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Q89KV2

- GLSA2_BRADU

UniProt

Q89KV2 - GLSA2_BRADU

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Protein

Glutaminase 2

Gene

glsA2

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalytic activityi

L-glutamine + H2O = L-glutamate + NH3.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei85 – 851SubstrateBy similarity
Binding sitei134 – 1341SubstrateBy similarity
Binding sitei178 – 1781SubstrateBy similarity
Binding sitei185 – 1851SubstrateBy similarity
Binding sitei209 – 2091SubstrateBy similarity
Binding sitei261 – 2611SubstrateBy similarity
Binding sitei279 – 2791Substrate; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi491 – 608118cNMPAdd
BLAST

GO - Molecular functioni

  1. glutaminase activity Source: UniProtKB-EC

GO - Biological processi

  1. glutamine metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-4831-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaminase 2 (EC:3.5.1.2)
Gene namesi
Name:glsA2
Ordered Locus Names:bll4798
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 624624Glutaminase 2PRO_0000110598Add
BLAST

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi224911.bll4798.

Structurei

3D structure databases

ProteinModelPortaliQ89KV2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini355 – 466112STASAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni43 – 325283GlutaminaseAdd
BLAST

Sequence similaritiesi

Belongs to the glutaminase family.Curated
Contains 1 STAS domain.Curated

Phylogenomic databases

eggNOGiCOG2066.
HOGENOMiHOG000216890.
InParanoidiQ89KV2.
KOiK01425.
OrthoDBiEOG6N94BK.
PhylomeDBiQ89KV2.

Family and domain databases

Gene3Di2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
3.40.710.10. 1 hit.
HAMAPiMF_00313. Glutaminase.
InterProiIPR012338. Beta-lactam/transpept-like.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR015868. Glutaminase.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
[Graphical view]
PANTHERiPTHR12544. PTHR12544. 1 hit.
PfamiPF00027. cNMP_binding. 1 hit.
PF04960. Glutaminase. 1 hit.
PF01740. STAS. 1 hit.
[Graphical view]
SMARTiSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMiSSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsiTIGR03814. Gln_ase. 1 hit.
PROSITEiPS00888. CNMP_BINDING_1. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89KV2-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDTQPIRLPS VAGATRSAGY PTRPPLRRFL TDCHEEFRGD SSGELADYIP
60 70 80 90 100
ELKRANPDHF GIALVTIDGH VYEVGDSAVP FTIQSVSKAF VFALALETVG
110 120 130 140 150
EERVSATIGV EPSGEAFNSI RLTNDNRPFN PMVNAGAIAC SGLIYEVDGK
160 170 180 190 200
GAFERVRSKL SEFAGRELGV DEAVHASETA TGNRNRAIAW LLRNYAVLPD
210 220 230 240 250
DVDAVLDVYF RQCAILVTAR DLAVMAATLA NRGINPVTGA QVITPHIVAR
260 270 280 290 300
TLSVMTSSGM YDYAGEWTYR VGIPAKSGVG GGIVAALPSQ LGLGTFSPLL
310 320 330 340 350
DNHFNSVRGL KVCEALSARF DLHMLNRNAD VRTSVMADYD VYGISSRRSR
360 370 380 390 400
QPHEQQILDE RHSDIRIVEL VGALNFGTID YVTRRLTSEP PNAPLLIIDF
410 420 430 440 450
RRVPDITAAG AELLGETLTA LGNANVTTIL SGLEEASAVW AAIAARTGDP
460 470 480 490 500
RRLRRFALLD DAIEWAEDQV IYRFGGFTDV KESVHLGEQA LLAELDTDEI
510 520 530 540 550
AAIVKLSTTR HYTAGQRVIA AGAPANSLFF LQSGMVSVKL RSGVRLASLG
560 570 580 590 600
PGMEFGEMAI LERTRSADVF ADTPVACLEL PLDSFADYRR LHPETALKIM
610 620
RNLAAILARR LVAANAKVDL LSAY
Length:624
Mass (Da):67,583
Last modified:June 1, 2003 - v1
Checksum:i75646994C0FF9DAC
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC50063.1.
RefSeqiNP_771438.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC50063; BAC50063; BAC50063.
GeneIDi1052223.
KEGGibja:bll4798.
PATRICi21193254. VBIBraJap65052_4876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC50063.1 .
RefSeqi NP_771438.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89KV2.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.bll4798.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC50063 ; BAC50063 ; BAC50063 .
GeneIDi 1052223.
KEGGi bja:bll4798.
PATRICi 21193254. VBIBraJap65052_4876.

Phylogenomic databases

eggNOGi COG2066.
HOGENOMi HOG000216890.
InParanoidi Q89KV2.
KOi K01425.
OrthoDBi EOG6N94BK.
PhylomeDBi Q89KV2.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-4831-MONOMER.

Family and domain databases

Gene3Di 2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
3.40.710.10. 1 hit.
HAMAPi MF_00313. Glutaminase.
InterProi IPR012338. Beta-lactam/transpept-like.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR015868. Glutaminase.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
[Graphical view ]
PANTHERi PTHR12544. PTHR12544. 1 hit.
Pfami PF00027. cNMP_binding. 1 hit.
PF04960. Glutaminase. 1 hit.
PF01740. STAS. 1 hit.
[Graphical view ]
SMARTi SM00100. cNMP. 1 hit.
[Graphical view ]
SUPFAMi SSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsi TIGR03814. Gln_ase. 1 hit.
PROSITEi PS00888. CNMP_BINDING_1. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiGLSA2_BRADU
AccessioniPrimary (citable) accession number: Q89KV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 67 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3