Reviewed, UniProtKB/Swiss-Prot Q89T55 (CHEB2_BRAJA)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	blr2195

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	397 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Miscellaneous	B.japonicum does not have a chemotaxis group 1 operon. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 397	397	Chemotaxis response regulator protein-glutamate methylesterase of group 2 operon HAMAP MF_00099		PRO_0000157982
Regions
	Domain	21 – 139	119	Response regulatory
	Domain	199 – 395	197	CheB-type methylesterase
Sites
	Active site	213	1	By similarity
	Active site	241	1	By similarity
	Active site	337	1	By similarity
Amino acid modifications
	Modified residue	72	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89T55-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: B117DB9518ADD3E7	FASTA	397	41,279
	10 20 30 40 50 60 MSVAFAGNST SGSSREAGPL RVMIVDDSVV IRGLISRWVG AEHDMEVAAS LRTGLEAVNQ 70 80 90 100 110 120 LERINPDVAV LDIEMPELDG ISALPQLLAK KRDLVIIMAS TLTRRNAEIS FKALSLGAAD 130 140 150 160 170 180 YIPKPESTRE ASAADTFHHD LIQKIRHLGA RLRRKAAVAS PPLAPASPPP AARAPFVARP 190 200 210 220 230 240 AAPAPAANAL LPGALSTRPF STLAPKVLLI GSSTGGPQAL MALVTELGPV IDRFPVLITQ 250 260 270 280 290 300 HMPPTFTTIL AEHLARSSRR PAAEAVDGEP VKPGRIYLAP GGKHMRVVRS GADVAIALDD 310 320 330 340 350 360 GPAVNFCKPA VDPLFTSAID IWHGAILSVI LTGMGSDGMR GGKDIVAAGG SVIAQDEASS 370 380 390 VVWGMPGAAA NAGICAAILP LNQIGAKVNR LFAGDRS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC47460.1.
RefSeq	NP_768835.1.
3D structure databases
SMR	Q89T55. Positions 19-396.
ModBase	Search...
Genome annotation databases
GeneID	1055352.
GenomeReviews	Gene locus blr2195 in contig BA000040_GR.
KEGG	bja:blr2195.
NMPDR	fig|224911.1.peg.2195.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG705324.
OMA	IIMASTL.
PhylomeDB	Q89T55.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR2195-MONOMER.
BRENDA	3.1.1.61. 280.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_BRAJA

Accession

Primary (citable) accession number: Q89T55

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2003
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)