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Q89U95 (PGK_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoglycerate kinase

EC=2.7.2.3
Gene names
Name:pgk
Ordered Locus Names:bll1522
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length398 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate. HAMAP-Rule MF_00145

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5. HAMAP-Rule MF_00145

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00145

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_00145.

Sequence similarities

Belongs to the phosphoglycerate kinase family.

Ontologies

Keywords
   Biological processGlycolysis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglycolytic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

phosphoglycerate kinase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 398398Phosphoglycerate kinase HAMAP-Rule MF_00145
PRO_0000145914

Regions

Nucleotide binding354 – 3574ATP By similarity
Region23 – 253Substrate binding By similarity
Region61 – 644Substrate binding By similarity

Sites

Binding site381Substrate By similarity
Binding site1191Substrate By similarity
Binding site1521Substrate By similarity
Binding site2021ATP By similarity
Binding site3241ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89U95 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 7041F2B5E16AE0E9

FASTA39841,812
        10         20         30         40         50         60 
MANKFRTLDD VDVKGKRVLL RVDLNVPMEN GRVTDATRLE RVAPTITELA DKGGKVILLA 

        70         80         90        100        110        120 
HFGRPKGRDA KESLKPVTEA LSKVLNKPVA FADDCIGEPA ATAVAALKDG DILCLENTRF 

       130        140        150        160        170        180 
HKEEEKNDPA FVAELAKLGD IWVNDAFSAA HRAHASTEGL GHKLPAYAGR TMQAELDALE 

       190        200        210        220        230        240 
KALGSPTKPV IAIIGGAKVS TKIDLLENLV SKVDALVIGG GMANTFLHAQ GVGIGKSLAE 

       250        260        270        280        290        300 
KDLAPTALRI IEKAEAANCA IILPVDATVA YHFAANAPSH AYGLDAIPAD GMILDVGPQS 

       310        320        330        340        350        360 
VARVHAAIDD AATLVWNGPL GAFEMQPFDR GTVAAAKHAA ERTKAKKLIS IAGGGDTVAA 

       370        380        390 
LNQAHVAGDF TYVSTAGGAF LEWMEGKPLP GVEVLRIK 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC46787.1.
RefSeqNP_768162.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89U95.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll1522.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC46787; BAC46787; BAC46787.
GeneID1055272.
KEGGbja:bll1522.
PATRIC21186638. VBIBraJap65052_1581.

Phylogenomic databases

eggNOGCOG0126.
HOGENOMHOG000227107.
KOK00927.
OrthoDBEOG64N9Z0.
PhylomeDBQ89U95.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-1540-MONOMER.
UniPathwayUPA00109; UER00185.

Family and domain databases

Gene3D3.40.50.1260. 1 hit.
3.40.50.1270. 1 hit.
HAMAPMF_00145. Phosphoglyc_kinase.
InterProIPR001576. Phosphoglycerate_kinase.
IPR015901. Phosphoglycerate_kinase_C.
IPR015911. Phosphoglycerate_kinase_CS.
IPR015824. Phosphoglycerate_kinase_N.
[Graphical view]
PANTHERPTHR11406. PTHR11406. 1 hit.
PfamPF00162. PGK. 1 hit.
[Graphical view]
PIRSFPIRSF000724. Pgk. 1 hit.
PRINTSPR00477. PHGLYCKINASE.
SUPFAMSSF53748. SSF53748. 1 hit.
PROSITEPS00111. PGLYCERATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePGK_BRADU
AccessionPrimary (citable) accession number: Q89U95
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways