Reviewed, UniProtKB/Swiss-Prot Q89KY5 (ILVD2_BRAJA)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	bll4763

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	574 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 574	574	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000103442
Sites
	Metal binding	129	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	201	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q89KY5-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A63866B173E62110	FASTA	574	60,812
	10 20 30 40 50 60 MNAKTNIKQR LPSRHVTEGP ARAPHRSYFY AMGLTTEQIH QPFVGVASCW NEAAPCNIAL 70 80 90 100 110 120 MRQAQAVKKG VAHAGGTPRE FCTITVTDGI AMGHDGMRSS LPSRECIADS VELTVRGHAY 130 140 150 160 170 180 DALVGLAGCD KSLPGMMMAM VRLNVPSIFI YGGSILPGNF RGQQVTVQDM FEAVGKHSVG 190 200 210 220 230 240 AMSDEDLDEI ERVACPSAGA CGAQFTANTM ATVSEAIGLA LPYSAGAPAP YEIRDAFCMT 250 260 270 280 290 300 AGEKVMELID QNIRPRDIVT RKALENAAAV VAASGGSTNA ALHLPAIAHE CGIKFDLFDV 310 320 330 340 350 360 AEIFKKTPYV ADLKPGGRYV AKDMFEVGGI PLLMKTLLDN GFLHGDCITV TGRTIAENLK 370 380 390 400 410 420 SVKWNPHQDV VHPADKPITV TGGVVGLKGN LAPEGAIVKV AGMSNLRFTG PARCFDREED 430 440 450 460 470 480 AFEAVQNRTY REGEVIVIRY EGPKGGPGMR EMLQTTAALT GQGMGGKIAL ITDGRFSGAT 490 500 510 520 530 540 RGFCIGHVGP EAAIGGPIGL LEDGDIIEID AVAGTLNVKL SDQELAQRKT KWSARATNHT 550 560 570 TGALWKYAQQ VGPAVGGAVT HPGGAHEKQC YADI

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC50028.1.
RefSeq	NP_771403.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1052274.
GenomeReviews	Gene locus bll4763 in contig BA000040_GR.
KEGG	bja:bll4763.
NMPDR	fig|224911.1.peg.4763.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89KY5.
OMA	MMMAMVR.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL4763-MON.
BRENDA	4.2.1.9. 280.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_BRAJA

Accession

Primary (citable) accession number: Q89KY5

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)