Reviewed, UniProtKB/Swiss-Prot Q89LK8 (ILVD1_BRAJA)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 1
      Short name=DAD 1
    EC=4.2.1.9

Gene names

Name:	ilvD1
Ordered Locus Names:	bll4536

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	616 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 616	616	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000103441
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	195	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89LK8-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 82ABA807FE7F5910	FASTA	616	65,288
	10 20 30 40 50 60 MPAYRSRTTT HGRNMAGARG LWRATGMKDA DFGKPIIAVV NSFTQFVPGH VHLKDLGQLV 70 80 90 100 110 120 AREIEQAGGV AKEFNTIAVD DGIAMGHDGM LYSLPSRELI ADSVEYMANA HCADGLVCIS 130 140 150 160 170 180 NCDKITPGML MAALRLNIPA VFVSGGPMEA GKVKLQGKTK AVDLIDAMVA AADSKVSDED 190 200 210 220 230 240 VKVIERSACP TCGSCSGMFT ANSMNCLTEA LGLALPGNGS VVATHADRKR LFVEAGHTIV 250 260 270 280 290 300 DLVRRYYEQD DASVLPRNVA NFKAFENAMT LDIAMGGSTN TVLHLLAAAH EGQVAFTMKD 310 320 330 340 350 360 IDRLSRRVPV LCKVAPSVAD VHVEDVHRAG GIMGILGELD RAGLIDTSVS TVHAPTMADA 370 380 390 400 410 420 LERWDIKRSK SEAVRTFYRA SPGGIPTQVA FSQERRYDEL DADREKGVVR DLEHAFSKDG 430 440 450 460 470 480 GLAVLYGNLA QDGCIVKTAG VDASILKFSG PARVFESQDA AVEGILGGKV VAGEVVVIIY 490 500 510 520 530 540 EGPRGGPGMQ EMLYPTSYLK SMGLGKACAL VTDGRFSGGS SGLSIGHLSP EAAEGGNIGL 550 560 570 580 590 600 VRTGDLIAID IPNRSITLEV SDEELAKRRA SEEAKGDAAW QATGRKRNVS TALQAYAALT 610 TSAARGAVRE VKRRSN

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC49801.1.
RefSeq	NP_771176.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1052560.
GenomeReviews	Gene locus bll4536 in contig BA000040_GR.
KEGG	bja:bll4536.
NMPDR	fig|224911.1.peg.4536.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	SRKVPCL.
PhylomeDB	Q89LK8.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL4536-MONOMER.
BRENDA	4.2.1.9. 280.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_BRAJA

Accession

Primary (citable) accession number: Q89LK8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	February 9, 2010
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)