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Q89KV2 (GLSA2_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutaminase 2

EC=3.5.1.2
Gene names
Name:glsA2
Ordered Locus Names:bll4798
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length624 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

L-glutamine + H2O = L-glutamate + NH3. HAMAP-Rule MF_00313

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00313

Sequence similarities

Belongs to the glutaminase family.

Contains 1 cyclic nucleotide-binding domain.

Contains 1 STAS domain.

Ontologies

Keywords
   Molecular functionHydrolase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutamine metabolic process

Inferred from electronic annotation. Source: InterPro

   Molecular_functionglutaminase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 624624Glutaminase 2 HAMAP-Rule MF_00313
PRO_0000110598

Regions

Domain355 – 466112STAS
Nucleotide binding491 – 608118cNMP HAMAP-Rule MF_00313
Region43 – 325283Glutaminase HAMAP-Rule MF_00313

Sites

Binding site851Substrate By similarity
Binding site1341Substrate By similarity
Binding site1781Substrate By similarity
Binding site1851Substrate By similarity
Binding site2091Substrate By similarity
Binding site2611Substrate By similarity
Binding site2791Substrate; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89KV2 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 75646994C0FF9DAC

FASTA62467,583
        10         20         30         40         50         60 
MDTQPIRLPS VAGATRSAGY PTRPPLRRFL TDCHEEFRGD SSGELADYIP ELKRANPDHF 

        70         80         90        100        110        120 
GIALVTIDGH VYEVGDSAVP FTIQSVSKAF VFALALETVG EERVSATIGV EPSGEAFNSI 

       130        140        150        160        170        180 
RLTNDNRPFN PMVNAGAIAC SGLIYEVDGK GAFERVRSKL SEFAGRELGV DEAVHASETA 

       190        200        210        220        230        240 
TGNRNRAIAW LLRNYAVLPD DVDAVLDVYF RQCAILVTAR DLAVMAATLA NRGINPVTGA 

       250        260        270        280        290        300 
QVITPHIVAR TLSVMTSSGM YDYAGEWTYR VGIPAKSGVG GGIVAALPSQ LGLGTFSPLL 

       310        320        330        340        350        360 
DNHFNSVRGL KVCEALSARF DLHMLNRNAD VRTSVMADYD VYGISSRRSR QPHEQQILDE 

       370        380        390        400        410        420 
RHSDIRIVEL VGALNFGTID YVTRRLTSEP PNAPLLIIDF RRVPDITAAG AELLGETLTA 

       430        440        450        460        470        480 
LGNANVTTIL SGLEEASAVW AAIAARTGDP RRLRRFALLD DAIEWAEDQV IYRFGGFTDV 

       490        500        510        520        530        540 
KESVHLGEQA LLAELDTDEI AAIVKLSTTR HYTAGQRVIA AGAPANSLFF LQSGMVSVKL 

       550        560        570        580        590        600 
RSGVRLASLG PGMEFGEMAI LERTRSADVF ADTPVACLEL PLDSFADYRR LHPETALKIM 

       610        620 
RNLAAILARR LVAANAKVDL LSAY 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC50063.1.
RefSeqNP_771438.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89KV2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll4798.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC50063; BAC50063; BAC50063.
GeneID1052223.
KEGGbja:bll4798.
PATRIC21193254. VBIBraJap65052_4876.

Phylogenomic databases

eggNOGCOG2066.
HOGENOMHOG000216890.
KOK01425.
OrthoDBEOG6N94BK.
PhylomeDBQ89KV2.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-4831-MONOMER.

Family and domain databases

Gene3D2.60.120.10. 1 hit.
3.30.750.24. 1 hit.
3.40.710.10. 1 hit.
HAMAPMF_00313. Glutaminase.
InterProIPR012338. Beta-lactam/transpept-like.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR015868. Glutaminase.
IPR014710. RmlC-like_jellyroll.
IPR002645. STAS_dom.
[Graphical view]
PANTHERPTHR12544. PTHR12544. 1 hit.
PfamPF00027. cNMP_binding. 1 hit.
PF04960. Glutaminase. 1 hit.
PF01740. STAS. 1 hit.
[Graphical view]
SMARTSM00100. cNMP. 1 hit.
[Graphical view]
SUPFAMSSF51206. SSF51206. 1 hit.
SSF52091. SSF52091. 1 hit.
SSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR03814. Gln_ase. 1 hit.
PROSITEPS00888. CNMP_BINDING_1. 1 hit.
PS50042. CNMP_BINDING_3. 1 hit.
PS50801. STAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameGLSA2_BRADU
AccessionPrimary (citable) accession number: Q89KV2
Entry history
Integrated into UniProtKB/Swiss-Prot: May 24, 2004
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families