Reviewed, UniProtKB/Swiss-Prot Q89QJ5 (GLNE_BRAJA)

Names and origin

Protein names

Recommended name:
    Glutamate-ammonia-ligase adenylyltransferase
    EC=2.7.7.42
Alternative name(s):
    [Glutamate--ammonia-ligase] adenylyltransferase
    Glutamine-synthetase adenylyltransferase
      Short name=ATase

Gene names

Name:	glnE
Ordered Locus Names:	blr3133

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	995 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Adenylation and deadenylation of glutamine synthetase By similarity.
Catalytic activity	ATP + [L-glutamate:ammonia ligase (ADP-forming)] = diphosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]. HAMAP MF_00802
Sequence similarities	Belongs to the glnE family.

Ontologies

Keywords
Domain	Repeat
Ligand	ATP-binding Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW [glutamate-ammonia-ligase] adenylyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 995	995	Glutamate-ammonia-ligase adenylyltransferase HAMAP MF_00802		PRO_0000209234
Regions
	Region	122 – 333	212	GlnE 1 HAMAP MF_00802
	Region	637 – 853	217	GlnE 2 HAMAP MF_00802

Sequences

	Sequence		Length	Mass (Da)
	Q89QJ5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DCF10D729E67A533	FASTA	995	110,119
	10 20 30 40 50 60 MNHSAPGNAD KHGESLAARF AEAPHIAAST TDERRFESWL AELEPAQSAR LEALLVQPFG 70 80 90 100 110 120 RNILVGIAEF SPYLFDLVRA DPLRLIRLLE CDPDTHLAAL IAEARGAVLA APDEAEVMRL 130 140 150 160 170 180 LRRMKAEAAL LTALCDIGGV WPVMRVTSAL TDVAVSSVQA ALQYLLRQEA ARGKLFPPNP 190 200 210 220 230 240 EAPEEGCGLI VLAMGKMGAG ELNYSSDIDL IVFFDPDATT LAPDIEPQPF FVRVTQGMAR 250 260 270 280 290 300 ILQQRTYDGY VFRVDLRLRP DPSSTQVAIS RDAALHYYER EGRTWERAAM IKARACAGDA 310 320 330 340 350 360 RAGEALLAEI APFVWRKHLD FAALADVHDM KRQMQTYRGQ SEVAVEGHNV KVGRGGIREI 370 380 390 400 410 420 EFFAQTQQLI AGGRHPELRV RPTLAALDVL ASSNWITLAA RDELARAYEF LRRVEHRLQM 430 440 450 460 470 480 VADEQTHALP DDREAVERFA RFFGYPDREA FARDLLRQLE IVQGHYEKLF EGDDPTGTAK 490 500 510 520 530 540 LPALDYSAGP DDPRLFQHLT TLGFKKPAAV AQTVRDWITG DYRVFRNEAT RSAFIEFVPA 550 560 570 580 590 600 LIDGLALAEE PDRAVVAFDQ FLGALQRGGR LITLLGQNRD LVALVALVLG AAPRLGEMLA 610 620 630 640 650 660 RQPQLMDGLI DPRFFGAMPD RRELSGRLAA TVQDAASYEE FLDRLRLFGQ ESLFLIGTRI 670 680 690 700 710 720 LSGTVSAQQA STAFADVAEG VVHTVHDLVA DRFAAQHGRI KGQETAIIAM GRLGSREMTA 730 740 750 760 770 780 SSDLDLILLY DFDSENPDSD GPKSLQGAHY FARFTQRLIS AFTTRTNYGV LYEIDMRLRP 790 800 810 820 830 840 SGRAGPVASS LASFADYQAN EAWTWEHMAL TRARVVSASA EFGERIAGVI RDVLTRRRDP 850 860 870 880 890 900 ATIANDVADM RRAIAQEKGE TDYWDLKYAA GGMIDIDFIA QYLQLVHAHH KPDILDVSTL 910 920 930 940 950 960 QVLDNAARLG VLPQSEAEIL RAAARLYHDL TQILRLCVSD RFKPETAGTD LQRVMARAGD 970 980 990 APDFSSLEAR VKETQSEVRR VFRALLEGTS PASAR

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC48398.1.
RefSeq	NP_769773.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1053028.
GenomeReviews	Gene locus blr3133 in contig BA000040_GR.
KEGG	bja:blr3133.
NMPDR	fig|224911.1.peg.3133.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89QJ5.
OMA	Q89QJ5. WERYAMI.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR3133-MON.
BRENDA	2.7.7.42. 280.
Family and domain databases
HAMAP	MF_00802. [Tree]
InterPro	IPR005190. GlnE. IPR013546. PII_UdlTrfase/GS_AdlTrfase. [Graphical view]
Pfam	PF08335. GlnD_UR_UTase. 1 hit. PF03710. GlnE. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLNE_BRAJA

Accession

Primary (citable) accession number: Q89QJ5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 4, 2005
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)