Reviewed, UniProtKB/Swiss-Prot Q89MT4 (AMPA_BRAJA)

Names and origin

Protein names

Recommended name:
    Probable cytosol aminopeptidase
    EC=3.4.11.1
Alternative name(s):
    Leucine aminopeptidase
      Short name=LAP
    Leucyl aminopeptidase

Gene names

Name:	pepA
Ordered Locus Names:	blr4108

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides By similarity. HAMAP MF_00181
Catalytic activity	Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low. HAMAP MF_00181
Cofactor	Binds 2 manganese ions per subunit By similarity. HAMAP MF_00181
Subcellular location	Cytoplasm By similarity HAMAP MF_00181.
Sequence similarities	Belongs to the peptidase M17 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: HAMAP manganese ion binding Inferred from electronic annotation. Source: HAMAP metalloexopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Probable cytosol aminopeptidase HAMAP MF_00181		PRO_0000165727
Sites
	Active site	276	1	Potential
	Active site	350	1	Potential
	Metal binding	264	1	Manganese 2 By similarity
	Metal binding	269	1	Manganese 1 By similarity
	Metal binding	269	1	Manganese 2 By similarity
	Metal binding	287	1	Manganese 2 By similarity
	Metal binding	346	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 1 By similarity
	Metal binding	348	1	Manganese 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89MT4-1 [UniParc]. Last modified December 7, 2004. Version 2. Checksum: 44E82713197A8017	FASTA	499	51,845
	10 20 30 40 50 60 MSDAIKVGFV PLSAAARGIL VVFCDDGLKL GPATAKALGG ATELVKRAAS AAAFKGKSGA 70 80 90 100 110 120 ALDILAPEGV KATRLIVIGA GKATGLKAND FLKFGGVAAG KLSATAAAMT IMAELPDGAM 130 140 150 160 170 180 TSEQAVAIAA GLRLRAYKFD RYKTKKKDGE EGGSRADISL AVGDVAAAKK AFASAGHVVD 190 200 210 220 230 240 GVIIARDLVN EPPNVLFPEE FARRASLLRK LGVKVEVLDV KAMDKLGMGA LLGVGQGSTR 250 260 270 280 290 300 PSRTVIMRWD GGKKGEAPVA FVGKGVCFDT GGISIKPAGS MEDMKGDMGG AACVVGLMHA 310 320 330 340 350 360 LAARKAKANV VGAIGLVENM PDGNAQRPGD IVTSMSGQTI EIINTDAEGR LVLADVLWYV 370 380 390 400 410 420 AKKTKPKFMV DLATLTGAIV VALGTEHAGM FSNNDELADR LLTAGIESGE KVWRLPLGPE 430 440 450 460 470 480 YDKLIDSQFA DMKNTGGRHG GSITAAQFLQ RFVDGTPWAH LDIAGTAMGA PKTDINQSWG 490 SGYGVRLLDR LVADHYERK

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC49373.1. Different initiation.
RefSeq	NP_770748.1.
3D structure databases
SMR	Q89MT4. Positions 13-495.
ModBase	Search...
Protein family/group databases
MEROPS	M17.003.
Genome annotation databases
GeneID	1049452.
GenomeReviews	Gene locus blr4108 in contig BA000040_GR.
KEGG	bja:blr4108.
NMPDR	fig|224911.1.peg.4108.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG742580.
OMA	DSQFADM.
PhylomeDB	Q89MT4.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR4108-MONOMER.
BRENDA	3.4.11.1. 280.
Family and domain databases
HAMAP	MF_00181. Cytosol_peptidase_M17. [Tree]
InterPro	IPR011356. Peptidase_M17. IPR000819. Peptidase_M17_C. IPR008283. Peptidase_M17_N. [Graphical view]
PANTHER	PTHR11963:SF3. Peptidase_M17. 1 hit.
Pfam	PF00883. Peptidase_M17. 1 hit. PF02789. Peptidase_M17_N. 1 hit. [Graphical view]
PRINTS	PR00481. LAMNOPPTDASE.
PROSITE	PS00631. CYTOSOL_AP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMPA_BRAJA

Accession

Primary (citable) accession number: Q89MT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 7, 2004
Last sequence update:	December 7, 2004
Last modified:	January 19, 2010
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)