Q89QW7 (ISPH2_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2
EC=1.17.1.2

Gene names

Name:	ispH2
Ordered Locus Names:	bll3007

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	4-hydroxy-3-methylbut-2-enyl diphosphate reductase 2 HAMAP MF_00191		PRO_0000128785

Sequences

	Sequence		Length	Mass (Da)
	Q89QW7 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E3CEE308EF73C2A6	FASTA	308	33,269
	10 20 30 40 50 60 MEVYLAQPRG FCAGVVRAIE IVERALEKFG PPVYVRHEIV HNKYVVESLK NKGAIFVEEL 70 80 90 100 110 120 SEVPPKAVTV FSAHGVARSV EEEAAARDLP VLNATCPLVT KVHNQGKRYI TKGRTLILIG 130 140 150 160 170 180 HAGHPEVEGT MGQVPAPVLL VQSVEEVNAL TLPADTPVAY ITQTTLSVDD TRDIISALQA 190 200 210 220 230 240 RFTDIQGPDI RDICYATQNR QSAVRDLSKL VDVILVVGAA NSSNSNRLRE IGTEAGVASY 250 260 270 280 290 300 LIADGSELNP EWLKDARTVG VTAGASAPEV LVDDVIEAMR RIGPVKVQVL PGREENIEFR LPAELAAS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC48272.1.
RefSeq	NP_769647.1. NC_004463.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1053375.
GenomeReviews	Gene locus bll3007 in contig BA000040_GR.
KEGG	bja:bll3007.
NMPDR	fig|224911.1.peg.3007.
PATRIC	21189464. VBIBraJap65052_2990.
Phylogenomic databases
HOGENOM	HBG335228.
OMA	HNQGKRY.
PhylomeDB	Q89QW7.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL3007-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH2_BRAJA

Accession

Primary (citable) accession number: Q89QW7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2004
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program