Reviewed, UniProtKB/Swiss-Prot Q89KG1 (CLPP2_BRAJA)

Names and origin

Protein names

Recommended name:
    ATP-dependent Clp protease proteolytic subunit 2
    EC=3.4.21.92
Alternative name(s):
    Endopeptidase Clp 2

Gene names

Name:	clpP2
Ordered Locus Names:	bll4944

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	211 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cleaves peptides in various proteins in a process that requires ATP hydrolysis. Has a chymotrypsin-like activity. Plays a major role in the degradation of misfolded proteins By similarity.
Catalytic activity	Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs). HAMAP MF_00444
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S14 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase Protease Serine protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 211	211	ATP-dependent Clp protease proteolytic subunit 2 HAMAP MF_00444		PRO_0000179515
Sites
	Active site	106	1	By similarity
	Active site	131	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89KG1-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: DF7A75EC38AE48D6	FASTA	211	23,510
	10 20 30 40 50 60 MRDPVETYMN LVPMVVEQTN RGERAYDIFS RLLKERIIFL TGPVEDGMST LVVAQLLFLE 70 80 90 100 110 120 AENPKKEISM YINSPGGVVT SGLAIYDTMQ FIRPPVSTLC TGQAASMGSL LLAAGEKDMR 130 140 150 160 170 180 FSLPNARIMV HQPSGGFQGQ ATDIMLHAQE ILNLKKRLNE IYVKHTGQTY KTIEDALERD 190 200 210 KFLTANDAKE FGLVDRVIDK RAEEPAAAKT Q

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC50209.1.
RefSeq	NP_771584.1.
3D structure databases
HSSP	HSSP built from PDB template 1TYF based on UniProtKB P19245.
ModBase	Search...
Protein family/group databases
MEROPS	S14.001.
Genome annotation databases
GeneID	1051986.
GenomeReviews	Gene locus bll4944 in contig BA000040_GR.
KEGG	bja:bll4944.
NMPDR	fig|224911.1.peg.4944.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89KG1.
OMA	VEQTAKG.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL4944-MON.
BRENDA	3.4.21.92. 280.
Family and domain databases
HAMAP	MF_00444. [Tree]
InterPro	IPR001907. Pept_S14_ClpP. IPR018215. Pept_S14_ClpP_AS. [Graphical view]
PANTHER	PTHR10381. Pept_S14_ClpP. 1 hit.
Pfam	PF00574. CLP_protease. 1 hit. [Graphical view]
PRINTS	PR00127. CLPPROTEASEP.
TIGRFAMs	TIGR00493. clpP. 1 hit.
PROSITE	PS00382. CLP_PROTEASE_HIS. 1 hit. PS00381. CLP_PROTEASE_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CLPP2_BRAJA

Accession

Primary (citable) accession number: Q89KG1

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)