Reviewed, UniProtKB/Swiss-Prot Q89MP9 (SYD_BRAJA)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	blr4143

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	590 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00044
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 590	590	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_0000110840

Sequences

	Sequence		Length	Mass (Da)
	Q89MP9-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7213E9ECDB8DB7D2	FASTA	590	66,690
	10 20 30 40 50 60 MHRYRSHTCG ALRESNIGET IRLSGWVHRV RDHGGVLFID LRDHYGLTQC VVDPDSPAFS 70 80 90 100 110 120 LAEKLRSEFV IKMDGKVRRR PEGTDNDDLP TGKIEIYVSE IEVLGPAGDL PLPVFGDQEY 130 140 150 160 170 180 PEDIRLKYRF LDLRREKLHQ NIMTRVEIIK SMRRRMEGQG FFEFNTPILT ASSPEGARDF 190 200 210 220 230 240 LVPSRIHPGK FYALPQAPQQ YKQLLMMSGF DRYFQIAPCF RDEDPRADRL PGEFYQLDVE 250 260 270 280 290 300 MSFVTQEDVF AAMEPVITGV FEEFAKGKPV SKNWRRIPFA EALRKYGSDK PDLRNPIEMQ 310 320 330 340 350 360 EVSEHFRGSG FKVFARMLED PKNQVWAIPA PGGGSRAFCD RMNSWAQGEG QPGLGYIMWR 370 380 390 400 410 420 EGGEGAGPLA NNIGPERTAA IRAQIGVKEG DAAFFVAGDP DKFWKFSGLA RNKVGEELNL 430 440 450 460 470 480 TDKERFELAW IVDFPMYEYN EDDKKVDFSH NPFSMPQGGL EALKGQDPLT IKAFQYDITC 490 500 510 520 530 540 NGYEIASGGI RNHVPEAMVK AFEIAGYGEQ EVVDRFGGMY RAFQYGAPPH GGMAAGVDRI 550 560 570 580 590 VMLLCGTTNL REISLFPMNQ QAMDLLMGAP SEATTKQLRE LHVRVNLPQK

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC49408.1.
RefSeq	NP_770783.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1054376.
GenomeReviews	Gene locus blr4143 in contig BA000040_GR.
KEGG	bja:blr4143.
NMPDR	fig|224911.1.peg.4143.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89MP9.
OMA	Q89MP9. YQLDVEM.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR4143-MON.
BRENDA	6.1.1.12. 280.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR004115. GAD. IPR012340. NA-bd_OB-fold. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF02938. GAD. 1 hit. PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_BRAJA

Accession

Primary (citable) accession number: Q89MP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)