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Q89SC2 (HEM6_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 53. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Coproporphyrinogen-III oxidase, aerobic
Short name=Coprogen oxidase
Short name=Coproporphyrinogenase
EC=1.3.3.3
Gene names
Name:hemF
Ordered Locus Names:bll2481
OrganismBradyrhizobium japonicum
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length316 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in heme biosynthesis. Catalyzes the oxidative decarboxylation of propionic acid side chains of rings A and B of coproporphyrinogen III By similarity. HAMAP MF_00333

Catalytic activity

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O. HAMAP MF_00333

Pathway

Porphyrin metabolism; protoporphyrin-IX biosynthesis; protoporphyrinogen-IX from coproporphyrinogen-III (O2 route): step 1/1. HAMAP MF_00333

Subunit structure

Homodimer By similarity. HAMAP MF_00333

Subcellular location

Cytoplasm By similarity HAMAP MF_00333.

Sequence similarities

Belongs to the aerobic coproporphyrinogen-III oxidase family.

Ontologies

Keywords
   Biological processPorphyrin biosynthesis
   Cellular componentCytoplasm
   Molecular functionOxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processporphyrin-containing compound biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncoproporphyrinogen oxidase activity

Inferred from electronic annotation. Source: EC

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 316316Coproporphyrinogen-III oxidase, aerobic HAMAP MF_00333
PRO_0000109886

Regions

Region89 – 9810Important for dimerization By similarity
Region149 – 1513Substrate binding By similarity
Region281 – 31636Important for dimerization By similarity
Region299 – 3046Substrate binding By similarity

Sites

Active site1471Proton donor By similarity
Binding site1331Substrate By similarity
Site2161Important for dimerization By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89SC2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 8698744018E989CF

FASTA31635,498
        10         20         30         40         50         60 
MVGTAQGRLC PPYGRSVREL NMDVSTIEDR KTRARAWFEA LRDDICASFE RLEDDAPQGL 

        70         80         90        100        110        120 
YPGEAGRFTR TPWQRTDHSG AAGGGGVMSM MSGRLFEKVG VHCSTVHGEF APEFRAQIPG 

       130        140        150        160        170        180 
AADDPRFWAS GISLIAHLRN PHVPAVHMNT RFVVTTKAWF GGGADLTPVL DRRRTQDDAD 

       190        200        210        220        230        240 
SIAFHAAMKE ACVQPNGVAD YDKYKKWCDE YFYLPHRKEA RGIGGIFYDW HDSGDWDADL 

       250        260        270        280        290        300 
AFTQDVGRAF LKIYPDIVRR NFASAWTAAD REEQLIRRGR YVEFNLLYDR GTIFGLKTGG 

       310 
NVDSILSSLP PEVKWP

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000040 Genomic DNA. Translation: BAC47746.1.
RefSeqNP_769121.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89SC2.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1050400.
GenomeReviewsGene locus bll2481 in contig BA000040_GR.
KEGGbja:bll2481.
NMPDRfig|224911.1.peg.2481.
PATRIC21188348. VBIBraJap65052_2434.

Phylogenomic databases

HOGENOMHBG631180.
OMAVKAYLLD.
PhylomeDBQ89SC2.
ProtClustDBPRK05330.

Enzyme and pathway databases

BioCycBJAP224911:BLL2481-MONOMER.

Family and domain databases

HAMAPMF_00333. Coprogen_oxidas.
[Tree]
InterProIPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
[Graphical view]
Gene3DG3DSA:3.40.1500.10. Coprogen_oxidas. 1 hit.
KOK00228.
PANTHERPTHR10755. Coprogen_oxidas. 1 hit.
PfamPF01218. Coprogen_oxidas. 1 hit.
[Graphical view]
PIRSFPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSPR00073. COPRGNOXDASE.
SUPFAMSSF102886. Coprogen_oxidas. 1 hit.
PROSITEPS01021. COPROGEN_OXIDASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameHEM6_BRAJA
AccessionPrimary (citable) accession number: Q89SC2
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 53 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

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