Reviewed, UniProtKB/Swiss-Prot Q89KS7 (LEXA_BRAJA)

Names and origin

Protein names

Recommended name:
    LexA repressor
    EC=3.4.21.88

Gene names

Name:	lexA
Ordered Locus Names:	blr4826

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity.
Catalytic activity	Hydrolysis of Ala-|-Gly bond in repressor lexA. HAMAP MF_00015
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the peptidase S24 family.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication SOS response Transcription Transcription regulation
Ligand	DNA-binding
Molecular function	Hydrolase Repressor
PTM	Autocatalytic cleavage
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: HAMAP DNA replication Inferred from electronic annotation. Source: HAMAP SOS response Inferred from electronic annotation. Source: HAMAP negative regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: HAMAP proteolysis Inferred from electronic annotation. Source: InterPro transcription Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA binding Inferred from electronic annotation. Source: HAMAP serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	LexA repressor HAMAP MF_00015		PRO_0000170017
Regions
	DNA binding	26 – 46	21	H-T-H motif By similarity
Sites
	Active site	152	1	For autocatalytic cleavage activity By similarity
	Active site	190	1	For autocatalytic cleavage activity By similarity
	Site	117 – 118	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89KS7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: A482D4D8CA596122	FASTA	231	25,438
	10 20 30 40 50 60 MLTRKQYELL RFISERLKES GVPPSFDEMK DALDLRSKSG IHRLITALEE RGFIRRLPNR 70 80 90 100 110 120 ARAIEVIKLP ELQAAAGNRR GFTPSVIEGN LGKVRASSSA DEGERPVAVP VMGRIAAGTP 130 140 150 160 170 180 IEALQTRSHT ISVPPDMLGS GEHYALEVRG DSMVEAGILD GDMALIQRNE SADTGDIVVA 190 200 210 220 230 LIDDEEATLK RFRRRGASIA LEPANAAYEV RILPPNRVKI QGKLIGLYRK Y

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC50091.1.
RefSeq	NP_771466.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	S24.001.
Genome annotation databases
GeneID	1052157.
GenomeReviews	Gene locus blr4826 in contig BA000040_GR.
KEGG	bja:blr4826.
NMPDR	fig|224911.1.peg.4826.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89KS7.
OMA	KVIGVFR.
Enzyme and pathway databases
BioCyc	BJAP224911:BLR4826-MON.
BRENDA	3.4.21.88. 280.
Family and domain databases
HAMAP	MF_00015. [Tree]
InterPro	IPR006199. LexA_DNA_bd. IPR006200. Pept_S24_LexA. IPR006197. Peptidase_S24_LexA_cons-reg. IPR019759. Peptidase_S24_S26_cons-reg. IPR011056. Peptidase_S24_S26A/B/C_b-rbn. IPR011991. Wing_hlx_DNA_bd. [Graphical view]
Gene3D	G3DSA:2.10.109.10. Pept_S24_S26_C. 1 hit. G3DSA:1.10.10.10. Wing_hlx_DNA_bd. 1 hit.
Pfam	PF01726. LexA_DNA_bind. 1 hit. PF00717. Peptidase_S24. 1 hit. [Graphical view]
PRINTS	PR00726. LEXASERPTASE.
TIGRFAMs	TIGR00498. lexA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LEXA_BRAJA

Accession

Primary (citable) accession number: Q89KS7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 1, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)