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Protein

Nitrilase blr3397

Gene

blr3397

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Nitrilase that acts on various kinds of nitrile compounds such as aliphatic and aromatic nitriles. Has higher activity toward aliphatic nitriles compared to aromatic nitriles. Among the different substrates tested, has the highest activity toward hydrocinnamonitrile.1 Publication

Catalytic activityi

R-CN + 2 H2O = R-COOH + NH3.1 Publication

Kineticsi

kcat is 111 min(-1) with phenylacetonitrile.
  1. KM=4.36 mM for phenylacetonitrile1 Publication

    pH dependencei

    Optimum pH is 7.0-8-0.1 Publication

    Temperature dependencei

    Optimum temperature is 45 degrees Celsius.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei50Proton acceptorPROSITE-ProRule annotation1
    Active sitei137Proton donorPROSITE-ProRule annotation1
    Active sitei171NucleophilePROSITE-ProRule annotation1

    GO - Molecular functioni

    • aliphatic nitrilase activity Source: UniProtKB

    GO - Biological processi

    • metabolic process Source: UniProtKB
    • nitrogen compound metabolic process Source: InterPro
    • protein homooligomerization Source: UniProtKB

    Keywordsi

    Molecular functionHydrolase

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nitrilase blr3397 (EC:3.5.5.7)
    Gene namesi
    Ordered Locus Names:blr3397
    OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
    Taxonomic identifieri224911 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
    Proteomesi
    • UP000002526 Componenti: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00004222171 – 321Nitrilase blr3397Add BLAST321

    Interactioni

    Subunit structurei

    Homodecamer.1 Publication

    Protein-protein interaction databases

    STRINGi224911.blr3397.

    Structurei

    3D structure databases

    ProteinModelPortaliQ89PT3.
    SMRiQ89PT3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Domaini10 – 277CN hydrolasePROSITE-ProRule annotationAdd BLAST268

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiENOG4105ES4. Bacteria.
    COG0388. LUCA.
    HOGENOMiHOG000256365.
    InParanoidiQ89PT3.
    KOiK01501.
    OMAiTMRHIAL.
    OrthoDBiPOG091H0LO0.
    PhylomeDBiQ89PT3.

    Family and domain databases

    Gene3Di3.60.110.10. 1 hit.
    InterProiView protein in InterPro
    IPR003010. C-N_Hydrolase.
    IPR036526. C-N_Hydrolase_sf.
    IPR000132. Nitrilase/CN_hydratase_CS.
    PfamiView protein in Pfam
    PF00795. CN_hydrolase. 1 hit.
    SUPFAMiSSF56317. SSF56317. 1 hit.
    PROSITEiView protein in PROSITE
    PS50263. CN_HYDROLASE. 1 hit.
    PS00920. NITRIL_CHT_1. 1 hit.
    PS00921. NITRIL_CHT_2. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q89PT3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MMDSNRPNTY KAAVVQAASD PTSSLVSAQK AAALIEKAAG AGARLVVFPE
    60 70 80 90 100
    AFIGGYPKGN SFGAPVGMRK PEGREAFRLY WEAAIDLDGV EVETIAAAAA
    110 120 130 140 150
    ATGAFTVIGC IEREQGTLYC TALFFDGARG LVGKHRKLMP TAGERLIWGF
    160 170 180 190 200
    GDGSTMPVFE TSLGNIGAVI CWENYMPMLR MHMYSQGISI YCAPTADDRD
    210 220 230 240 250
    TWLPTMQHIA LEGRCFVLTA CQHLKRGAFP ADYECALGAD PETVLMRGGS
    260 270 280 290 300
    AIVNPLGKVL AGPCFEGETI LYADIALDEV TRGKFDFDAA GHYSRPDVFQ
    310 320
    LVVDDRPKRA VSTVSAVRAR N
    Length:321
    Mass (Da):34,475
    Last modified:June 1, 2003 - v1
    Checksum:i561F3156D4BF8B86
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    BA000040 Genomic DNA. Translation: BAC48662.1.
    RefSeqiNP_770037.1. NC_004463.1.
    WP_011086181.1. NZ_CP011360.1.

    Genome annotation databases

    EnsemblBacteriaiBAC48662; BAC48662; BAC48662.
    GeneIDi1050691.
    KEGGibja:blr3397.
    PATRICifig|224911.44.peg.3047.

    Similar proteinsi

    Entry informationi

    Entry nameiNITR2_BRADU
    AccessioniPrimary (citable) accession number: Q89PT3
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: June 1, 2003
    Last modified: October 25, 2017
    This is version 76 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families