Reviewed, UniProtKB/Swiss-Prot Q89K83 (HPPA_BRAJA)

Names and origin

Protein names

Recommended name:
    Pyrophosphate-energized proton pump
    EC=3.6.1.1
Alternative name(s):
    Pyrophosphate-energized inorganic pyrophosphatase
      Short name=H(+)-PPase
    Membrane-bound proton-translocating pyrophosphatase

Gene names

Name:	hppA
Ordered Locus Names:	bll5026

Organism

Bradyrhizobium japonicum [Complete proteome] [HAMAP]

Taxonomic identifier

375 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Bradyrhizobiaceae › Bradyrhizobium

Protein attributes

Sequence length	706 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Generates a proton motive force; it probably catalyzes a fully reversible reaction, thus being able to synthesize pyrophosphate when the proton motive force is sufficient By similarity.
Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_01130
Cofactor	Magnesium By similarity.
Subunit structure	Homodimer Potential.
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Sequence similarities	Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. Type 2 subfamily. [View classification]

Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Transport
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Ligand	Magnesium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proton transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular function	hydrogen-translocating pyrophosphatase activity Inferred from electronic annotation. Source: InterPro inorganic diphosphatase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 706	706	Pyrophosphate-energized proton pump HAMAP MF_01130		PRO_0000217010
Regions
	Transmembrane	4 – 26	23	Potential
	Transmembrane	57 – 79	23	Potential
	Transmembrane	123 – 145	23	Potential
	Transmembrane	158 – 180	23	Potential
	Transmembrane	231 – 250	20	Potential
	Transmembrane	262 – 281	20	Potential
	Transmembrane	296 – 318	23	Potential
	Transmembrane	331 – 353	23	Potential
	Transmembrane	376 – 398	23	Potential
	Transmembrane	403 – 425	23	Potential
	Transmembrane	469 – 486	18	Potential
	Transmembrane	516 – 538	23	Potential
	Transmembrane	585 – 607	23	Potential
	Transmembrane	614 – 636	23	Potential
	Transmembrane	682 – 704	23	Potential
Sites
	Site	464	1	Determinant of potassium independence By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q89K83-1 [UniParc]. Last modified June 1, 2003. Version 1. Checksum: C160194BAC0237F6	FASTA	706	72,442
	10 20 30 40 50 60 MTALWLIVLC GVLSVVYAIW ATSSVLSADA GSPRMQEIAA AVREGAQAYL RRQYTTIGIV 70 80 90 100 110 120 GIVIFVLLVY FLGFYVAIGF AIGAILSGAA GFIGMNVSVR ANVRTAQAAT TSLAGGLELA 130 140 150 160 170 180 FKAGAITGML VAGLALLGVT LYFGFLVYSL KLAPDSRVVV DAMVALGFGA SLISIFARLG 190 200 210 220 230 240 GGIFTKGADV GGDLVGKVEA GIPEDDPRNP ATIADNVGDN VGDCAGMAAD LFETYAVTAV 250 260 270 280 290 300 ATMVLAAIFF AKTPILMSMM TLPLAIGGIC IITSIIGTFF VKLGPSQSIM GALYKGLIAT 310 320 330 340 350 360 GVLSLIGIAV VIYTLIGFGK LDGVDYTGMS LFECGVVGLI VTALIIWITE YYTGTDYRPV 370 380 390 400 410 420 KSIAAASVTG HGTNVIQGLA ISMEATALPA IVIIAGILVT YSLAGLFGIA IATATMLALA 430 440 450 460 470 480 GMIVALDAFG PVTDNAGGIA EMAGLPKEVR KSTDALDAVG NTTKAVTKGY AIGSAGLGAL 490 500 510 520 530 540 VLFAAYNQDL KFFVADSAHH TYFAGVNPDF SLNNPYVVVG LLFGGLLPYL FGAMGMTAVG 550 560 570 580 590 600 RAASAIVEEV RRQFREKPGI MQGTDKPDYG KAVDLLTKAA IKEMIIPSLL PVLSPIVVYF 610 620 630 640 650 660 LIYAIAGGGA TGKSAAFSAV GAMLLGVIVT GLFVAISMTS GGGAWDNAKK YIEDGHYGGK 670 680 690 700 GSDAHKSAVT GDTVGDPYKD TAGPAVNPMI KITNIVALLL LAILAH

References

[1]

"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110." Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S. DNA Res. 9:189-197(2002) [PubMed: 12597275] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USDA 110.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000040 Genomic DNA. Translation: BAC50291.1.
RefSeq	NP_771666.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1051863.
GenomeReviews	Gene locus bll5026 in contig BA000040_GR.
KEGG	bja:bll5026.
NMPDR	fig|224911.1.peg.5026.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q89K83.
OMA	PYLFGGI.
Enzyme and pathway databases
BioCyc	BJAP224911:BLL5026-MON.
BRENDA	3.6.1.1. 280.
Family and domain databases
HAMAP	MF_01130. [Tree]
InterPro	IPR004131. H_PPase. [Graphical view]
Pfam	PF03030. H_PPase. 1 hit. [Graphical view]
PIRSF	PIRSF001265. H+-PPase. 1 hit.
TIGRFAMs	TIGR01104. V_PPase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HPPA_BRAJA

Accession

Primary (citable) accession number: Q89K83

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2003
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)