Reviewed, UniProtKB/Swiss-Prot Q89RW1 (DXS_BRAJA)

Names and origin

Protein names

Recommended name:
    1-deoxy-D-xylulose-5-phosphate synthase
    EC=2.2.1.7
Alternative name(s):
    1-deoxyxylulose-5-phosphate synthase
      Short name=DXP synthase
      Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	bll2651

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	661 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity.
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP thiamin biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 661	661	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189091

Sequences

	Sequence		Length	Mass (Da)
	Q89RW1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 277A10C465A60BD5	FASTA	661	71,263
	10 20 30 40 50 60 MQTHRAPERP SGADKLEIAV NAYSKTPLLD TIRTPDDLRK LKIEQVRQVA DELRQETIDA 70 80 90 100 110 120 VSVTGGHFGA GLGVVELTTA IHYVFDTPRD RLIWDVGHQA YPHKILTGRR DRIRTLRTGG 130 140 150 160 170 180 GLSGFTKRSE SDYDPFGAAH SSTSISAGLG MAVARDLSGG KNNVIAVIGD GAMSAGMAYE 190 200 210 220 230 240 AMNNAGAMNS RLIVILNDND MSIAPPVGAM SAYLSRLYSG KTYRTLREAA KQINKRLPKI 250 260 270 280 290 300 IANRANRVEE YSRGFMMDGG TLFEELGFYY VGPIDGHNLD HLLPVLKNVR DMEEGPILVH 310 320 330 340 350 360 VVTQKGKGYG PAEASADKYH AVVKFDVATG TQAKAKPNAP AYQNVFGQSL VKEAQKDEKI 370 380 390 400 410 420 VAITAAMPSG TGVDIFNKAF PDRTFDVGIA EQHAVTFAAG LASEGYKPFC AIYSTFLQRG 430 440 450 460 470 480 YDQIVHDVAI QNLPVRFAID RAGLVGADGA THAGSFDNAY LGCLPNMVIM AAADEAELVH 490 500 510 520 530 540 MVATQVAIDD RPSSLRYPRG EGRGIEMPEV GIPLEIGKGR MIRQGSKIAL LSFGTRLAEC 550 560 570 580 590 600 EKAADELAAH GLSTTIADAR FMKPLDTELV LKLARDHEIL ITIEEGSVGG FGSHVAQFLT 610 620 630 640 650 660 DQGALDSGMV KFRTMVLPDV FQDHDTPAAM YARAGLDAKG IVAKVFEALG KDVKTETVKL A

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC47916.1.
RefSeq	NP_769291.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1048765.
GenomeReviews	Gene locus bll2651 in contig BA000040_GR.
KEGG	bja:bll2651.
NMPDR	fig|224911.1.peg.2651.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89RW1.
OMA	QRFPDRY.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL2651-MON.
BRENDA	2.2.1.7. 280.
Family and domain databases
HAMAP	MF_00315. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
TIGRFAMs	TIGR00204. dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_BRAJA

Accession

Primary (citable) accession number: Q89RW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)