Reviewed, UniProtKB/Swiss-Prot Q89KR6 (SYQ_BRAJA)

Names and origin

Protein names

Recommended name:
    Glutaminyl-tRNA synthetase
    EC=6.1.1.18
Alternative name(s):
    Glutamine--tRNA ligase
      Short name=GlnRS

Gene names

Name:	glnS
Ordered Locus Names:	bll4837

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln). HAMAP MF_00126
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00126
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutaminyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Glutaminyl-tRNA synthetase HAMAP MF_00126		PRO_0000195826
Regions
	Motif	36 – 46	11	"HIGH" region HAMAP MF_00126
	Motif	270 – 274	5	"KMSKS" region HAMAP MF_00126
Sites
	Binding site	273	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89KR6-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 71CA2AE1A8440845	FASTA	558	63,536
	10 20 30 40 50 60 MTEPVAAEVG RDFIRDIIQA DLDQAKYREI VTRFPPEPNG YLHIGHAKSI ALNFGIAQEF 70 80 90 100 110 120 PGRCHLRFDD TNPVKEEQEY IDSIQADVHW LGFDWGKNLF FASDYFDRLY EWAETLIRDG 130 140 150 160 170 180 LAYVDDQTQE EIRTSRGTLT EPGKNSPFRD RSVDENLDLF RRMKAGEFPN GARVLRAKID 190 200 210 220 230 240 MAAGNINLRD PVLYRILHAH HPRTGNAWCI YPSYDYAHGQ SDAIEGITHS ICTLEFEDHR 250 260 270 280 290 300 PLYDWFIEKL PVPSKPHQYE FARLNLTYTL LSKRVLTQLV REGHVAGWDD PRMPTMAGMR 310 320 330 340 350 360 RRGVPPAALR EFVKRIGVAK ANSVVDVGML EFCIREELNR TAQRRMAVLK PLKVVIENYP 370 380 390 400 410 420 EGQTEELEAI NHPDDPSAGT RKITFGRELY IEQDDFMENP PKKFFRLSPG NEVRLRYAYF 430 440 450 460 470 480 VKCTGVIKNE SGEVVELRCT YDPATRGGNA PDGRKVKATM HWLSAAASKP AEIRIYNQLF 490 500 510 520 530 540 ANPSPDASNF AADLNPQSLE ILSNARVEAS VAESNSTEPM QFERQGYFVR DKDSTPGKPV 550 FSRTIGLRDT FAKEVAKG

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC50102.1.
RefSeq	NP_771477.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1052143.
GenomeReviews	Gene locus bll4837 in contig BA000040_GR.
KEGG	bja:bll4837.
NMPDR	fig|224911.1.peg.4837.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89KR6.
OMA	RMPTIAG.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL4837-MON.
BRENDA	6.1.1.18. 280.
Family and domain databases
HAMAP	MF_00126. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR004514. Gln-tRNA-synth_Ic. IPR000924. Glu/Gln-tRNA-synth_Ic. IPR020061. Glu/Gln-tRNA-synth_Ic_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ic_cat-dom. IPR020059. Glu/Gln-tRNA-synth_Ic_codon-bd. IPR020060. Glu/Gln-tRNA-synth_Ic_N. IPR020056. Rbsml_L25/Gln-tRNA_synth_b-brl. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:2.40.240.10. Rbsml_L25/Gln-tRNA_synth_b-brl. 1 hit.
PANTHER	PTHR10119:SF3. GlnS. 1 hit. PTHR10119. Glu_tRNA-synt_1c. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. PF03950. tRNA-synt_1c_C. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
TIGRFAMs	TIGR00440. glnS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYQ_BRAJA

Accession

Primary (citable) accession number: Q89KR6

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2004
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)