Reviewed, UniProtKB/Swiss-Prot Q89T28 (DADA_BRAJA)

Names and origin

Protein names

Recommended name:
    D-amino acid dehydrogenase small subunit
    EC=1.4.99.1

Gene names

Name:	dadA
Ordered Locus Names:	blr2222

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	421 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Oxidative deamination of D-amino acids By similarity. HAMAP MF_01202
Catalytic activity	A D-amino acid + H2O + acceptor = a 2-oxo acid + NH3 + reduced acceptor. HAMAP MF_01202
Cofactor	FAD By similarity. HAMAP MF_01202
Subunit structure	Heterodimer of a small and a large subunit By similarity. HAMAP MF_01202
Sequence similarities	Belongs to the dadA oxidoreductase family.

Ontologies

Keywords
Ligand	FAD Flavoprotein
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine catabolic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW potassium ion transport Inferred from electronic annotation. Source: InterPro
Molecular function	D-amino-acid dehydrogenase activity Inferred from electronic annotation. Source: HAMAP cation transmembrane transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 421	421	D-amino acid dehydrogenase small subunit HAMAP MF_01202		PRO_0000166127
Regions
	Nucleotide binding	3 – 17	15	FAD Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89T28-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 4859B8F71BFCB76C	FASTA	421	45,326
	10 20 30 40 50 60 MKVLILGSGV IGVTSAYYLA GAGHDVTVVD RQPEPALETS FANAGEVSPG YSSPWAGPGV 70 80 90 100 110 120 PVKAVKWLLM KHGPLVIRPK LDPVMWVWLL KMLRNCTSAR YAVNKSRMIP IAEYSRDCLR 130 140 150 160 170 180 DLRRDIGIQY DERSQGTLQL FRHQAQLDGT GEDIAVLKQY GVPFEVLGRE GCIAVEPALA 190 200 210 220 230 240 GVKEKFAGGL RLPQDETGDC HMFTQALAKH AQALGVRFMF NTGIDRIVTD GARVSGVVTS 250 260 270 280 290 300 AGVLQADAYV LALGSWSSRL VAPLGISLPV YPVKGYSITV PIKDASGAPE STVMDESYKV 310 320 330 340 350 360 AITRLGNRIR VGGTAEISGF SSKLYDARRA TLDHSLTDLF PRGGDLSKAT FWSGLRPMTP 370 380 390 400 410 420 DGPPVIGPTQ YANLHLNTGH GTLGWTMSCG SGRVLADMLS GKKPEVDVSA LTVDRYAHRF G

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC47487.1.
RefSeq	NP_768862.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1055326.
GenomeReviews	Gene locus blr2222 in contig BA000040_GR.
KEGG	bja:blr2222.
NMPDR	fig|224911.1.peg.2222.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG729204.
OMA	MFQKHAP.
PhylomeDB	Q89T28.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR2222-MONOMER.
BRENDA	1.4.99.1. 280.
Family and domain databases
HAMAP	MF_01202. DadA. [Tree]
InterPro	IPR006076. FAD-dep_OxRdtase. IPR006036. K_uptake_TrkA. [Graphical view]
Pfam	PF01266. DAO. 1 hit. [Graphical view]
PRINTS	PR00335. KUPTAKETRKA.
ProtoNet	Search...

Entry information

Entry name

DADA_BRAJA

Accession

Primary (citable) accession number: Q89T28

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 27, 2003
Last sequence update:	June 1, 2003
Last modified:	January 19, 2010
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)