Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Q89UE3

- MASZ_BRADU

UniProt

Q89UE3 - MASZ_BRADU

Protein

Malate synthase G

Gene

glcB

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (30 Apr 2003)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei117 – 1171Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei274 – 2741Acetyl-CoAUniRule annotation
    Binding sitei311 – 3111Acetyl-CoAUniRule annotation
    Active sitei339 – 3391Proton acceptorUniRule annotation
    Binding sitei339 – 3391GlyoxylateUniRule annotation
    Metal bindingi431 – 4311MagnesiumUniRule annotation
    Binding sitei431 – 4311GlyoxylateUniRule annotation
    Metal bindingi459 – 4591MagnesiumUniRule annotation
    Binding sitei540 – 5401Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei630 – 6301Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciBJAP224911:GJEJ-1492-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Ordered Locus Names:bll1474
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    ProteomesiUP000002526: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 721721Malate synthase GPRO_0000166881Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei616 – 6161Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi224911.bll1474.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89UE3.
    SMRiQ89UE3. Positions 3-719.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni124 – 1252Acetyl-CoA bindingUniRule annotation
    Regioni456 – 4594Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG2225.
    HOGENOMiHOG000220740.
    KOiK01638.
    OrthoDBiEOG6HJ286.
    PhylomeDBiQ89UE3.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q89UE3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRVDAHGLK IAPVLFDFIA KEAAPKTGIA PDAFWAGVAA IIKDLGPKNR    50
    ALLALRDALQ AKIDDWHRAN KGKAFDLNAY TAFLKEIGYL VPEPATQKVE 100
    TANVDEEIGK ICGPQLVVPL TNARYALNAA NARWGSLYDA FYGTDAIPHD 150
    PSESGKGYNK ARGDKVIAKA KAFLDAAVPL ATGSHTDVTA YSIVAGQLAV 200
    KLKSGNATAL KNAAQFAGFQ GDAAAPSAVL LVNNGLHVEV TINRNSAIGK 250
    DDPAGVADMV MEAAVSTILD MEDSVAAVDA EDKVLVYRNT LGLMNGTLSA 300
    DFEKGGKTLT RSLNADRSYK TPDGKGEVKL HGRSLLLMRN CGHHMFTDAV 350
    LDERGEEVPE GLLDAAVSGL LAIHDLKGNS KVKNSRTGSA YIVKPKMHGP 400
    DEVSFTCEIF DRVEKMLGLP ENTLKVGIMD EERRTTVNLK ACIQRASKRI 450
    MFINTGFLDR TGDEIHTSME AGPMIRKNEM KAQAWIKAYE DWNVDMGLID 500
    GLPGHAQIGK GMWAAPDKMA DMLAQKLAHP QAGATTAWVP SPTAATLHAL 550
    HYHQVNVIAR QEELTKGGPR AKLSDILTIP VSKSNWAPDD VKQEIDNNCQ 600
    GILGYVVRWI DQGVGCSKVP DIHDVGLMED RATLRISSQH LANWLHQGVI 650
    TEAQVMESLK RMAVVVDKQN AGDAIYKPMA PAFDGVAFKA ACDLIFKGRE 700
    QPNGYTEYIL TARRREAKAA G 721
    Length:721
    Mass (Da):77,928
    Last modified:April 30, 2003 - v1
    Checksum:iB34A9EBCEDA1422E
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC46739.1.
    RefSeqiNP_768114.1. NC_004463.1.

    Genome annotation databases

    EnsemblBacteriaiBAC46739; BAC46739; BAC46739.
    GeneIDi1055224.
    KEGGibja:bll1474.
    PATRICi21186546. VBIBraJap65052_1535.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000040 Genomic DNA. Translation: BAC46739.1 .
    RefSeqi NP_768114.1. NC_004463.1.

    3D structure databases

    ProteinModelPortali Q89UE3.
    SMRi Q89UE3. Positions 3-719.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 224911.bll1474.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC46739 ; BAC46739 ; BAC46739 .
    GeneIDi 1055224.
    KEGGi bja:bll1474.
    PATRICi 21186546. VBIBraJap65052_1535.

    Phylogenomic databases

    eggNOGi COG2225.
    HOGENOMi HOG000220740.
    KOi K01638.
    OrthoDBi EOG6HJ286.
    PhylomeDBi Q89UE3.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci BJAP224911:GJEJ-1492-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

    Entry informationi

    Entry nameiMASZ_BRADU
    AccessioniPrimary (citable) accession number: Q89UE3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: April 30, 2003
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3