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Q89NW6

- LIPA2_BRADU

UniProt

Q89NW6 - LIPA2_BRADU

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Protein

Lipoyl synthase 2

Gene

lipA2

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi67 – 671Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi72 – 721Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi78 – 781Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi93 – 931Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi97 – 971Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi100 – 1001Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-3741-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 2UniRule annotation (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lip-syn 2UniRule annotation
Lipoate synthase 2UniRule annotation
Lipoic acid synthase 2UniRule annotation
Sulfur insertion protein lipA2
Gene namesi
Name:lipA2UniRule annotation
Synonyms:lipA
Ordered Locus Names:bll3717
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 322322Lipoyl synthase 2PRO_0000102294Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi224911.bll3717.

Structurei

3D structure databases

ProteinModelPortaliQ89NW6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235997.
InParanoidiQ89NW6.
KOiK03644.
OrthoDBiEOG6038ZS.
PhylomeDBiQ89NW6.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q89NW6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKVILDLLNN DPRTTQRTER PRHPEKANRP DTPMESRPAW IRVKAPGSAQ
60 70 80 90 100
WTETQRIVRE NKLVTVCEEA SCPNIGECWA KKHATFMIMG DTCTRACAFC
110 120 130 140 150
NVRTGLPGPL DADEPGKVAD AVAKLGLEHV VVTSVDRDDL ADGGAAHFAA
160 170 180 190 200
TIAAIRAMSP ATSIEILTPD FLRKHGALET VVAARPDVLN HNLETVPSKY
210 220 230 240 250
LAVRPGARYF HSVRLLQRAK ELDPRIFTKS GIMVGLGEDR SEVLQLMDDL
260 270 280 290 300
RSADVDFLTI GQYLQPTRKH HAVMRFVPPD EFEAYEKTAY AKGFLMVSAT
310 320
PLTRSSHHAG DDFRKLRERR RA
Length:322
Mass (Da):35,885
Last modified:June 1, 2003 - v1
Checksum:iDC515282CE94A4E7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC48982.1.
RefSeqiNP_770357.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC48982; BAC48982; BAC48982.
GeneIDi1052093.
KEGGibja:bll3717.
PATRICi21190889. VBIBraJap65052_3702.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC48982.1 .
RefSeqi NP_770357.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89NW6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.bll3717.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC48982 ; BAC48982 ; BAC48982 .
GeneIDi 1052093.
KEGGi bja:bll3717.
PATRICi 21190889. VBIBraJap65052_3702.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235997.
InParanoidi Q89NW6.
KOi K03644.
OrthoDBi EOG6038ZS.
PhylomeDBi Q89NW6.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci BJAP224911:GJEJ-3741-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiLIPA2_BRADU
AccessioniPrimary (citable) accession number: Q89NW6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 10, 2004
Last sequence update: June 1, 2003
Last modified: November 26, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3