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Q89KR5

- SYE_BRADU

UniProt

Q89KR5 - SYE_BRADU

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei243 – 2431ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBJAP224911:GJEJ-4871-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:blr4838
OrganismiBradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)
Taxonomic identifieri224911 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBradyrhizobiaceaeBradyrhizobium
ProteomesiUP000002526: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 475475Glutamate--tRNA ligasePRO_0000119521Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi224911.blr4838.

Structurei

3D structure databases

ProteinModelPortaliQ89KR5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi240 – 2445"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
InParanoidiQ89KR5.
KOiK01885.
OrthoDBiEOG6DRPF7.
PhylomeDBiQ89KR5.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q89KR5-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTDSVVTRFA PSPTGFLHIG GARTALFNWL YAKKHGGKML LRIEDTDRER
60 70 80 90 100
STEAAIGAIL DGLKWLELGW DGDVIYQFSR AARHREVAEQ LLADGKAYRC
110 120 130 140 150
YATAEELTAM REKARAEGRT RLYDGMWRDR DPATAPSDVK PTIRLRAPQT
160 170 180 190 200
GETVIEDQVQ GRVVWQNENL DDLVLLRGDG NPTYMLAVVV DDHDMGVTHV
210 220 230 240 250
IRGDDHLINA ARQKQIHDAM GWALPSMSHI PLIHGPDGSK LSKRHGALGV
260 270 280 290 300
DAYRAMGYLP AALRNYLVRL GWSHGDQEIF STEEMIAAFD LSSVGRAAAR
310 320 330 340 350
FDFAKLENLN GHYIRHADDQ SLVKMFEDVL DHVVPSRDEL KAKLNDTTRA
360 370 380 390 400
QLLKAMPALK ERAKTLIELI DSAYFIFADR PLELDPKAQA LLTPDNRKLI
410 420 430 440 450
GQLHSALENV ETWSGATTEA ALRAFAEENS LKLGAVAQPL RAALTGRTTS
460 470
PGIFEVLDVL GRQESLGRLK DQAKD
Length:475
Mass (Da):52,789
Last modified:June 1, 2003 - v1
Checksum:i0401019B9613914D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC50103.1.
RefSeqiNP_771478.1. NC_004463.1.

Genome annotation databases

EnsemblBacteriaiBAC50103; BAC50103; BAC50103.
GeneIDi1052142.
KEGGibja:blr4838.
PATRICi21193336. VBIBraJap65052_4917.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000040 Genomic DNA. Translation: BAC50103.1 .
RefSeqi NP_771478.1. NC_004463.1.

3D structure databases

ProteinModelPortali Q89KR5.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 224911.blr4838.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC50103 ; BAC50103 ; BAC50103 .
GeneIDi 1052142.
KEGGi bja:blr4838.
PATRICi 21193336. VBIBraJap65052_4917.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252722.
InParanoidi Q89KR5.
KOi K01885.
OrthoDBi EOG6DRPF7.
PhylomeDBi Q89KR5.

Enzyme and pathway databases

BioCyci BJAP224911:GJEJ-4871-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110.

Entry informationi

Entry nameiSYE_BRADU
AccessioniPrimary (citable) accession number: Q89KR5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: June 1, 2003
Last modified: October 29, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3