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Q89KR6

- SYQ_BRADU

UniProt

Q89KR6 - SYQ_BRADU

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Protein

Glutamine--tRNA ligase

Gene
glnS, bll4837
Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei273 – 2731ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. glutaminyl-tRNA aminoacylation Source: InterPro
  2. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-4870-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamine--tRNA ligase (EC:6.1.1.18)
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name:
GlnRS
Gene namesi
Name:glnS
Ordered Locus Names:bll4837
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 558558Glutamine--tRNA ligaseUniRule annotationPRO_0000195826Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi224911.bll4837.

Structurei

3D structure databases

ProteinModelPortaliQ89KR6.
SMRiQ89KR6. Positions 12-550.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi36 – 4611"HIGH" regionUniRule annotationAdd
BLAST
Motifi270 – 2745"KMSKS" regionUniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000259232.
KOiK01886.
OrthoDBiEOG6DRPF7.
PhylomeDBiQ89KR6.

Family and domain databases

Gene3Di1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPiMF_00126. Gln_tRNA_synth.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF50715. SSF50715. 1 hit.
TIGRFAMsiTIGR00440. glnS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89KR6-1 [UniParc]FASTAAdd to Basket

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MTEPVAAEVG RDFIRDIIQA DLDQAKYREI VTRFPPEPNG YLHIGHAKSI    50
ALNFGIAQEF PGRCHLRFDD TNPVKEEQEY IDSIQADVHW LGFDWGKNLF 100
FASDYFDRLY EWAETLIRDG LAYVDDQTQE EIRTSRGTLT EPGKNSPFRD 150
RSVDENLDLF RRMKAGEFPN GARVLRAKID MAAGNINLRD PVLYRILHAH 200
HPRTGNAWCI YPSYDYAHGQ SDAIEGITHS ICTLEFEDHR PLYDWFIEKL 250
PVPSKPHQYE FARLNLTYTL LSKRVLTQLV REGHVAGWDD PRMPTMAGMR 300
RRGVPPAALR EFVKRIGVAK ANSVVDVGML EFCIREELNR TAQRRMAVLK 350
PLKVVIENYP EGQTEELEAI NHPDDPSAGT RKITFGRELY IEQDDFMENP 400
PKKFFRLSPG NEVRLRYAYF VKCTGVIKNE SGEVVELRCT YDPATRGGNA 450
PDGRKVKATM HWLSAAASKP AEIRIYNQLF ANPSPDASNF AADLNPQSLE 500
ILSNARVEAS VAESNSTEPM QFERQGYFVR DKDSTPGKPV FSRTIGLRDT 550
FAKEVAKG 558
Length:558
Mass (Da):63,536
Last modified:June 1, 2003 - v1
Checksum:i71CA2AE1A8440845
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC50102.1.
RefSeqiNP_771477.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC50102; BAC50102; BAC50102.
GeneIDi1052143.
KEGGibja:bll4837.
PATRICi21193334. VBIBraJap65052_4916.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000040 Genomic DNA. Translation: BAC50102.1 .
RefSeqi NP_771477.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89KR6.
SMRi Q89KR6. Positions 12-550.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.bll4837.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC50102 ; BAC50102 ; BAC50102 .
GeneIDi 1052143.
KEGGi bja:bll4837.
PATRICi 21193334. VBIBraJap65052_4916.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000259232.
KOi K01886.
OrthoDBi EOG6DRPF7.
PhylomeDBi Q89KR6.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-4870-MONOMER.

Family and domain databases

Gene3Di 1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPi MF_00126. Gln_tRNA_synth.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF50715. SSF50715. 1 hit.
TIGRFAMsi TIGR00440. glnS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiSYQ_BRADU
AccessioniPrimary (citable) accession number: Q89KR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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