Reviewed, UniProtKB/Swiss-Prot Q89MZ4 (PANB3_BRAJA)

Names and origin

Protein names

Recommended name:
    3-methyl-2-oxobutanoate hydroxymethyltransferase 3
    EC=2.1.2.11
Alternative name(s):
    Ketopantoate hydroxymethyltransferase 3
      Short name=KPHMT 3

Gene names

Name:	panB3
Ordered Locus Names:	blr4048

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	273 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate. HAMAP MF_00156
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantoate from 3-methyl-2-oxobutanoate: step 1/2. HAMAP MF_00156
Subunit structure	Homodecamer; pentamer of dimers By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the panB family.

Ontologies

Keywords
Biological process	Pantothenate biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	pantothenate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-methyl-2-oxobutanoate hydroxymethyltransferase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 273	273	3-methyl-2-oxobutanoate hydroxymethyltransferase 3 HAMAP MF_00156		PRO_0000184825
Regions
	Region	49 – 50	2	Alpha-ketoisovalerate binding By similarity
Sites
	Active site	187	1	Proton acceptor By similarity
	Metal binding	49	1	Magnesium By similarity
	Metal binding	88	1	Magnesium By similarity
	Metal binding	120	1	Magnesium By similarity
	Binding site	88	1	Alpha-ketoisovalerate By similarity
	Binding site	118	1	Alpha-ketoisovalerate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89MZ4-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 8E7A57FCACACFC8F	FASTA	273	29,207
	10 20 30 40 50 60 MSVQSAIRRK TAPDLRIRKN GEPIVMLTSY HAHTAALVDR HCDAILVGDS LGNVMHGFET 70 80 90 100 110 120 TVPVTLEMMI LQGCAVMRGS SQALVVVDMP FGSYEGSKEQ AFQSAVRIMK ETLCGAVKLE 130 140 150 160 170 180 GGARMAETVA FLSERGIPVM GHIGLTPQSI NTLGSFRAQG REEANWAPIE NDARAIAEAG 190 200 210 220 230 240 AFSIVIEAVA EPLARKITQS IAVPTIGIGA SAACDGQILV LEDMLGLSPR SPKFVRRYGN 250 260 270 LGPAIEEAIA GYARDVKSRA FPGPEHVYDM KKS

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC49313.1.
RefSeq	NP_770688.1.
3D structure databases
HSSP	HSSP built from PDB template 1OY0 based on UniProtKB Q10505.
ModBase	Search...
Genome annotation databases
GeneID	1048697.
GenomeReviews	Gene locus blr4048 in contig BA000040_GR.
KEGG	bja:blr4048.
NMPDR	fig|224911.1.peg.4048.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89MZ4.
OMA	LVVVDMP.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR4048-MON.
BRENDA	2.1.2.11. 280.
Family and domain databases
HAMAP	MF_00156. [Tree]
InterPro	IPR003700. Pantoate_hydroxy_MeTrfase. IPR015813. Pyrv/PenolPyrv_Kinase_cat. [Graphical view]
Gene3D	G3DSA:3.20.20.60. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
PANTHER	PTHR20881. Pantoate_transf. 1 hit.
Pfam	PF02548. Pantoate_transf. 1 hit. [Graphical view]
PIRSF	PIRSF000388. Pantoate_hydroxy_MeTrfase. 1 hit.
TIGRFAMs	TIGR00222. panB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PANB3_BRAJA

Accession

Primary (citable) accession number: Q89MZ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)