Q89UE3 (MASZ_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Ordered Locus Names:	bll1474

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium japonicum

Protein attributes

Sequence length	721 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP-Rule MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP-Rule MF_00641
Subunit structure	Monomer By similarity. HAMAP-Rule MF_00641
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glyoxylate cycle Inferred from electronic annotation. Source: HAMAP tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	malate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 721	721	Malate synthase G HAMAP-Rule MF_00641		PRO_0000166881
Sites
	Active site	339	1	Proton acceptor By similarity
	Active site	630	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89UE3 [UniParc]. Last modified April 30, 2003. Version 1. Checksum: B34A9EBCEDA1422E	FASTA	721	77,928
	10 20 30 40 50 60 MKRVDAHGLK IAPVLFDFIA KEAAPKTGIA PDAFWAGVAA IIKDLGPKNR ALLALRDALQ 70 80 90 100 110 120 AKIDDWHRAN KGKAFDLNAY TAFLKEIGYL VPEPATQKVE TANVDEEIGK ICGPQLVVPL 130 140 150 160 170 180 TNARYALNAA NARWGSLYDA FYGTDAIPHD PSESGKGYNK ARGDKVIAKA KAFLDAAVPL 190 200 210 220 230 240 ATGSHTDVTA YSIVAGQLAV KLKSGNATAL KNAAQFAGFQ GDAAAPSAVL LVNNGLHVEV 250 260 270 280 290 300 TINRNSAIGK DDPAGVADMV MEAAVSTILD MEDSVAAVDA EDKVLVYRNT LGLMNGTLSA 310 320 330 340 350 360 DFEKGGKTLT RSLNADRSYK TPDGKGEVKL HGRSLLLMRN CGHHMFTDAV LDERGEEVPE 370 380 390 400 410 420 GLLDAAVSGL LAIHDLKGNS KVKNSRTGSA YIVKPKMHGP DEVSFTCEIF DRVEKMLGLP 430 440 450 460 470 480 ENTLKVGIMD EERRTTVNLK ACIQRASKRI MFINTGFLDR TGDEIHTSME AGPMIRKNEM 490 500 510 520 530 540 KAQAWIKAYE DWNVDMGLID GLPGHAQIGK GMWAAPDKMA DMLAQKLAHP QAGATTAWVP 550 560 570 580 590 600 SPTAATLHAL HYHQVNVIAR QEELTKGGPR AKLSDILTIP VSKSNWAPDD VKQEIDNNCQ 610 620 630 640 650 660 GILGYVVRWI DQGVGCSKVP DIHDVGLMED RATLRISSQH LANWLHQGVI TEAQVMESLK 670 680 690 700 710 720 RMAVVVDKQN AGDAIYKPMA PAFDGVAFKA ACDLIFKGRE QPNGYTEYIL TARRREAKAA G

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC46739.1.
RefSeq	NP_768114.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89UE3.
SMR	Q89UE3. Positions 3-719.
ModBase	Search...
Protein-protein interaction databases
STRING	224911.bll1474.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC46739; BAC46739; BAC46739.
GeneID	1055224.
KEGG	bja:bll1474.
PATRIC	21186546. VBIBraJap65052_1535.
Phylogenomic databases
eggNOG	COG2225.
HOGENOM	HOG000220740.
KO	K01638.
OMA	VVKPKMH.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-1492-MONOMER.
UniPathway	UPA00703; UER00720.
Family and domain databases
Gene3D	2.170.170.11. 2 hits.
HAMAP	MF_00641. Malate_synth_G.
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
PANTHER	PTHR21631:SF1. PTHR21631:SF1. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_BRAJA

Accession

Primary (citable) accession number: Q89UE3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	May 1, 2013
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program