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Q89NA7

- GLSA1_BRADU

UniProt

Q89NA7 - GLSA1_BRADU

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Protein

Glutaminase 1

Gene

glsA1

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751SubstrateBy similarity
Binding sitei124 – 1241SubstrateBy similarity
Binding sitei168 – 1681SubstrateBy similarity
Binding sitei175 – 1751SubstrateBy similarity
Binding sitei199 – 1991SubstrateBy similarity
Binding sitei251 – 2511SubstrateBy similarity
Binding sitei269 – 2691Substrate; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi480 – 595116cNMPAdd
BLAST

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-3960-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase 1 (EC:3.5.1.2)
Gene namesi
Name:glsA1
Ordered Locus Names:bll3935
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 613613Glutaminase 1PRO_0000110597Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi224911.bll3935.

Structurei

3D structure databases

ProteinModelPortaliQ89NA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini345 – 457113STASAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni33 – 315283GlutaminaseAdd
BLAST

Sequence similaritiesi

Belongs to the glutaminase family.Curated
Contains 1 STAS domain.Curated

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
InParanoidiQ89NA7.
KOiK01425.
OrthoDBiEOG6N94BK.
PhylomeDBiQ89NA7.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR015868. Glutaminase.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS50042. CNMP_BINDING_3. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89NA7-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MTPPPASAWT RSKPPLLRFL DTCLNEFSAE TSGAVADYIP ELGNADPAYF
60 70 80 90 100
GISLATLDGH VYEVGDSRVP FTIQSMSKPF VFALALDLLG AGRVESAIGV
110 120 130 140 150
EPSGDPFNSI RLNSDNHPFN PMVNAGAIAC TGLIYDSKGA EAFEQIRLAL
160 170 180 190 200
SRFAGRDLAV DEAVYSSESQ TGDRNRAIGY LLKTNAVISD NVAAVLDVYF
210 220 230 240 250
RQCAVLVTAR DIAVMAATLA NRGINPVTGE QVMSAYAISR TLSVMTSSGM
260 270 280 290 300
YDYAGEWIYR IGIPAKSGVG GGILAALPAR LGLGSYSPKL DKHGNSVRGI
310 320 330 340 350
KVCEALSSHY DLHMLNRSDD ARNAVIADYD IGKSPSRRVR RAQEREILAA
360 370 380 390 400
HEQEVRIIEL VGTLSLSAVD YVSRRLAGRP RPQFVIFDLH RVTSTTRAGA
410 420 430 440 450
RLVAEAFEEL AALNVTVVLS GVRRASKEWN TLREWTAELK NVRDFYLLDT
460 470 480 490 500
AIEWAEDQIV YRYGGSIDFH ETTELAEQPL LEGLSADELA ELGAICTIRT
510 520 530 540 550
YQSGAKILTT GDPADALFFL RSGAVHVTLP DGVRLATLTA GMAFGEMALL
560 570 580 590 600
EQTRSADVFA DMAATAFEAP LKDFERFREQ HPRASERIMR NLAQLLADRL
610
IVANAKVDIL TST
Length:613
Mass (Da):66,635
Last modified:June 1, 2003 - v1
Checksum:i2F7F6A35D48DA118
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC49200.1.
RefSeqiNP_770575.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC49200; BAC49200; BAC49200.
GeneIDi1053915.
KEGGibja:bll3935.
PATRICi21191372. VBIBraJap65052_3942.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC49200.1 .
RefSeqi NP_770575.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89NA7.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.bll3935.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC49200 ; BAC49200 ; BAC49200 .
GeneIDi 1053915.
KEGGi bja:bll3935.
PATRICi 21191372. VBIBraJap65052_3942.

Phylogenomic databases

eggNOGi COG2066.
HOGENOMi HOG000216890.
InParanoidi Q89NA7.
KOi K01425.
OrthoDBi EOG6N94BK.
PhylomeDBi Q89NA7.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-3960-MONOMER.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
3.40.710.10. 1 hit.
HAMAPi MF_00313. Glutaminase.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR018490. cNMP-bd-like.
IPR000595. cNMP-bd_dom.
IPR015868. Glutaminase.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
[Graphical view ]
PANTHERi PTHR12544. PTHR12544. 1 hit.
Pfami PF00027. cNMP_binding. 1 hit.
PF04960. Glutaminase. 1 hit.
[Graphical view ]
SMARTi SM00100. cNMP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
PROSITEi PS50042. CNMP_BINDING_3. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiGLSA1_BRADU
AccessioniPrimary (citable) accession number: Q89NA7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3