GDQQAATIGQ ACFRPGMMKS TYGTGCFALL NTGTTPVVSK NKLLTTVAYQ LDGKRTYALE 

       310        320        330        340        350        360 
GSIFVAGSAV QWLRDGLGII KHAAETGPLA DQSDSMQSVY LVPAFVGMGA PYWNPRVRGA 

       370        380        390        400        410        420 
LFGLTRNTGP AELAHAALES VCYQTFDLWA AMRADWPSSE TASVVLRVDG GMTASDWTMQ 

       430        440        450        460        470        480 
RLADLLDAPV DRPVIQETTA LGAAYLAGLN AGVYPEPTKF ADNWRLEHRF KPNMSEATRE 

       490        500 
RKLAGWARAV KGVLASDEGA

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000040 Genomic DNA. Translation: BAC46675.1.
RefSeqNP_768050.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89UK6.
SMRQ89UK6. Positions 2-491.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1055160.
GenomeReviewsGene locus blr1410 in contig BA000040_GR.
KEGGbja:blr1410.
NMPDRfig|224911.1.peg.1410.
PATRIC21186408. VBIBraJap65052_1466.

Phylogenomic databases

HOGENOMHBG511469.
OMACYQTRDL.
PhylomeDBQ89UK6.
ProtClustDBCLSK818559.

Enzyme and pathway databases

BioCycBJAP224911:BLR1410-MONOMER.

Family and domain databases

HAMAPMF_00186. Glycerol_kin.
[Tree]
InterProIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
KOK00864.
PANTHERPTHR10196. FGGY_kin. 1 hit.
PTHR10196:SF9. Glycerol_kin. 1 hit.
PfamPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR01311. Glycerol_kin. 1 hit.
PROSITEPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLPK_BRAJA
AccessionPrimary (citable) accession number: Q89UK6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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