Reviewed, UniProtKB/Swiss-Prot Q89KQ7 (LPXB_BRAJA)

Names and origin

Protein names

Recommended name:
    Lipid-A-disaccharide synthase
    EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	bll4847

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	392 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell By similarity.
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP MF_00392
Pathway	Glycolipid biosynthesis; lipid (IV)A biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-D-glucosamine: step 5/6. HAMAP MF_00392
Sequence similarities	Belongs to the lpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 392	392	Lipid-A-disaccharide synthase HAMAP MF_00392		PRO_0000190155

Sequences

	Sequence		Length	Mass (Da)
	Q89KQ7-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: 7956C1EBD78B9C73	FASTA	392	43,262
	10 20 30 40 50 60 MMQGRDPKRK IFLIATEESG DRLGSALMKV LRQRLGDGVQ FEGVGGRTMA REGLETLFPI 70 80 90 100 110 120 EELSIVGFAA VVQQLPKILR LIRETADAVL EAVPDALVII DSPDFTHRVA RRVRARNPAI 130 140 150 160 170 180 PIVDYVSPQL WAWRPGRART MLGYVDHVLG LLPFEPEEYR KLGGPPCSYV GHPLIEQLGS 190 200 210 220 230 240 LRPNAEEQKR RNSELPVLLV LPGSRRSEIR HHIEVFGAAL GRLQAEGRAF ELMLPTMPHL 250 260 270 280 290 300 EATVREGIAS WPVKPQIVIG EAEKRAAFRI AHAALAKSGT VTLELALSGI PMVTAYRVGA 310 320 330 340 350 360 IEAFILRRAI RVSSVILANL VIGEDVIPEF LQEDCTPEKL APALSEVLTD SDMRRRQVEA 370 380 390 FARLDTIMST GNKAPSVLAA DIVLATMRKG RR

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC50112.1.
RefSeq	NP_771487.1.
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT19. Glycosyltransferase Family 19.
Genome annotation databases
GeneID	1052130.
GenomeReviews	Gene locus bll4847 in contig BA000040_GR.
KEGG	bja:bll4847.
NMPDR	fig|224911.1.peg.4847.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89KQ7.
OMA	Q89KQ7. DFTHRVA.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL4847-MON.
BRENDA	2.4.1.182. 280.
Family and domain databases
HAMAP	MF_00392. [Tree]
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_BRAJA

Accession

Primary (citable) accession number: Q89KQ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	June 1, 2003
Last modified:	June 16, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)