Q89S70 (SYGA_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine--tRNA ligase alpha subunit
EC=6.1.1.14

Alternative name(s):
Glycyl-tRNA synthetase alpha subunit
Short name=GlyRS

Gene names

Name:	glyQ
Ordered Locus Names:	blr2535

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-tRNA(Gly). HAMAP MF_00254
Subunit structure	Tetramer of two alpha and two beta subunits By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00254.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	glycyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycine-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	Glycine--tRNA ligase alpha subunit HAMAP MF_00254		PRO_1000078521

Sequences

	Sequence		Length	Mass (Da)
	Q89S70 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 5F11347BAF5FA3FC	FASTA	311	35,262
	10 20 30 40 50 60 MDASLPAHMR PERSFQGFIL ALQRFWAEQG CVILQPYDME MGAGTFHPAT TLRALGPKPW 70 80 90 100 110 120 NAAYVQPSRR PKDGRYGENP NRMQHYYQFQ VIMKPSPPNL QELYLKSLAA IGIDSAVHDI 130 140 150 160 170 180 RFVEDDWESP TLGAWGLGWE CWCDGMEVSQ FTYFQQVAGF ECAPVAGELT YGLERLAMYV 190 200 210 220 230 240 QGVDRVYDLN FNGRDGDAKV TYGDVFLQAE REYSKHNFEV ADTAMLFEQF KMAEAACRKY 250 260 270 280 290 300 LDAGWREGNR KEHLMALPAY DQCIKASHVF NLLDARGVIS VTERQSYILR VRELAKACGE 310 AWIHTEAGGA A

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC47800.1.
RefSeq	NP_769175.1. NC_004463.1.
3D structure databases
HSSP	HSSP built from PDB template 1J5W based on UniProtKB Q9WY59.
ProteinModelPortal	Q89S70.
SMR	Q89S70. Positions 14-307.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1050258.
GenomeReviews	Gene locus blr2535 in contig BA000040_GR.
KEGG	bja:blr2535.
NMPDR	fig|224911.1.peg.2535.
PATRIC	21188468. VBIBraJap65052_2494.
Phylogenomic databases
HOGENOM	HBG285316.
OMA	CRRPTDG.
PhylomeDB	Q89S70.
ProtClustDB	PRK09348.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR2535-MONOMER.
Family and domain databases
HAMAP	MF_00254. Gly_tRNA_synth_alpha. [Tree]
InterPro	IPR006194. Gly-tRNA-synth_II_heterodimer. IPR002310. Gly-tRNA_synth_IIc_asu. [Graphical view]
KO	K01878.
Pfam	PF02091. tRNA-synt_2e. 1 hit. [Graphical view]
PRINTS	PR01044. TRNASYNTHGA.
TIGRFAMs	TIGR00388. GlyQ. 1 hit.
PROSITE	PS50861. AA_TRNA_LIGASE_II_GLYAB. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYGA_BRAJA

Accession

Primary (citable) accession number: Q89S70

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program