Q89KV2 (GLSA2_BRAJA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutaminase 2
EC=3.5.1.2

Gene names

Name:	glsA2
Ordered Locus Names:	bll4798

Organism

Bradyrhizobium japonicum (strain USDA 110) [Reference proteome] [HAMAP]

Taxonomic identifier

224911 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium › Bradyrhizobium japonicum

Protein attributes

Sequence length	624 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the glutaminase family. Contains 1 cyclic nucleotide-binding domain. Contains 1 STAS domain.

Ontologies

Keywords
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glutamine metabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	glutaminase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 624	624	Glutaminase 2 HAMAP-Rule MF_00313		PRO_0000110598
Regions
	Domain	355 – 466	112	STAS
	Nucleotide binding	491 – 608	118	cNMP HAMAP-Rule MF_00313
	Region	43 – 325	283	Glutaminase HAMAP-Rule MF_00313
Sites
	Binding site	85	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	178	1	Substrate By similarity
	Binding site	185	1	Substrate By similarity
	Binding site	209	1	Substrate By similarity
	Binding site	261	1	Substrate By similarity
	Binding site	279	1	Substrate; via amide nitrogen By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89KV2 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 75646994C0FF9DAC	FASTA	624	67,583
	10 20 30 40 50 60 MDTQPIRLPS VAGATRSAGY PTRPPLRRFL TDCHEEFRGD SSGELADYIP ELKRANPDHF 70 80 90 100 110 120 GIALVTIDGH VYEVGDSAVP FTIQSVSKAF VFALALETVG EERVSATIGV EPSGEAFNSI 130 140 150 160 170 180 RLTNDNRPFN PMVNAGAIAC SGLIYEVDGK GAFERVRSKL SEFAGRELGV DEAVHASETA 190 200 210 220 230 240 TGNRNRAIAW LLRNYAVLPD DVDAVLDVYF RQCAILVTAR DLAVMAATLA NRGINPVTGA 250 260 270 280 290 300 QVITPHIVAR TLSVMTSSGM YDYAGEWTYR VGIPAKSGVG GGIVAALPSQ LGLGTFSPLL 310 320 330 340 350 360 DNHFNSVRGL KVCEALSARF DLHMLNRNAD VRTSVMADYD VYGISSRRSR QPHEQQILDE 370 380 390 400 410 420 RHSDIRIVEL VGALNFGTID YVTRRLTSEP PNAPLLIIDF RRVPDITAAG AELLGETLTA 430 440 450 460 470 480 LGNANVTTIL SGLEEASAVW AAIAARTGDP RRLRRFALLD DAIEWAEDQV IYRFGGFTDV 490 500 510 520 530 540 KESVHLGEQA LLAELDTDEI AAIVKLSTTR HYTAGQRVIA AGAPANSLFF LQSGMVSVKL 550 560 570 580 590 600 RSGVRLASLG PGMEFGEMAI LERTRSADVF ADTPVACLEL PLDSFADYRR LHPETALKIM 610 620 RNLAAILARR LVAANAKVDL LSAY

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000040 Genomic DNA. Translation: BAC50063.1.
RefSeq	NP_771438.1. NC_004463.1.
3D structure databases
ProteinModelPortal	Q89KV2.
ModBase	Search...
Protein-protein interaction databases
STRING	224911.bll4798.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC50063; BAC50063; BAC50063.
GeneID	1052223.
KEGG	bja:bll4798.
PATRIC	21193254. VBIBraJap65052_4876.
Phylogenomic databases
eggNOG	COG2066.
HOGENOM	HOG000216890.
KO	K01425.
OMA	SEFAGRE.
ProtClustDB	CLSK911128.
Enzyme and pathway databases
BioCyc	BJAP224911:GJEJ-4831-MONOMER.
Family and domain databases
Gene3D	2.60.120.10. 1 hit. 3.30.750.24. 1 hit. 3.40.710.10. 1 hit.
HAMAP	MF_00313. Glutaminase. Fused.
InterPro	IPR012338. Beta-lactam/transpept-like. IPR018490. cNMP-bd-like. IPR018488. cNMP-bd_CS. IPR000595. cNMP-bd_dom. IPR015868. Glutaminase. IPR014710. RmlC-like_jellyroll. IPR002645. STAS_dom. [Graphical view]
PANTHER	PTHR12544. PTHR12544. 1 hit.
Pfam	PF00027. cNMP_binding. 1 hit. PF04960. Glutaminase. 1 hit. PF01740. STAS. 1 hit. [Graphical view]
SMART	SM00100. cNMP. 1 hit. [Graphical view]
SUPFAM	SSF51206. cNMP_binding. 1 hit. SSF56601. PBP_transp_fold. 1 hit. SSF52091. STAS. 1 hit.
TIGRFAMs	TIGR03814. Gln_ase. 1 hit.
PROSITE	PS00888. CNMP_BINDING_1. 1 hit. PS50042. CNMP_BINDING_3. 1 hit. PS50801. STAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLSA2_BRAJA

Accession

Primary (citable) accession number: Q89KV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 24, 2004
Last sequence update:	June 1, 2003
Last modified:	May 1, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program