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Q89NA7 (GLSA1_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaminase 1

EC=3.5.1.2
Gene names
Name:glsA1
Ordered Locus Names:bll3935
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length613 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00313

Sequence similarities

Belongs to the glutaminase family.

Contains 1 cyclic nucleotide-binding domain.

Contains 1 STAS domain.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutaminase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 613613Glutaminase 1 HAMAP-Rule MF_00313
PRO_0000110597

Regions

Domain345 – 457113STAS
Nucleotide binding480 – 595116cNMP HAMAP-Rule MF_00313
Region33 – 315283Glutaminase HAMAP-Rule MF_00313

Sites

Binding site751Substrate By similarity
Binding site1241Substrate By similarity
Binding site1681Substrate By similarity
Binding site1751Substrate By similarity
Binding site1991Substrate By similarity
Binding site2511Substrate By similarity
Binding site2691Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89NA7 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 2F7F6A35D48DA118

FASTA61366,635
        10         20         30         40         50         60 
MTPPPASAWT RSKPPLLRFL DTCLNEFSAE TSGAVADYIP ELGNADPAYF GISLATLDGH 

        70         80         90        100        110        120 
VYEVGDSRVP FTIQSMSKPF VFALALDLLG AGRVESAIGV EPSGDPFNSI RLNSDNHPFN 

       130        140        150        160        170        180 
PMVNAGAIAC TGLIYDSKGA EAFEQIRLAL SRFAGRDLAV DEAVYSSESQ TGDRNRAIGY 

       190        200        210        220        230        240 
LLKTNAVISD NVAAVLDVYF RQCAVLVTAR DIAVMAATLA NRGINPVTGE QVMSAYAISR 

       250        260        270        280        290        300 
TLSVMTSSGM YDYAGEWIYR IGIPAKSGVG GGILAALPAR LGLGSYSPKL DKHGNSVRGI 

       310        320        330        340        350        360 
KVCEALSSHY DLHMLNRSDD ARNAVIADYD IGKSPSRRVR RAQEREILAA HEQEVRIIEL 

       370        380        390        400        410        420 
VGTLSLSAVD YVSRRLAGRP RPQFVIFDLH RVTSTTRAGA RLVAEAFEEL AALNVTVVLS 

       430        440        450        460        470        480 
GVRRASKEWN TLREWTAELK NVRDFYLLDT AIEWAEDQIV YRYGGSIDFH ETTELAEQPL 

       490        500        510        520        530        540 
LEGLSADELA ELGAICTIRT YQSGAKILTT GDPADALFFL RSGAVHVTLP DGVRLATLTA 

       550        560        570        580        590        600 
GMAFGEMALL EQTRSADVFA DMAATAFEAP LKDFERFREQ HPRASERIMR NLAQLLADRL 

       610 
IVANAKVDIL TST 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC49200.1.
RefSeqNP_770575.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89NA7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll3935.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC49200; BAC49200; BAC49200.
GeneID1053915.
KEGGbja:bll3935.
PATRIC21191372. VBIBraJap65052_3942.

Phylogenomic databases

eggNOGCOG2066.
HOGENOMHOG000216890.
KOK01425.
OrthoDBEOG6N94BK.
PhylomeDBQ89NA7.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-3960-MONOMER.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR012338. Beta-lactam/transpept-like.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR015868. Glutaminase.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF00027. cNMP_binding. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
PROSITEPS50042. CNMP_BINDING_3. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLSA1_BRADU
AccessionPrimary (citable) accession number: Q89NA7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families