Q89QU8 (DNAE2_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	blr3026

Organism

Bradyrhizobium japonicum

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	1151 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1151	1151	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103369

Sequences

	Sequence		Length	Mass (Da)
	Q89QU8 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 2F0563C7FC4625A7	FASTA	1,151	129,582
	10 20 30 40 50 60 MNTPAYAEIG ITTNFSFLRG GSDPRAYVHQ ASILGISAIG IADHNTLAGV VRAYKELDND 70 80 90 100 110 120 KVLHKPKLLI GARIVFIDGT PDILVYPRDR AAYGRLCQLL TRGKRGDDVT RIEKGECRLT 130 140 150 160 170 180 FSDLLAFSEG QLLVLTLPHR FEPAQALDVL AKLKATRAEG VWLAASLVHR GDDRRRLARL 190 200 210 220 230 240 DDLATTAKVQ LLATNEVLYH DPARRPLQDV LTCIREKTTI EAVGRKLEAN AERFLKTPRE 250 260 270 280 290 300 MSRLFRDFPD AIAETMRFAN KIDFSLDQLR YQYPDEPVPP GKTAQGHLED LTWAGVDKYF 310 320 330 340 350 360 AGKIDDKLRA TLKKELALIA ELKYAHYFLT VHDIVHYARS QNILCQGRGS AANSAVCYVL 370 380 390 400 410 420 GITSVDPTKV DLLFERFISK ERLEPPDIDV DFEHSRREEV MQYVYRRYGR HRAAIIATII 430 440 450 460 470 480 HYRPRSAIRD VGKALGLTED VTAALADTVW GSWGSGLNDM QVKQAGLDPQ NPMINLAVEL 490 500 510 520 530 540 ATELIEFPRH LSQHVGGYVL TQDRLDTYVP IGNAAMDDRT FIEWDKDDVD ALNMMKVDVL 550 560 570 580 590 600 ALGMLTCIRK CFDLIDQHKG ERWVLASVPQ DDPKVYDMLC DGESLGVFQV ESRAQMNMLP 610 620 630 640 650 660 RLKPRTFYDL VIEVAIVRPG PIQGDMVHPY LRRRNGQEKV NYPSPSPEHG PADELYKVLH 670 680 690 700 710 720 KTKGVPLFQE QAMRIAIEAA KFTSEEANGL RRSMATFRNV GTIGKYEDKL IGNMVARGYD 730 740 750 760 770 780 PNFARSCFDQ IKGFGSYGFP ESHAASFAQL VYISSWLKYH HPDAFCCGLL NSQPMGFYAP 790 800 810 820 830 840 AQIVGDARKN GVEVRDIDVS YSFAQNTLEQ GSGKYCAVRL GFRQIDGFHW LDEDEEHLKR 850 860 870 880 890 900 SLLSFRGAPL GANPESIGPH MPGGMDSGLD AGASPRNDKK EDWANRIVAA RNRRPFTSLE 910 920 930 940 950 960 DFARDTGLPK RALILLADAD AFRSLGLDRR EALWQVRRLP DDVPLPLFEA ATAREQPDEH 970 980 990 1000 1010 1020 AKPLPLMPRP EQVVADYQTI RLSLKGHPME FLREMLSRER VVACKDVNHQ NERRRVRCAG 1030 1040 1050 1060 1070 1080 VVLVRQRPGS ASGVVFMTLE DETGIANVVV WPKIMEQYRK EVMGARLILV EGYIQSSPEK 1090 1100 1110 1120 1130 1140 VTHLIAQRMV DRSHDLIGLA NDSLTRKHPV PSGDALIEPL NDDRRDHADA PAQKIRHPRN 1150 VRILPPSRDF H

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC48291.1.
RefSeq	NP_769666.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89QU8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1053315.
GenomeReviews	Gene locus blr3026 in contig BA000040_GR.
KEGG	bja:blr3026.
NMPDR	fig|224911.1.peg.3026.
PATRIC	21189506. VBIBraJap65052_3011.
Phylogenomic databases
HOGENOM	HBG734490.
OMA	EQPAIRL.
PhylomeDB	Q89QU8.
ProtClustDB	PRK05672.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR3026-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR004805. PolC_alpha. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00594. Polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_BRAJA

Accession

Primary (citable) accession number: Q89QU8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	June 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program