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Protein

Malate synthase G

Gene

glcB

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei274 – 2741Acetyl-CoAUniRule annotation
Binding sitei311 – 3111Acetyl-CoAUniRule annotation
Active sitei339 – 3391Proton acceptorUniRule annotation
Binding sitei339 – 3391GlyoxylateUniRule annotation
Metal bindingi431 – 4311MagnesiumUniRule annotation
Binding sitei431 – 4311GlyoxylateUniRule annotation
Metal bindingi459 – 4591MagnesiumUniRule annotation
Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei630 – 6301Proton donorUniRule annotation

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1492-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Ordered Locus Names:bll1474
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 721721Malate synthase GPRO_0000166881Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224911.bll1474.

Structurei

3D structure databases

ProteinModelPortaliQ89UE3.
SMRiQ89UE3. Positions 3-719.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
Regioni456 – 4594Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
InParanoidiQ89UE3.
KOiK01638.
OrthoDBiEOG6HJ286.
PhylomeDBiQ89UE3.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89UE3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVDAHGLK IAPVLFDFIA KEAAPKTGIA PDAFWAGVAA IIKDLGPKNR
60 70 80 90 100
ALLALRDALQ AKIDDWHRAN KGKAFDLNAY TAFLKEIGYL VPEPATQKVE
110 120 130 140 150
TANVDEEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA FYGTDAIPHD
160 170 180 190 200
PSESGKGYNK ARGDKVIAKA KAFLDAAVPL ATGSHTDVTA YSIVAGQLAV
210 220 230 240 250
KLKSGNATAL KNAAQFAGFQ GDAAAPSAVL LVNNGLHVEV TINRNSAIGK
260 270 280 290 300
DDPAGVADMV MEAAVSTILD MEDSVAAVDA EDKVLVYRNT LGLMNGTLSA
310 320 330 340 350
DFEKGGKTLT RSLNADRSYK TPDGKGEVKL HGRSLLLMRN CGHHMFTDAV
360 370 380 390 400
LDERGEEVPE GLLDAAVSGL LAIHDLKGNS KVKNSRTGSA YIVKPKMHGP
410 420 430 440 450
DEVSFTCEIF DRVEKMLGLP ENTLKVGIMD EERRTTVNLK ACIQRASKRI
460 470 480 490 500
MFINTGFLDR TGDEIHTSME AGPMIRKNEM KAQAWIKAYE DWNVDMGLID
510 520 530 540 550
GLPGHAQIGK GMWAAPDKMA DMLAQKLAHP QAGATTAWVP SPTAATLHAL
560 570 580 590 600
HYHQVNVIAR QEELTKGGPR AKLSDILTIP VSKSNWAPDD VKQEIDNNCQ
610 620 630 640 650
GILGYVVRWI DQGVGCSKVP DIHDVGLMED RATLRISSQH LANWLHQGVI
660 670 680 690 700
TEAQVMESLK RMAVVVDKQN AGDAIYKPMA PAFDGVAFKA ACDLIFKGRE
710 720
QPNGYTEYIL TARRREAKAA G
Length:721
Mass (Da):77,928
Last modified:April 30, 2003 - v1
Checksum:iB34A9EBCEDA1422E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46739.1.
RefSeqiNP_768114.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46739; BAC46739; BAC46739.
GeneIDi1055224.
KEGGibja:bll1474.
PATRICi21186546. VBIBraJap65052_1535.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46739.1.
RefSeqiNP_768114.1. NC_004463.1.

3D structure databases

ProteinModelPortaliQ89UE3.
SMRiQ89UE3. Positions 3-719.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224911.bll1474.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC46739; BAC46739; BAC46739.
GeneIDi1055224.
KEGGibja:bll1474.
PATRICi21186546. VBIBraJap65052_1535.

Phylogenomic databases

eggNOGiCOG2225.
HOGENOMiHOG000220740.
InParanoidiQ89UE3.
KOiK01638.
OrthoDBiEOG6HJ286.
PhylomeDBiQ89UE3.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciBJAP224911:GJEJ-1492-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiMASZ_BRADU
AccessioniPrimary (citable) accession number: Q89UE3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: April 30, 2003
Last modified: January 7, 2015
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.