Reviewed, UniProtKB/Swiss-Prot Q89KV0 (KDSA_BRAJA)

Names and origin

Protein names

Recommended name:
    2-dehydro-3-deoxyphosphooctonate aldolase
    EC=2.5.1.55
Alternative name(s):
    Phospho-2-dehydro-3-deoxyoctonate aldolase
    3-deoxy-D-manno-octulosonic acid 8-phosphate synthetase
    KDO-8-phosphate synthetase
      Short name=KDO 8-P synthase
      Short name=KDOPS

Gene names

Name:	kdsA
Ordered Locus Names:	bll4800

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	282 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + D-arabinose 5-phosphate + H2O = 2-dehydro-3-deoxy-D-octonate 8-phosphate + phosphate. HAMAP MF_00056
Pathway	Carbohydrate biosynthesis; 3-deoxy-D-manno-octulosonate biosynthesis; 3-deoxy-D-manno-octulosonate from D-ribulose 5-phosphate: step 2/3. HAMAP MF_00056 Bacterial outer membrane biogenesis; lipopolysaccharide biosynthesis. HAMAP MF_00056
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the kdsA family.

Ontologies

Keywords
Biological process	Lipopolysaccharide biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-deoxy-8-phosphooctulonate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 282	282	2-dehydro-3-deoxyphosphooctonate aldolase HAMAP MF_00056		PRO_0000187106

Sequences

	Sequence		Length	Mass (Da)
	Q89KV0-1 [UniParc]. Last modified June 20, 2003. Version 1. Checksum: E2115D23CD34D500	FASTA	282	29,578
	10 20 30 40 50 60 MSSSTSAAPV VTIGRVKFGN DLPISIIAGP CQLESRQHAL EVASALKEIA ARLNIGLVYK 70 80 90 100 110 120 TSFDKANRTS ASAARGLGLA QSLPIFAEIR SSLGLPVLTD VHEATQCAEV AQAVDILQIP 130 140 150 160 170 180 AFLCRQTDLL LAAAATGKVV NVKKGQFLAP WDMANVVTKI TSANNPNVLV TERGASFGYN 190 200 210 220 230 240 TLVSDMRALP ILARTTGAPV IFDATHSVQQ PGGKGTSSGG EREFVPVLAR AAVAVGVAGV 250 260 270 280 FIETHPDPDS APSDGPNMVP LREFEGLIRR LMAFDALAKN PR

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC50065.1.
RefSeq	NP_771440.1.
3D structure databases
HSSP	HSSP built from PDB template 1D9E based on UniProtKB P17579.
ModBase	Search...
Genome annotation databases
GeneID	1052221.
GenomeReviews	Gene locus bll4800 in contig BA000040_GR.
KEGG	bja:bll4800.
NMPDR	fig|224911.1.peg.4800.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89KV0.
OMA	VYKTSFD.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL4800-MON.
BRENDA	2.5.1.55. 280.
Family and domain databases
HAMAP	MF_00056. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR006218. DAHP1/KDSA. IPR006269. KDO8P_synthase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21057:SF2. KDO8P_synthase. 1 hit.
Pfam	PF00793. DAHP_synth_1. 1 hit. [Graphical view]
TIGRFAMs	TIGR01362. KDO8P_synth. 1 hit.
ProtoNet	Search...

Entry information

Entry name

KDSA_BRAJA

Accession

Primary (citable) accession number: Q89KV0

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	June 20, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)