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Q89L21 (TRMFO_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 59. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO
EC=2.1.1.74
Alternative name(s):
Folate-dependent tRNA (uracil-5-)-methyltransferase
Folate-dependent tRNA(M-5-U54)-methyltransferase
Gene names
Name:trmFO
Synonyms:gid
Ordered Locus Names:blr4727
OrganismBradyrhizobium japonicum
Taxonomic identifier375 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length475 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity. HAMAP MF_01037

Catalytic activity

5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037

Cofactor

FAD By similarity. HAMAP MF_01037

Subcellular location

Cytoplasm By similarity HAMAP MF_01037.

Sequence similarities

Belongs to the MnmG family. TrmFO subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandFAD
Flavoprotein
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionflavin adenine dinucleotide binding

Inferred from electronic annotation. Source: InterPro

methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 475475Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO HAMAP MF_01037
PRO_0000117237

Regions

Nucleotide binding13 – 186FAD By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89L21 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 7DE46881B1D39969

FASTA47550,908
        10         20         30         40         50         60 
MTGPHSNIVH VIGAGLAGSE AAWQVAKAGV PVMLHEMRPD RMTEAHRTDG LAELVCSNSF 

        70         80         90        100        110        120 
RSDDAANNAV GLLHAEMRRL GSLIMRAADA NQVPAGGALA VDRDGFSAAV TKALNDHPLI 

       130        140        150        160        170        180 
EIARGEIAGL PPADWGNVIV ATGPLTSAPL ADAIRELTDE NALAFFDAIA PIVHRESIDM 

       190        200        210        220        230        240 
SVAWFQSRYD KVGPGGNGAD YINCPMTKEQ YEGFVAALIA GEKTEFKEWE TNTPYFDGCL 

       250        260        270        280        290        300 
PIEVMAERGP ETLRHGPMKP VGLTNPHDPT TKAYAIVQLR QDNKLGTLYN IVGFQTKLKY 

       310        320        330        340        350        360 
GEQQRIFRTI PGLEKAEFAR LGGLHRNTFL NSPKLLDGQL RLRAQPRLRF AGQMTGCEGY 

       370        380        390        400        410        420 
VESASVGLIA GLYAAADARG ETLASPPATT ALGSLLGHIT GGHIETIEPG TRSFQPMNIN 

       430        440        450        460        470 
FGLFPPLASA PTKKPDGTRL RGNEKTVAKK QAMSALALAD LDRWIADHLR IAAAA

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		BA000040 Genomic DNA. Translation: BAC49992.1.
RefSeqNP_771367.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89L21.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1052322.
GenomeReviewsGene locus blr4727 in contig BA000040_GR.
KEGGbja:blr4727.
NMPDRfig|224911.1.peg.4727.
PATRIC21193098. VBIBraJap65052_4798.

Phylogenomic databases

HOGENOMHBG285041.
OMARFAGQIT.
PhylomeDBQ89L21.
ProtClustDBPRK05335.

Enzyme and pathway databases

BioCycBJAP224911:BLR4727-MONOMER.

Family and domain databases

HAMAPMF_01037. TrmFO.
[Tree]
InterProIPR004417. Folate-dep_Ribothymidyl_synth.
IPR002218. GIDA-rel.
IPR020595. GIDA-rel_CS.
[Graphical view]
KOK04094.
PfamPF01134. GIDA. 1 hit.
[Graphical view]
TIGRFAMsTIGR00137. Gid_trmFO. 1 hit.
PROSITEPS01280. GIDA_1. False negative.
PS01281. GIDA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMFO_BRAJA
AccessionPrimary (citable) accession number: Q89L21
Entry history
Integrated into UniProtKB/Swiss-Prot: July 25, 2003
Last sequence update: June 1, 2003
Last modified: January 25, 2012
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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