Q89UF8 (UREF_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Urease accessory protein UreF

Gene names

Name:	ureF
Ordered Locus Names:	blr1459

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	242 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required for maturation of urease via the functional incorporation of the urease nickel metallocenter By similarity. HAMAP MF_01385
Subunit structure	UreD, UreF and UreG form a complex that acts as a GTP-hydrolysis-dependent molecular chaperone, activating the urease apoprotein by helping to assemble the nickel containing metallocenter of UreC. The UreE protein probably delivers the nickel By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_01385.
Sequence similarities	Belongs to the UreF family.

Ontologies

Keywords
Biological process	Nickel insertion
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	nitrogen compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	nickel cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 242	242	Urease accessory protein UreF HAMAP MF_01385		PRO_0000344080

Sequences

	Sequence		Length	Mass (Da)
	Q89UF8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DE84BC74342BA435	FASTA	242	25,796
	10 20 30 40 50 60 MLMTTNEPVN AGDLAAREAA ALYRLMTWLS PAFPVGGFSY SSGIEWAVEA GDITDTATLA 70 80 90 100 110 120 DWLDTMLGDG SGFCDATFLV QAYRATEAGE ETSLRDIAEL ASAFVPSRER QLETTSQGRA 130 140 150 160 170 180 FIDIARAAWD AEGLDAMVAA CRTPLVYPVA VGVVAAMHGV PLAPTLHAFL HAVVSNWISA 190 200 210 220 230 240 ASRLIPLGQT DSQRVLVRLE AAVAATATRA LSATLDDLGS ATFRADLASL RHETQYTRLF RS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC46724.1.
RefSeq	NP_768099.1. NC_004463.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1055209.
GenomeReviews	Gene locus blr1459 in contig BA000040_GR.
KEGG	bja:blr1459.
NMPDR	fig|224911.1.peg.1459.
PATRIC	21186514. VBIBraJap65052_1519.
Phylogenomic databases
HOGENOM	HBG709295.
OMA	WLSPAFP.
ProtClustDB	CLSK818588.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR1459-MONOMER.
Family and domain databases
HAMAP	MF_01385. UreF. [Tree]
InterPro	IPR002639. UreF. [Graphical view]
KO	K03188.
Pfam	PF01730. UreF. 1 hit. [Graphical view]
PIRSF	PIRSF009467. Ureas_acces_UreF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

UREF_BRAJA

Accession

Primary (citable) accession number: Q89UF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 22, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program