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Q89U82

- RUVC_BRADU

UniProt

Q89U82 - RUVC_BRADU

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Protein

Crossover junction endodeoxyribonuclease RuvC

Gene

ruvC

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction).UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg(2+) ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161MagnesiumUniRule annotation
Metal bindingi76 – 761MagnesiumUniRule annotation
Metal bindingi148 – 1481MagnesiumUniRule annotation
Metal bindingi151 – 1511MagnesiumUniRule annotation

GO - Molecular functioni

  1. crossover junction endodeoxyribonuclease activity Source: UniProtKB-HAMAP
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. nucleic acid binding Source: InterPro

GO - Biological processi

  1. DNA recombination Source: UniProtKB-HAMAP
  2. DNA repair Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA recombination, DNA repair

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-1553-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Crossover junction endodeoxyribonuclease RuvCUniRule annotation (EC:3.1.22.4UniRule annotation)
Alternative name(s):
Holliday junction nuclease RuvCUniRule annotation
Holliday junction resolvase RuvCUniRule annotation
Gene namesi
Name:ruvCUniRule annotation
Ordered Locus Names:blr1535
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 175175Crossover junction endodeoxyribonuclease RuvCPRO_0000183080Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.blr1535.

Structurei

3D structure databases

ProteinModelPortaliQ89U82.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the RuvC family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0817.
HOGENOMiHOG000012181.
InParanoidiQ89U82.
KOiK01159.
OrthoDBiEOG6RG044.
PhylomeDBiQ89U82.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
HAMAPiMF_00034. RuvC.
InterProiIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamiPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSiPR00696. RSOLVASERUVC.
SUPFAMiSSF53098. SSF53098. 1 hit.
TIGRFAMsiTIGR00228. ruvC. 1 hit.
PROSITEiPS01321. RUVC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89U82-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTALPIRGPV RIIGIDPGLR RTGWGVIEAE GNRLIYVACG SVEPPDDLPL
60 70 80 90 100
SSRLLAIHEG LASVLSDHKP MEAAVEQTFV NKDGVATLKL GQARGVAMLA
110 120 130 140 150
PAMFGISVAE YAPNQVKKTV VGAGHADKQQ IAMMLKILLP KAEPPSADAA
160 170
DALAIAITHA HHRQSTALRL KVVGV
Length:175
Mass (Da):18,417
Last modified:June 1, 2003 - v1
Checksum:i29E9E123C8D935B8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46800.1.
RefSeqiNP_768175.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC46800; BAC46800; BAC46800.
GeneIDi1055285.
KEGGibja:blr1535.
PATRICi21186666. VBIBraJap65052_1595.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC46800.1 .
RefSeqi NP_768175.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89U82.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr1535.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC46800 ; BAC46800 ; BAC46800 .
GeneIDi 1055285.
KEGGi bja:blr1535.
PATRICi 21186666. VBIBraJap65052_1595.

Phylogenomic databases

eggNOGi COG0817.
HOGENOMi HOG000012181.
InParanoidi Q89U82.
KOi K01159.
OrthoDBi EOG6RG044.
PhylomeDBi Q89U82.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-1553-MONOMER.

Family and domain databases

Gene3Di 3.30.420.10. 1 hit.
HAMAPi MF_00034. RuvC.
InterProi IPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view ]
Pfami PF02075. RuvC. 1 hit.
[Graphical view ]
PRINTSi PR00696. RSOLVASERUVC.
SUPFAMi SSF53098. SSF53098. 1 hit.
TIGRFAMsi TIGR00228. ruvC. 1 hit.
PROSITEi PS01321. RUVC. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiRUVC_BRADU
AccessioniPrimary (citable) accession number: Q89U82
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3