Reviewed, UniProtKB/Swiss-Prot Q89L21 (TRMFO_BRAJA)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Synonyms:	gid
Ordered Locus Names:	blr4727

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	475 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 475	475	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000117237
Regions
	Nucleotide binding	13 – 18	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89L21-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7DE46881B1D39969	FASTA	475	50,908
	10 20 30 40 50 60 MTGPHSNIVH VIGAGLAGSE AAWQVAKAGV PVMLHEMRPD RMTEAHRTDG LAELVCSNSF 70 80 90 100 110 120 RSDDAANNAV GLLHAEMRRL GSLIMRAADA NQVPAGGALA VDRDGFSAAV TKALNDHPLI 130 140 150 160 170 180 EIARGEIAGL PPADWGNVIV ATGPLTSAPL ADAIRELTDE NALAFFDAIA PIVHRESIDM 190 200 210 220 230 240 SVAWFQSRYD KVGPGGNGAD YINCPMTKEQ YEGFVAALIA GEKTEFKEWE TNTPYFDGCL 250 260 270 280 290 300 PIEVMAERGP ETLRHGPMKP VGLTNPHDPT TKAYAIVQLR QDNKLGTLYN IVGFQTKLKY 310 320 330 340 350 360 GEQQRIFRTI PGLEKAEFAR LGGLHRNTFL NSPKLLDGQL RLRAQPRLRF AGQMTGCEGY 370 380 390 400 410 420 VESASVGLIA GLYAAADARG ETLASPPATT ALGSLLGHIT GGHIETIEPG TRSFQPMNIN 430 440 450 460 470 FGLFPPLASA PTKKPDGTRL RGNEKTVAKK QAMSALALAD LDRWIADHLR IAAAA

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC49992.1.
RefSeq	NP_771367.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1052322.
GenomeReviews	Gene locus blr4727 in contig BA000040_GR.
KEGG	bja:blr4727.
NMPDR	fig|224911.1.peg.4727.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89L21.
OMA	Q89L21. MKPVGLT.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR4727-MON.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. [Graphical view]
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_BRAJA

Accession

Primary (citable) accession number: Q89L21

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 40 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)