Q89TZ6 (PANB1_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferase 1
EC=2.1.2.11

Alternative name(s):
Ketopantoate hydroxymethyltransferase 1
Short name=KPHMT 1

Gene names

Name:	panB1
Ordered Locus Names:	blr1626

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity. HAMAP MF_00156
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00156
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity. HAMAP MF_00156
Subcellular location	Cytoplasm Potential HAMAP MF_00156.
Sequence similarities	Belongs to the PanB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	3-methyl-2-oxobutanoate hydroxymethyltransferase 1 HAMAP MF_00156		PRO_0000297225
Regions
	Region	52 – 53	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	189	1	Proton acceptor By similarity
	Metal binding	52	1	Magnesium By similarity
	Metal binding	91	1	Magnesium By similarity
	Metal binding	122	1	Magnesium By similarity
	Binding site	91	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89TZ6 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 84A5FDD9F8BA1012	FASTA	326	35,372
	10 20 30 40 50 60 MSRVSEQPVD RITIPLLQRW KQDGRRLVMT TAYDAVAAPI PDPSIDISLV GDSGGNVCLG 70 80 90 100 110 120 FENTPPVSVA MMNHHLEAAV RSKPRALLVA DMSFLSFHVS VEETIRNAGG FLQRGAAAAR 130 140 150 160 170 180 LEGGGKRIEM VRAIVDCEIP VMGHLGLVPQ SVNVMGGFKV QGRKVDEALR LLDDAHRLQE 190 200 210 220 230 240 ASCFALVLEG TPAELTARVS DSLTIPTIGI GSGPDCSEQV LCFMTCWAKP KVIAPNSFPP 250 260 270 280 290 300 IRKAFSSSMM RFRAGQRMSA VARSPARKSP IGFPKALGRQ LPTGRHPPQI ITSVAELRRT 310 320 LAKSRSANQR AGLCRRWAIS TMATWR

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC46891.1.
RefSeq	NP_768266.1. NC_004463.1.
3D structure databases
HSSP	HSSP built from PDB template 1OY0 based on UniProtKB P0A5Q8.
ProteinModelPortal	Q89TZ6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1054670.
GenomeReviews	Gene locus blr1626 in contig BA000040_GR.
KEGG	bja:blr1626.
NMPDR	fig|224911.1.peg.1626.
Phylogenomic databases
HOGENOM	HBG299908.
PhylomeDB	Q89TZ6.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR1626-MONOMER.
Family and domain databases
HAMAP	MF_00156. PanB. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
KO	K00606.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
SUPFAM	SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR00222. PanB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB1_BRAJA

Accession

Primary (citable) accession number: Q89TZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 24, 2007
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program