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Q89KS7 (LEXA_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 72. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
LexA repressor

EC=3.4.21.88
Gene names
Name:lexA
Ordered Locus Names:blr4826
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length231 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, RecA interacts with LexA causing an autocatalytic cleavage which disrupts the DNA-binding part of LexA, leading to derepression of the SOS regulon and eventually DNA repair By similarity. HAMAP-Rule MF_00015

Catalytic activity

Hydrolysis of Ala-|-Gly bond in repressor LexA. HAMAP-Rule MF_00015

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00015

Sequence similarities

Belongs to the peptidase S24 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 231231LexA repressor HAMAP-Rule MF_00015
PRO_0000170017

Regions

DNA binding26 – 4621H-T-H motif By similarity

Sites

Active site1521For autocatalytic cleavage activity By similarity
Active site1901For autocatalytic cleavage activity By similarity
Site117 – 1182Cleavage; by autolysis By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89KS7 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: A482D4D8CA596122

FASTA23125,438
        10         20         30         40         50         60 
MLTRKQYELL RFISERLKES GVPPSFDEMK DALDLRSKSG IHRLITALEE RGFIRRLPNR 

        70         80         90        100        110        120 
ARAIEVIKLP ELQAAAGNRR GFTPSVIEGN LGKVRASSSA DEGERPVAVP VMGRIAAGTP 

       130        140        150        160        170        180 
IEALQTRSHT ISVPPDMLGS GEHYALEVRG DSMVEAGILD GDMALIQRNE SADTGDIVVA 

       190        200        210        220        230 
LIDDEEATLK RFRRRGASIA LEPANAAYEV RILPPNRVKI QGKLIGLYRK Y 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC50091.1.
RefSeqNP_771466.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89KS7.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.blr4826.

Protein family/group databases

MEROPSS24.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC50091; BAC50091; BAC50091.
GeneID1052157.
KEGGbja:blr4826.
PATRIC21193314. VBIBraJap65052_4906.

Phylogenomic databases

eggNOGCOG1974.
HOGENOMHOG000232168.
KOK01356.
OMAQNTANNG.
OrthoDBEOG6JHRHJ.
ProtClustDBPRK00215.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-4859-MONOMER.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
2.10.109.10. 1 hit.
HAMAPMF_00015. LexA.
InterProIPR006200. LexA.
IPR006199. LexA_DNA-bd_dom.
IPR028360. Peptidase_S24/S26_b-rbn.
IPR006197. Peptidase_S24_LexA.
IPR019759. Peptidase_S24_S26.
IPR015927. Peptidase_S24_S26A/B/C.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PfamPF01726. LexA_DNA_bind. 1 hit.
PF00717. Peptidase_S24. 1 hit.
[Graphical view]
PRINTSPR00726. LEXASERPTASE.
SUPFAMSSF51306. SSF51306. 1 hit.
TIGRFAMsTIGR00498. lexA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLEXA_BRADU
AccessionPrimary (citable) accession number: Q89KS7
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: June 1, 2003
Last modified: February 19, 2014
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries