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Q89KR6 (SYQ_BRADU) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glutamine--tRNA ligase

EC=6.1.1.18
Alternative name(s):
Glutaminyl-tRNA synthetase
Short name=GlnRS
Gene names
Name:glnS
Ordered Locus Names:bll4837
OrganismBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110) [Reference proteome] [HAMAP]
Taxonomic identifier224911 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium

Protein attributes

Sequence length558 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP-Rule MF_00126

Subunit structure

Monomer By similarity. HAMAP-Rule MF_00126

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00126.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processglutaminyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

glutamyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

glutamine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 558558Glutamine--tRNA ligase HAMAP-Rule MF_00126
PRO_0000195826

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00126
Motif270 – 2745"KMSKS" region HAMAP-Rule MF_00126

Sites

Binding site2731ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q89KR6 [UniParc].

Last modified June 1, 2003. Version 1.
Checksum: 71CA2AE1A8440845

FASTA55863,536
        10         20         30         40         50         60 
MTEPVAAEVG RDFIRDIIQA DLDQAKYREI VTRFPPEPNG YLHIGHAKSI ALNFGIAQEF 

        70         80         90        100        110        120 
PGRCHLRFDD TNPVKEEQEY IDSIQADVHW LGFDWGKNLF FASDYFDRLY EWAETLIRDG 

       130        140        150        160        170        180 
LAYVDDQTQE EIRTSRGTLT EPGKNSPFRD RSVDENLDLF RRMKAGEFPN GARVLRAKID 

       190        200        210        220        230        240 
MAAGNINLRD PVLYRILHAH HPRTGNAWCI YPSYDYAHGQ SDAIEGITHS ICTLEFEDHR 

       250        260        270        280        290        300 
PLYDWFIEKL PVPSKPHQYE FARLNLTYTL LSKRVLTQLV REGHVAGWDD PRMPTMAGMR 

       310        320        330        340        350        360 
RRGVPPAALR EFVKRIGVAK ANSVVDVGML EFCIREELNR TAQRRMAVLK PLKVVIENYP 

       370        380        390        400        410        420 
EGQTEELEAI NHPDDPSAGT RKITFGRELY IEQDDFMENP PKKFFRLSPG NEVRLRYAYF 

       430        440        450        460        470        480 
VKCTGVIKNE SGEVVELRCT YDPATRGGNA PDGRKVKATM HWLSAAASKP AEIRIYNQLF 

       490        500        510        520        530        540 
ANPSPDASNF AADLNPQSLE ILSNARVEAS VAESNSTEPM QFERQGYFVR DKDSTPGKPV 

       550 
FSRTIGLRDT FAKEVAKG 

« Hide

References

[1]"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110."
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.
DNA Res. 9:189-197(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000040 Genomic DNA. Translation: BAC50102.1.
RefSeqNP_771477.1. NC_004463.1.

3D structure databases

ProteinModelPortalQ89KR6.
SMRQ89KR6. Positions 12-550.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING224911.bll4837.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC50102; BAC50102; BAC50102.
GeneID1052143.
KEGGbja:bll4837.
PATRIC21193334. VBIBraJap65052_4916.

Phylogenomic databases

eggNOGCOG0008.
HOGENOMHOG000259232.
KOK01886.
OrthoDBEOG6DRPF7.
PhylomeDBQ89KR6.

Enzyme and pathway databases

BioCycBJAP224911:GJEJ-4870-MONOMER.

Family and domain databases

Gene3D1.10.1160.10. 1 hit.
2.40.240.10. 2 hits.
3.40.50.620. 2 hits.
HAMAPMF_00126. Gln_tRNA_synth.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR004514. Gln-tRNA-synth.
IPR022861. Gln_tRNA_ligase_bac.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR020059. Glu/Gln-tRNA-synth_Ib_codon-bd.
IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl.
IPR011035. Ribosomal_L25/Gln-tRNA_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERPTHR10119. PTHR10119. 1 hit.
PfamPF00749. tRNA-synt_1c. 1 hit.
PF03950. tRNA-synt_1c_C. 1 hit.
[Graphical view]
PRINTSPR00987. TRNASYNTHGLU.
SUPFAMSSF50715. SSF50715. 1 hit.
TIGRFAMsTIGR00440. glnS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYQ_BRADU
AccessionPrimary (citable) accession number: Q89KR6
Entry history
Integrated into UniProtKB/Swiss-Prot: April 26, 2004
Last sequence update: June 1, 2003
Last modified: July 9, 2014
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries