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Q89UE3

- MASZ_BRADU

UniProt

Q89UE3 - MASZ_BRADU

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Protein

Malate synthase G

Gene
glcB, bll1474
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei274 – 2741Acetyl-CoA By similarity
Binding sitei311 – 3111Acetyl-CoA By similarity
Active sitei339 – 3391Proton acceptor By similarity
Binding sitei339 – 3391Glyoxylate By similarity
Metal bindingi431 – 4311Magnesium By similarity
Binding sitei431 – 4311Glyoxylate By similarity
Metal bindingi459 – 4591Magnesium By similarity
Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei630 – 6301Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1492-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Ordered Locus Names:bll1474
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Malate synthase GUniRule annotationPRO_0000166881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224911.bll1474.

Structurei

3D structure databases

ProteinModelPortaliQ89UE3.
SMRiQ89UE3. Positions 3-719.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA binding By similarity
Regioni456 – 4594Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
KOiK01638.
OrthoDBiEOG6HJ286.
PhylomeDBiQ89UE3.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89UE3-1 [UniParc]FASTAAdd to Basket

« Hide

MKRVDAHGLK IAPVLFDFIA KEAAPKTGIA PDAFWAGVAA IIKDLGPKNR    50
ALLALRDALQ AKIDDWHRAN KGKAFDLNAY TAFLKEIGYL VPEPATQKVE 100
TANVDEEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA FYGTDAIPHD 150
PSESGKGYNK ARGDKVIAKA KAFLDAAVPL ATGSHTDVTA YSIVAGQLAV 200
KLKSGNATAL KNAAQFAGFQ GDAAAPSAVL LVNNGLHVEV TINRNSAIGK 250
DDPAGVADMV MEAAVSTILD MEDSVAAVDA EDKVLVYRNT LGLMNGTLSA 300
DFEKGGKTLT RSLNADRSYK TPDGKGEVKL HGRSLLLMRN CGHHMFTDAV 350
LDERGEEVPE GLLDAAVSGL LAIHDLKGNS KVKNSRTGSA YIVKPKMHGP 400
DEVSFTCEIF DRVEKMLGLP ENTLKVGIMD EERRTTVNLK ACIQRASKRI 450
MFINTGFLDR TGDEIHTSME AGPMIRKNEM KAQAWIKAYE DWNVDMGLID 500
GLPGHAQIGK GMWAAPDKMA DMLAQKLAHP QAGATTAWVP SPTAATLHAL 550
HYHQVNVIAR QEELTKGGPR AKLSDILTIP VSKSNWAPDD VKQEIDNNCQ 600
GILGYVVRWI DQGVGCSKVP DIHDVGLMED RATLRISSQH LANWLHQGVI 650
TEAQVMESLK RMAVVVDKQN AGDAIYKPMA PAFDGVAFKA ACDLIFKGRE 700
QPNGYTEYIL TARRREAKAA G 721
Length:721
Mass (Da):77,928
Last modified:April 30, 2003 - v1
Checksum:iB34A9EBCEDA1422E
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC46739.1.
RefSeqiNP_768114.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46739; BAC46739; BAC46739.
GeneIDi1055224.
KEGGibja:bll1474.
PATRICi21186546. VBIBraJap65052_1535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC46739.1 .
RefSeqi NP_768114.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89UE3.
SMRi Q89UE3. Positions 3-719.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.bll1474.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC46739 ; BAC46739 ; BAC46739 .
GeneIDi 1055224.
KEGGi bja:bll1474.
PATRICi 21186546. VBIBraJap65052_1535.

Phylogenomic databases

eggNOGi COG2225.
HOGENOMi HOG000220740.
KOi K01638.
OrthoDBi EOG6HJ286.
PhylomeDBi Q89UE3.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci BJAP224911:GJEJ-1492-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiMASZ_BRADU
AccessioniPrimary (citable) accession number: Q89UE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: July 9, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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