Reviewed, UniProtKB/Swiss-Prot Q89KU3 (TPIS_BRAJA)

Names and origin

Protein names

Recommended name:
    Triosephosphate isomerase
      Short name=TIM
    EC=5.3.1.1
Alternative name(s):
    Triose-phosphate isomerase

Gene names

Name:	tpiA
Ordered Locus Names:	bll4807

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	251 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate = glycerone phosphate. HAMAP MF_00147
Pathway	Carbohydrate biosynthesis; gluconeogenesis. HAMAP MF_00147 Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate from glycerone phosphate: step 1/1. HAMAP MF_00147
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the triosephosphate isomerase family.

Ontologies

Keywords
Biological process	Gluconeogenesis Glycolysis Pentose shunt
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	gluconeogenesis Inferred from electronic annotation. Source: HAMAP glycolysis Inferred from electronic annotation. Source: HAMAP pentose-phosphate shunt Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	triose-phosphate isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 251	251	Triosephosphate isomerase HAMAP MF_00147		PRO_0000090187
Sites
	Active site	98	1	Electrophile By similarity
	Active site	168	1	Proton acceptor By similarity
	Binding site	12	1	Substrate By similarity
	Binding site	14	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89KU3-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: E4868D22097B56F9	FASTA	251	25,769
	10 20 30 40 50 60 MTDAIRPLIA GNWKMNGLKA SAAEFDAMLN GAADVATKAD LLVCPPATLI AAFADKARGK 70 80 90 100 110 120 KVAVGAQDCH PKASGAHTGD IAAEMLANAG ATAIIVGHSE RRADHGEGDA LVRQKAEAAW 130 140 150 160 170 180 RAGVTAIVCI GETQAQRDAG QTLDILRGQL DGSLPDGSTA ANLVVAYEPV WAIGTGLTPT 190 200 210 220 230 240 VQDVEQIHGF IREFLTSRFS VDGAKMRILY GGSVKPSNAA ELMAVKNVNG ALVGGASLKA 250 ADFLAIAKGC P

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC50072.1.
RefSeq	NP_771447.1.
3D structure databases
HSSP	HSSP built from PDB template 1NEY based on UniProtKB P00942.
ModBase	Search...
Genome annotation databases
GeneID	1052213.
GenomeReviews	Gene locus bll4807 in contig BA000040_GR.
KEGG	bja:bll4807.
NMPDR	fig|224911.1.peg.4807.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89KU3.
OMA	Q89KU3. IGAQDCH.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL4807-MON.
BRENDA	5.3.1.1. 280.
Family and domain databases
HAMAP	MF_00147. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR000652. Triosephosphate_isomerase. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR21139. Triophos_ismrse. 1 hit.
Pfam	PF00121. TIM. 1 hit. [Graphical view]
ProDom	PD001005. Triophos_ismrse. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00419. tim. 1 hit.
PROSITE	PS00171. TIM_1. 1 hit. PS51440. TIM_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPIS_BRAJA

Accession

Primary (citable) accession number: Q89KU3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)